Abstract:
:We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin alpha, somatostatin, immunoglobulin kappa chain, alpha crystallin, albumin and interferon-beta promoters in the fission yeast Schizosaccharomyces pombe. Among these, the human cytomegalovirus, human chorionic gonadotropin alpha, and somatostatin promoters were found to be very active, approximately 11-, 9-, and 0.9-fold as active as the SV40 early promoter, respectively. The remainder of the promoters studied were weak, having only 10-20% of the SV40 promoter activity. Primer extension analysis showed that the strong promoters initiated transcription in S. pombe at the same sites as in mammalian cells, indicating the high similarity between both transcriptional systems.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Toyama R,Okayama Hdoi
10.1016/0014-5793(90)81012-dsubject
Has Abstractpub_date
1990-07-30 00:00:00pages
217-21issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(90)81012-Djournal_volume
268pub_type
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