Tyrosine phosphorylation and translocation of LAT in platelets.

abstract：:In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60-100 microM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3 degrees C) for at least 10 weeks after isolation (mildly oxidised or minimally-modifi...

journal_title：FEBS letters

authors： Stait SE,Leake DS

更新日期：1994-03-21 00:00:00

abstract：:Selenium-dependent glutathione peroxidase activity is documented for the first time in insects. Reduction in glutathione peroxidase activity in the cytosol of adult house flies by lowering selenium in the diet results in significant increases in peroxidative injury. Catalase activity, while higher in low-selenium flie...

journal_title：FEBS letters

authors： Simmons TW,Jamall IS,Lockshin RA

更新日期：1987-06-29 00:00:00

abstract：:Basic fibroblast growth factor (FGF-2) is an important modulator of cell growth and differentiation under both physiological and pathological conditions. Until recently, most investigations into the FGF-2 signalling pathway were concerned with its interaction with specific membrane receptors. Nevertheless, while a 18 ...

journal_title：FEBS letters

authors： Delrieu I

更新日期：2000-02-18 00:00:00

abstract：:The cloning and analysis of a cDNA clone encoding the soybean metalloproteinase obtained by polymerase chain reaction (PCR) and the rapid amplification of cDNA ends (RACE) reaction are described. The cDNA was constructed from poly(A)+ RNA isolated from 15-17 day old leaves. The deduced amino acid sequence of the cDNA ...

journal_title：FEBS letters

authors： Pak JH,Liu CY,Huangpu J,Graham JS

更新日期：1997-03-10 00:00:00

abstract：:Methicillin-resistant clinical isolates of Staphylococcus aureus are intrinsically resistant to beta-lactam antibiotics in that the resistance mechanism is unrelated to the possession of beta-lactamases. We have demonstrated that a new, high-molecular-mass penicillin-binding protein (PBP) is present in these strains w...

journal_title：FEBS letters

authors： Reynolds PE,Brown DF

更新日期：1985-11-11 00:00:00

abstract：:Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin...

journal_title：FEBS letters

authors： Kumari S,Dash D

更新日期：2011-07-21 00:00:00

abstract：:We have identified a cDNA encoding a 212 amino acid protein (Nm23-H5) with 27-31% identity to the human members of the nm23/nucleoside diphosphate (NDP) kinase gene family. The nm23-H5 gene is located on chromosome 5q23-31 and is transcribed as one main transcript of 1.1 kb which is highly and specifically expressed i...

journal_title：FEBS letters

authors： Munier A,Feral C,Milon L,Pinon VP,Gyapay G,Capeau J,Guellaen G,Lacombe ML

更新日期：1998-09-04 00:00:00

abstract：:A protein factor which inhibited adenylate cyclase was purified to apparent homogeneity from rat brain and identified as the beta gamma-subunits of the GTP-binding regulatory proteins of adenylate cyclase. (i) The beta gamma-subunits (protein factor) inhibited the partially purified catalytic unit of adenylate cyclase...

journal_title：FEBS letters

authors： Enomoto K,Asakawa T

更新日期：1986-06-23 00:00:00

abstract：:Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

journal_title：FEBS letters

authors： Kolchanov NA,Solovyov VV,Rogozin IB

更新日期：1987-04-06 00:00:00

abstract：:Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1989-06-05 00:00:00

abstract：:Hypermethylated in cancer, a new candidate tumor suppressor gene located in 17p13.3, encodes a protein with five Krüppel-like C2H2 zinc finger motifs and a N-terminal protein/protein interaction domain called broad complex, tramtrack and bric à brac/poxviruses and zinc finger domain. Hypermethylated in cancer appears ...

journal_title：FEBS letters

authors： Guerardel C,Deltour S,Leprince D

更新日期：1999-05-28 00:00:00

abstract：:Interfering RNA was used to suppress the expression of the genes At1g06680 and At2g30790 in Arabidopsis thaliana, which encode the PsbP-1 and PsbP-2 proteins, respectively, of Photosystem II. A phenotypic series of transgenic plants was recovered that expressed intermediate and low amounts of PsbP. Earlier we had docu...

journal_title：FEBS letters

authors： Yi X,Hargett SR,Frankel LK,Bricker TM

更新日期：2009-06-18 00:00:00

abstract：:Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...

journal_title：FEBS letters

authors： Schork SM,Bee G,Thumm M,Wolf DH

更新日期：1994-08-01 00:00:00

abstract：:The heterogeneity of computationally predicted reaction fluxes in metabolic networks within a single flux state can be exploited to detect their significant flux backbone. Here, we disclose the backbone of Escherichia coli, and compare it with the backbones of other bacteria. We find that, in general, the core of the ...

journal_title：FEBS letters

authors： Güell O,Sagués F,Serrano MÁ

更新日期：2017-05-01 00:00:00

abstract：:Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...

journal_title：FEBS letters

authors： McGregor GP,Hartel R,Fehmann HC,Lankat-Buttgereit B,Göke B,Göke R,Voigt K

更新日期：1992-11-09 00:00:00

abstract：:1H NMR spectra of skinned rabbit muscle fibers show a group of relatively sharp resonance lines which presumably originate from highly mobile protein domains. Comparison with the spectrum of myosin subfragment 1 suggests that these signals may come at least partly from mobile regions of the myosin head. NMR could poss...

journal_title：FEBS letters

authors： Kalbitzer HR,Schrumpf M,Wray J

更新日期：1992-02-24 00:00:00

abstract：:We have studied the incorporation of [(14)C]serine and of [(3)H]sphingosine into sphingomyelin in the presence or absence of brefeldin A (BFA) in three different cell types. Administration of BFA (1 microgram/ml) to fibroblasts for 24 h increased the incorporation of label into sphingomyelin 1.5-3 fold compared with u...

journal_title：FEBS letters

authors： Sadeghlar F,Sandhoff K,van Echten-Deckert G

更新日期：2000-07-28 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:Escherichia coli heat-shock proteins GroEL and GroES stimulate (in an ATP-dependent manner) the folding of various proteins. In this study scanning microcalorimetry was applied to investigate GroEL thermostability in the presence of its ligands. Mg2+ and K+ ions stabilize while ADP destabilizes the GroEL molecule agai...

journal_title：FEBS letters

authors： Surin AK,Kotova NV,Kashparov IA,Marchenkov VV,Marchenkova SYu,Semisotnov GV

更新日期：1997-04-01 00:00:00

abstract：:Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved meth...

journal_title：FEBS letters

authors： Loir M,Caraty A,Lanneau M,Menezo Y,Muh JP,Sautiere P

更新日期：1984-04-24 00:00:00

abstract：:A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...

journal_title：FEBS letters

authors： Murakami R,Ifuku K,Takabayashi A,Shikanai T,Endo T,Sato F

更新日期：2002-07-17 00:00:00

abstract：:Localisation of mRNA encoding the four AMPA glutamate receptor subunits in the rat adrenal gland was investigated using in situ hybridisation. GluR1 is found in the zona glomerulosa of the cortex, GluR3 in the remaining parts of the cortex, GluR2 in adrenal medullary cells and GluR4 at a very low level in the zona glo...

journal_title：FEBS letters

authors： Kristensen P

更新日期：1993-10-11 00:00:00

abstract：:A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...

journal_title：FEBS letters

authors： Mizuno K,Noda K,Ueda Y,Hanaki H,Saido TC,Ikuta T,Kuroki T,Tamaoki T,Hirai S,Osada S

更新日期：1995-02-13 00:00:00

abstract：:Purified plasma membrane vesicles isolated from multidrug-resistant human KB-V1 cells accumulate [3H]vinblastine in an energy-dependent manner. The accumulation of [3H]vinblastine in the presence of ATP is a saturable process. ATP can be replaced by other purine nucleotide triphosphates, of which GTP is the most effic...

journal_title：FEBS letters

authors： Lelong IH,Padmanabhan R,Lovelace E,Pastan I,Gottesman MM

更新日期：1992-06-15 00:00:00

abstract：:Different hydrophobic glycoproteins are associated to native biliary vesicles, which are the major carrier of biliary cholesterol. Some of these proteins promote cholesterol crystallization, a key step in cholesterol gallstone formation. This study was specifically conducted to identify the 130 kDa biliary vesicle-ass...

journal_title：FEBS letters

authors： Núñez L,Amigo L,Rigotti A,Puglielli L,Mingrone G,Greco AV,Nervi F

更新日期：1993-08-23 00:00:00

abstract：:Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...

journal_title：FEBS letters

authors： Nikolaev LG,Glotov BO,Dashkevich VK,Barbashov SF,Severin ES

更新日期：1983-10-31 00:00:00

abstract：:The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoin...

journal_title：FEBS letters

authors： Uebele VN,Yeola SW,Snyders DJ,Tamkun MM

更新日期：1994-02-28 00:00:00

abstract：:Stimulation of monocytes/macrophages with lipopolysaccharide (LPS) results in activation of nuclear factor-kappaB (NF-kappaB), which plays crucial roles in regulating expression of many genes involved in the subsequent inflammatory responses. Here, we investigated roles of transforming growth factor-beta activated kin...

journal_title：FEBS letters

authors： Irie T,Muta T,Takeshige K

更新日期：2000-02-11 00:00:00

abstract：:α-Synuclein (αSyn) is a key player in the pathogenesis of Parkinson's disease and other synucleinopathies. Here, we report the existence of a novel soluble α-helical conformer of αSyn, obtained through transient interaction with lipid interfaces, and propose dynamic oligomerization as the mechanism underlying its stab...

journal_title：FEBS letters

authors： Rovere M,Sanderson JB,Fonseca-Ornelas L,Patel DS,Bartels T

更新日期：2018-05-01 00:00:00

abstract：:The 3D structure of two unlabeled FK506 analogs, (R)- and (S)-[18-OH]ascomycin, when bound to [U-13C,15N]FKBP were determined by isotope-filtered 2D NMR experiments. The structures for the R and S isomers that bind tightly to FKBP but lack immunosuppressive activity are compared to each other and to the conformation o...

journal_title：FEBS letters

authors： Petros AM,Kawai M,Luly JR,Fesik SW

更新日期：1992-08-24 00:00:00