Demonstration and partial purification of lactoperoxidase from human colostrum.

abstract：:LH-stimulated adenylate cyclase activity in membrane preparations of bovine luteal cells could be enhanced by treating the cells with either phospholipase D or its hydrolysis product, phosphatidic acid. Similar augmentary effects were also produced following treatment of the cells with EGF. Moreover, EGF could stimula...

journal_title：FEBS letters

authors： Budnik LT,Mukhopadhyay AK

更新日期：1993-07-12 00:00:00

abstract：:The lipid A structure of the Gram-negative bacterium Helicobacter mustelae, a ferret gastric pathogen responsible for the onset of gastric diseases in its host, was investigated. Two variant lipid A structures were found in the same strain. One structure contained a bisphosphorylated beta-(1-->6)-linked D-glucosamine ...

journal_title：FEBS letters

authors： Thérisod H,Monteiro MA,Perry MB,Caroff M

更新日期：2001-06-15 00:00:00

abstract：:Sequence comparisons among methionyl-tRNA synthetases from different organisms reveal only one block of homology beyond the last beta strand of the mononucleotide fold. We have introduced a series of semi-conservative amino acid replacements in the conserved motif of yeast methionyl-tRNA synthetase. The results indica...

journal_title：FEBS letters

authors： Despons L,Walter P,Senger B,Ebel JP,Fasiolo F

更新日期：1991-09-09 00:00:00

abstract：:Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

journal_title：FEBS letters

authors： Swain J,Gutteridge JM

更新日期：1995-07-24 00:00:00

abstract：:The alphavirus-like mRNA capping enzyme of Bamboo mosaic virus (BaMV) exhibits an AdoMet-dependent guanylyltransferase activity by which the methyl group of AdoMet is transferred to GTP, leading to the formation of m(7)GTP, and the m(7)GMP moiety is next transferred to the 5' end of ppRNA via a covalent enzyme-m(7)GMP...

journal_title：FEBS letters

authors： Lin HY,Yu CY,Hsu YH,Meng M

更新日期：2012-07-30 00:00:00

abstract：:Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...

journal_title：FEBS letters

authors： Binda C,Hubálek F,Li M,Edmondson DE,Mattevi A

更新日期：2004-04-30 00:00:00

abstract：:Lectin-like oxidized low-density lipoprotein receptor (LOX-1) is a scavenger receptor that binds oxidized low-density lipoprotein (OxLDL) and has a role in atherosclerosis development. The N-terminus intracellular region (cytoplasmic domain) of LOX-1 mediates receptor internalization and trafficking, potentially throu...

journal_title：FEBS letters

authors： Bakthavatsalam D,Soung RH,Tweardy DJ,Chiu W,Dixon RA,Woodside DG

更新日期：2014-06-13 00:00:00

abstract：:Biological systems, from macromolecules to whole organisms, are robust if they continue to function, survive, or reproduce when faced with mutations, environmental change, and internal noise. I focus here on biological systems that are robust to mutations and ask whether such systems are more or less evolvable, in the...

journal_title：FEBS letters

authors： Wagner A

更新日期：2005-03-21 00:00:00

abstract：:The protein translocation apparatus in Escherichia coli has been studied both genetically and biochemically. In vitro protein translocation systems involving everted membrane vesicles or reconstituted proteoliposomes have significantly contributed to biochemical clarification of the structure, mechanism and energetics...

journal_title：FEBS letters

authors： Tokuda H

更新日期：1994-06-06 00:00:00

abstract：:It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...

journal_title：FEBS letters

authors： Kandror KV,Kapkov DV,Turapov OA,Stepanov AS

更新日期：1991-06-03 00:00:00

abstract：:The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...

journal_title：FEBS letters

authors： Vengerov YY,Semenov TE,Streltsov SA,Makarov VL,Khorlin AA,Gursky GV

更新日期：1985-01-21 00:00:00

abstract：:32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphati...

journal_title：FEBS letters

authors： Mauco G,Chap H,Douste-Blazy L

更新日期：1983-03-21 00:00:00

abstract：:TGF-β family signaling through Smads is conceptually a simple and linear signaling pathway, driven by sequential phosphorylation, with type II receptors activating type I receptors, which in turn activate R-Smads. Nevertheless, TGF-β family proteins induce highly complex programs of gene expression responses that are ...

journal_title：FEBS letters

authors： Xu P,Liu J,Derynck R

更新日期：2012-07-04 00:00:00

abstract：:Effects of sulfatide and its precursor galactosylceramide (gal-cer) on the kinetics of production of cytokines were studied. In human mononuclear leucocytes, gal-cer but not sulfatide induced significantly increased amounts of interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF) mRNA. In phytohemagglutinin-sti...

journal_title：FEBS letters

authors： Bovin LF,Fredman P,Månsson JE,Buschard K,Bendtzen K

更新日期：1999-07-23 00:00:00

abstract：:Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels incre...

journal_title：FEBS letters

authors： Schmidt O,Weyer Y,Fink MJ,Müller M,Weys S,Bindreither M,Teis D

更新日期：2017-09-01 00:00:00

abstract：:The PDE4 catalytic machinery comprises, in part, two divalent cations in a binuclear motif. Here we report that PDE4A4 expressed in Sf9 cells exhibits a biphasic Mg(2+) dose-response (EC(50) of 0.15 and >10 mM) in catalyzing cAMP hydrolysis. In vitro phosphorylation of PDE4A4 by the PKA-catalytic subunit increases the...

journal_title：FEBS letters

authors： Laliberté F,Liu S,Gorseth E,Bobechko B,Bartlett A,Lario P,Gresser MJ,Huang Z

更新日期：2002-02-13 00:00:00

abstract：:PKD-mediated phosphorylation of class IIa HDACs frees the MEF2 transcription factor to activate genes that govern muscle differentiation and growth. Studies of the regulation and function of this signaling axis have involved MC1568 and Gö-6976, which are small molecule inhibitors of class IIa HDAC and PKD catalytic ac...

journal_title：FEBS letters

authors： Lemon DD,Harrison BC,Horn TR,Stratton MS,Ferguson BS,Wempe MF,McKinsey TA

更新日期：2015-04-28 00:00:00

abstract：:In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...

journal_title：FEBS letters

authors： Mruthyunjaya S,Rumma M,Ravibhushan G,Anjali S,Padma S

更新日期：2011-06-23 00:00:00

abstract：:The phosphatidylinositol 4,5-bisphosphate (PtdIns-P2)-regulated actin-binding protein gelsolin and most phosphoinositide-specific phospholipases C (PLCs) comprise a basic amino acid motif ((K/R)xxxKxK(K/R); x denotes any amino acid) which was previously suggested to represent a PtdInsP2-binding site commonly present i...

journal_title：FEBS letters

authors： Simões AP,Camps M,Schnabel P,Gierschik P

更新日期：1995-05-29 00:00:00

abstract：:The assembly of the Sm-class of uridine-rich small nuclear ribonucleoproteins (U snRNPs), albeit spontaneous in vitro, has recently been shown to be dependent on the aid of a large number of assisting factors in vivo. These factors are organized in two interacting units termed survival motor neuron (SMN)- and protein ...

journal_title：FEBS letters

authors： Neuenkirchen N,Chari A,Fischer U

更新日期：2008-06-18 00:00:00

abstract：:Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of p...

journal_title：FEBS letters

authors： McCarthy L,Bentley-DeSousa A,Denoncourt A,Tseng YC,Gabriel M,Downey M

更新日期：2020-01-01 00:00:00

abstract：:A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...

journal_title：FEBS letters

authors： Heinemann SH,Rettig J,Wunder F,Pongs O

更新日期：1995-12-27 00:00:00

abstract：:Post-absorptive gluconeogenesis from lactate measured in vivo increases 3-fold in 24-month-old rats compared to 3-month-old animals. Fractional lactate turnover rates showed no significant differences between the two groups of animals. Lower plasma glucose concentrations and insulin-glucagon ratios may explain the inc...

journal_title：FEBS letters

authors： Satrústegui J,Cuezva JM,Machado A

更新日期：1986-03-03 00:00:00

abstract：:Structural and biochemical studies have begun to elucidate the pathway of RNA silencing that leads to the formation of the RISC complex. The outstanding feature of this pathway is the precise recognition and processing of double-stranded RNA. We present a review of recent structures that illustrate the molecular mecha...

journal_title：FEBS letters

authors： Collins RE,Cheng X

更新日期：2005-10-31 00:00:00

abstract：:Tau proteins aggregate into different neuronal inclusions in several neurodegenerative disorders. In Alzheimer's disease (AD), hyperphosphorylated Tau from paired helical filaments (PHF) of neurofibrillary tangles, named PHF-Tau, have an electrophoretic profile with four main bands (Tau 55, 64, 69, 74 kDa). In Pick's ...

journal_title：FEBS letters

authors： Sergeant N,David JP,Lefranc D,Vermersch P,Wattez A,Delacourte A

更新日期：1997-08-04 00:00:00

abstract：:A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a startin...

journal_title：FEBS letters

authors： Osborne S,Cecconato E,Griva S,Garetto F,Calogero R,Rosa C,Bonelli F

更新日期：1993-06-21 00:00:00

abstract：:Botulinum neurotoxins (BoNTs), the causative agent of human botulism, are the most potent naturally occurring toxins known. BoNT/A1, the most studied BoNT, is also used as an important biopharmaceutical. In this study, the biological activity of BoNT/A1 is compared to that of BoNT/A2 using neuronal cell models. The da...

journal_title：FEBS letters

authors： Pier CL,Chen C,Tepp WH,Lin G,Janda KD,Barbieri JT,Pellett S,Johnson EA

更新日期：2011-01-03 00:00:00

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...

journal_title：FEBS letters

authors： Comis A,Easterbrook-Smith SB

更新日期：1986-03-03 00:00:00

abstract：:The up-regulation of HER2/neu is associated with human malignancies and is a useful target for developing anticancer drugs. Overexpression of human manganese superoxide dismutase (MnSOD) has been demonstrated to effectively suppress various carcinoma cells, including breast carcinomas, in vitro and in vivo. This study...

journal_title：FEBS letters

authors： Chuang TC,Liu JY,Lin CT,Tang YT,Yeh MH,Chang SC,Li JW,Kao MC

更新日期：2007-09-18 00:00:00