Dynamic structure of pharaonis phoborhodopsin (sensory rhodopsin II) and complex with a cognate truncated transducer as revealed by site-directed 13C solid-state NMR.

abstract：:The transport of proteins from the cytoplasm into the nucleus is a multistep process. The nuclear localization sequence (NLS) of a transport substrate associates with the heterodimeric NLS-receptor which binds to a subset of proteins of the nuclear pore complex (NPC). Translocation through the NPC is energy-dependent ...

journal_title：FEBS letters

authors： Schlenstedt G

更新日期：1996-06-24 00:00:00

abstract：:The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. I...

journal_title：FEBS letters

authors： Ling KK,Siow NL,Choi RC,Tsim KW

更新日期：2005-04-25 00:00:00

abstract：:We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery...

journal_title：FEBS letters

authors： Hasan MR,Jin M,Matsushima K,Miyamoto S,Kotani S,Nakagawa H

更新日期：2006-06-12 00:00:00

abstract：:T-kininogen is known to be an acute-phase reactant as well as a kininogen in rat plasma. Three kinds of cytokines, interleukin-1, tumor necrosis factor and interferon, were assayed for their abilities to stimulate hepatic production of T-kininogen. Of these cytokines, interferon was able to stimulate hepatic productio...

journal_title：FEBS letters

authors： Itoh N,Yayama K,Okamoto H

更新日期：1988-03-14 00:00:00

abstract：:The α-subunit of the cardiac voltage-gated sodium channel (NaV1.5) plays a central role in cardiomyocyte excitability. We have recently reported that NaV1.5 is post-translationally modified by arginine methylation. Here, we aimed to identify the enzymes that methylate NaV1.5, and to describe the role of arginine methy...

journal_title：FEBS letters

authors： Beltran-Alvarez P,Espejo A,Schmauder R,Beltran C,Mrowka R,Linke T,Batlle M,Pérez-Villa F,Pérez GJ,Scornik FS,Benndorf K,Pagans S,Zimmer T,Brugada R

更新日期：2013-10-01 00:00:00

abstract：:Hepatocyte tight junctional permeability has been shown to be regulated by hormones that exert their effects via phospholipase C activation. However, the precise transduction pathway involved in this effect is not known. The present study has employed the selective inhibitor of microsomal Ca2+ sequestration, 2,5-di(te...

journal_title：FEBS letters

authors： Llopis J,Kass GE,Duddy SK,Farell GC,Gahm A,Orrenius S

更新日期：1991-03-11 00:00:00

abstract：:The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

journal_title：FEBS letters

authors： Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

更新日期：1999-07-02 00:00:00

abstract：:Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...

journal_title：FEBS letters

authors： Dehio M,Gómez-Duarte OG,Dehio C,Meyer TF

更新日期：1998-03-06 00:00:00

abstract：:13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...

journal_title：FEBS letters

authors： Legrand N,Bondon A,Simonneaux G,Jung C,Gill E

更新日期：1995-05-08 00:00:00

abstract：:Skeletal muscle plays key roles in metabolic homeostasis. Loss of muscle mass, called muscle atrophy exacerbates disease-associated metabolic perturbations. In this study, we characterized the molecular functions and mechanisms underlying regulation of skeletal muscle atrophy induced by denervation. Denervation signif...

journal_title：FEBS letters

authors： Kang J,Jeong MG,Oh S,Jang EJ,Kim HK,Hwang ES

更新日期：2014-01-03 00:00:00

abstract：:As a result of Myc-dependent transcription of the LDH-A gene, Myc-transformed cells (Rat1-Myc) exhibit increased lactate production rates (LPR) even under aerobic conditions (the Warburg effect). Recently, the increased susceptibility to stress-induced apoptosis associated with Myc transfection has been linked to the ...

journal_title：FEBS letters

authors： Papas KK,Sun L,Roos ES,Gounarides JS,Shapiro M,Nalin CM

更新日期：1999-03-12 00:00:00

abstract：:Anaerobic biosynthesis of carotenoids in the purple facultative photosynthetic bacterium Rhodobacter sphaeroides was studied using mass spectrometry. We have demonstrated that (18)O from H2(18)O was incorporated into the 1-methoxy group of spheroidene and spheroidenone, the two major carotenoids produced by this bacte...

journal_title：FEBS letters

authors： Yeliseev AA,Kaplan S

更新日期：1997-02-10 00:00:00

abstract：:Abnormal retention of Delta F508 CFTR (cystic fibrosis transmembrane conductance regulator) in the endoplasmic reticulum is a major cause of cystic fibrosis (CF). We show that calnexin Delta 185-520 but not calnexin can partially reverse the mislocalization of Delta F508 CFTR. This 256-amino acid protein has neither t...

journal_title：FEBS letters

authors： Okiyoneda T,Wada I,Jono H,Shuto T,Yoshitake K,Nakano N,Nagayama S,Harada K,Isohama Y,Miyata T,Kai H

更新日期：2002-08-28 00:00:00

abstract：:Endothelin (ET-1) has been shown to exert vasoconstrictor activity in vivo and mobilize Ca2+ in vascular smooth muscle cells in culture. In this paper we show that the human skin fibroblast exhibits specific receptors to ET-1 and that activation of these receptors results in increased intracellular Ca2+ (Ca2+i) and ac...

journal_title：FEBS letters

authors： Gardner JP,Maher E,Aviv A

更新日期：1989-10-09 00:00:00

abstract：:We transfected the COS-7 cells with cDNAs encoding different human somatostatin receptor (hSSTR) subtypes, and found that hSSTR subtypes mediate not only the inhibition of forskolin-induced cAMP accumulation but also the stimulation of phospholipase C (PLC) and Ca2+ mobilization. Activation of PLC by 1 microM somatost...

journal_title：FEBS letters

authors： Akbar M,Okajima F,Tomura H,Majid MA,Yamada Y,Seino S,Kondo Y

更新日期：1994-07-11 00:00:00

abstract：:We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size o...

journal_title：FEBS letters

authors： Takeshima H,Nishimura S,Nishi M,Ikeda M,Sugimoto T

更新日期：1993-05-10 00:00:00

abstract：:The sequence of the 18 S rRNA of Tenebrio molitor is reported. A detailed secondary structure model for eukaryotic small subunit rRNAs is proposed. The model comprises 48 universal helices that eukaryotic and prokaryotic small subunit rRNAs have in common, plus a number of helices in areas of variable secondary struct...

journal_title：FEBS letters

authors： Hendriks L,De Baere R,Van Broeckhoven C,De Wachter R

更新日期：1988-05-09 00:00:00

abstract：:In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å r...

journal_title：FEBS letters

authors： She Z,Gao ZQ,Liu Y,Wang WJ,Liu GF,Shtykova EV,Xu JH,Dong YH

更新日期：2012-07-30 00:00:00

abstract：:Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 ...

journal_title：FEBS letters

authors： Telfer JF,Green CD

更新日期：1993-08-30 00:00:00

abstract：:The activity of S-adenosylmethionine decarboxylase (AdoMetDC) in Crithidia fasciculata was shown to be correlated to the growth of the parasite. An increase in activity was observed during exponential growth. Inhibition of protein synthesis induced an extremely rapid decay of AdoMetDC activity. The half-life of the en...

journal_title：FEBS letters

authors： Nasizadeh S,Persson L

更新日期：2003-10-09 00:00:00

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80%, and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly re...

journal_title：FEBS letters

authors： Chessell IP,Simon J,Hibell AD,Michel AD,Barnard EA,Humphrey PP

更新日期：1998-11-13 00:00:00

abstract：:It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechan...

journal_title：FEBS letters

authors： Díaz M,Achkor H,Titarenko E,Martínez MC

更新日期：2003-05-22 00:00:00

abstract：:The amyloid precursor protein (APP) is proteolytically processed by β- and γ-secretases to release amyloid-β peptide (Aβ), the main component found in senile plaques of Alzheimer's disease (AD) patient brains. Alternatively, APP can be cleaved within the Aβ sequence by α-secretase, thus precluding the generation of Aβ...

journal_title：FEBS letters

authors： León-Otegui M,Gómez-Villafuertes R,Díaz-Hernández JI,Díaz-Hernández M,Miras-Portugal MT,Gualix J

更新日期：2011-07-21 00:00:00

abstract：:The solution structure of the catalytic domain of MMP-20, a member of the matrix metalloproteinases family not yet structurally characterized, complexed with N-Isobutyl-N-(4-methoxyphenylsulfonyl)glycyl hydroxamic acid (NNGH), is here reported and compared with other MMPs-NNGH adducts. The backbone dynamic has been ch...

journal_title：FEBS letters

authors： Arendt Y,Banci L,Bertini I,Cantini F,Cozzi R,Del Conte R,Gonnelli L

更新日期：2007-10-02 00:00:00

abstract：:The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...

journal_title：FEBS letters

authors： Baykousheva SP,Luthria DL,Sprecher H

更新日期：1995-06-26 00:00:00

abstract：:Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

journal_title：FEBS letters

authors： Ferry DR,Glossmann H

更新日期：1982-11-08 00:00:00

abstract：:When MCF-7 cells were treated with 17 beta-estradiol, dexamethasone, or promegestone at 37 degrees C, the KD of receptors for their cognate ligands was found to decrease as compared to that measured at 2 degrees C. Cell incubation with hormone at 37 degrees C did not affect the Bmax of glucocorticoid and progesterone ...

journal_title：FEBS letters

authors： Rossini GP,Malaguti C

更新日期：1995-12-04 00:00:00

abstract：:We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scat...

journal_title：FEBS letters

authors： He C,Gu Q,Huang M,Xing J,Chen J

更新日期：2013-01-31 00:00:00

abstract：:It has previously been shown that the levels of poly(ADP-ribose)polymerase and polymers of ADP-ribose that co-purify with the nuclear matrix in regenerating liver fluctuate with the levels of in vivo DNA replication [(1988) FEBS Lett. 236, 362-366]. We have now electrophoretically identified lamins A and C, and poly(A...

journal_title：FEBS letters

authors： Pedraza-Reyes M,Alvarez-Gonzalez R

更新日期：1990-12-17 00:00:00