Abstract:
:We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery after photobleaching analyses revealed that the interaction of MAP4-SP with the microtubules is very rapid, with a half-time of fluorescence recovery of 7 +/- 2.36 s, compared to 19.5 +/- 3.03 s in case of MAP4-LP. The dynamic interaction of MAP4-SP with microtubules in neural cells may contribute to the dynamic behaviors of extending neurites.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hasan MR,Jin M,Matsushima K,Miyamoto S,Kotani S,Nakagawa Hdoi
10.1016/j.febslet.2006.05.028subject
Has Abstractpub_date
2006-06-12 00:00:00pages
3505-10issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00620-Xjournal_volume
580pub_type
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