Functional identification of ATP-driven Ca2+ pump in the peribacteroid membrane of broad bean root nodules.

abstract：:The efflux of [3H]purines from cultured sympathetic neurons prelabelled with [3H]adenine is accelerated 2-3-fold within hours of nerve growth factor (NGF) withdrawal and is reduced by readdition of NGF. Addition of 8-(4-chlorphenyl-thio) cAMP, which delays neurite degeneration, reduced the enhanced efflux of purines, ...

journal_title：FEBS letters

authors： Tolkovsky AM,Buckmaster EA

更新日期：1989-09-25 00:00:00

abstract：:Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...

journal_title：FEBS letters

authors： Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

更新日期：2000-04-14 00:00:00

abstract：:Muscle phosphofructokinase interacts with microtubule-associated protein-free microtubules resulting in a reduction of the overall activity of the enzyme [Lehotzky et al. (1993) J. Biol. Chem. 268, 10888-10894] and periodical cross-linking of the tubules [Lehotzky et al. (1994) Biochem. Biophys. Res. Commun. 204, 585-...

journal_title：FEBS letters

authors： Vértessy BG,Kovács J,Ovádi J

更新日期：1996-01-29 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:Endothelin (ET-1) has been shown to exert vasoconstrictor activity in vivo and mobilize Ca2+ in vascular smooth muscle cells in culture. In this paper we show that the human skin fibroblast exhibits specific receptors to ET-1 and that activation of these receptors results in increased intracellular Ca2+ (Ca2+i) and ac...

journal_title：FEBS letters

authors： Gardner JP,Maher E,Aviv A

更新日期：1989-10-09 00:00:00

abstract：:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...

journal_title：FEBS letters

authors： Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi K

更新日期：1983-07-11 00:00:00

abstract：:We show here that phosphorylation of the insulin receptor and insulin receptor substrate-1 is increased when suspended cells are replated on fibronectin. This is not due to decreased numbers of cell surface receptors, alteration of insulin binding, or stimulation of a phosphatase activity in non-adherent cells. Expres...

journal_title：FEBS letters

authors： El Annabi S,Gautier N,Baron V

更新日期：2001-11-02 00:00:00

abstract：:Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct cis-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, delet...

journal_title：FEBS letters

authors： Oe S,Yoneda Y

更新日期：2010-08-04 00:00:00

abstract：:A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potenti...

journal_title：FEBS letters

authors： Anemüller S,Schmidt CL,Schäfer G,Teixeira M

更新日期：1993-02-22 00:00:00

abstract：:The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 ...

journal_title：FEBS letters

authors： Nakano K,Arai R,Mabuchi I

更新日期：2005-09-26 00:00:00

abstract：:The oxysterol binding proteins (OSBPs) are believed to control cholesterol homeostasis but their precise mechanism of action is not well understood. The Dictyostelium osbA gene encodes a predicted OSBP, OSBPa, which lacks the PH domain that in most other OSBPs directs targetting to the Golgi. OSBPa instead localises s...

journal_title：FEBS letters

authors： Fukuzawa M,Williams JG

更新日期：2002-09-11 00:00:00

abstract：:Tyrosyl radicals can catalyze LDL oxidation. In addition to their LDL oxidizing ability, superoxide (O2.-)/nitric oxide (NO.) generate phenoxyl radicals when reacting with tyrosine. Therefore we tested if tyrosine can act as a pro-oxidant in O2.-/NO.-initiated LDL oxidation. When LDL was exposed to O2.-/NO., tyrosine ...

journal_title：FEBS letters

authors： Kapiotis S,Hermann M,Held I,Mühl A,Gmeiner B

更新日期：1997-06-09 00:00:00

abstract：:Lipoic acid is a coenzyme essential to the activity of enzymes such as pyruvate dehydrogenase, which play important roles in central metabolism. However, neither the enzymes responsible for biosynthesis nor the biosynthetic event of lipoic acid has been reported in mammalian cells. In this study, a mouse mLIP1 cDNA fo...

journal_title：FEBS letters

authors： Morikawa T,Yasuno R,Wada H

更新日期：2001-06-01 00:00:00

abstract：:Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two-hybrid assay and co-immunoprecip...

journal_title：FEBS letters

authors： Peng X,Zhang Y,Zhang H,Graner S,Williams JF,Levitt ML,Lokshin A

更新日期：1999-03-05 00:00:00

abstract：:We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in ...

journal_title：FEBS letters

authors： Miura D,Kida Y,Nojima H

更新日期：2007-05-29 00:00:00

abstract：:Impaired functioning of the proteasome pathway is one of the molecular mechanism underlying neurodegenerative changes in Alzheimer's disease. In this study, we report that dysfunction of the proteasome pathway in astroglial cells leads to decreased survival and dysregulation of chemokines by differential regulation of...

journal_title：FEBS letters

authors： Choi K,Lee J,Choi C

更新日期：2007-10-02 00:00:00

abstract：:Acylaminoacyl peptidase is a member of the prolyl oligopeptidase family. Amino acid sequence alignment suggests that the stabilization of the tetrahedral intermediate should be mediated by His507 rather than by a tyrosine residue found in the other family members of this serine peptidase group. The pH dependence of k(...

journal_title：FEBS letters

authors： Kiss AL,Szeltner Z,Fülöp V,Polgár L

更新日期：2004-07-30 00:00:00

abstract：:It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...

journal_title：FEBS letters

authors： Kandror KV,Kapkov DV,Turapov OA,Stepanov AS

更新日期：1991-06-03 00:00:00

abstract：:The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary...

journal_title：FEBS letters

authors： Cahour A,Debeire P,Hartmann L,Montreuil J,van Halbeek H,Vliegenthart JF

更新日期：1984-05-21 00:00:00

abstract：:The membrane-membrane fusion during fertilization of oocyte by spermatozoa is believed to be mainly mediated by so called "fusion proteins". In the present study we have tried to demonstrate that beside the proteins, lipid components of membrane may play an important role in fusion of oocyte with spermatozoa. Conventi...

journal_title：FEBS letters

authors： Atif SM,Hasan I,Ahmad N,Khan U,Owais M

更新日期：2006-04-17 00:00:00

abstract：:Beta-thymosins sequester G-actin and preserve a pool of monomers of actin which constitute an important prerequisite for cellular function of the microfilament system. To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constan...

journal_title：FEBS letters

authors： Huff T,Cappelletti G,Hannappel E

更新日期：1998-04-03 00:00:00

abstract：:The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was no...

journal_title：FEBS letters

authors： Katerelos NA,Taylor MA,Scott M,Goodenough PW,Pickersgill RW

更新日期：1996-08-19 00:00:00

abstract：:The degradation of type IX collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (MMPs) purified from the culture medium of rheumatoid synovial cells. Neither MMP-1 (collagenase) nor MMP-2 (so-called 'gelatinase') could digest type IX collagen, but M...

journal_title：FEBS letters

authors： Okada Y,Konomi H,Yada T,Kimata K,Nagase H

更新日期：1989-02-27 00:00:00

abstract：:Part of the cytoplasmic domain of a human desmoglein, Dsg1, a cadherin-like protein found in desmosomes of epithelial cells, has been visualised by electron microscopy. The cloned fragment contains five repeats of a 29 +/- 4 residue sequence unique to desmogleins, followed by a glycine-rich region. In rotary shadowed ...

journal_title：FEBS letters

authors： Rutman AJ,Buxton RS,Burdett ID

更新日期：1994-10-17 00:00:00

abstract：:The thermal blockage of QA-. oxidation was analysed in PS II membrane fragments by monitoring flash-induced changes of the relative fluorescence quantum yield as a function of temperature. The results obtained reveal: (a) in dark-adapted samples the fraction of QA-. that is not reoxidised within a time domain of 10 s ...

journal_title：FEBS letters

authors： Reifarth F,Renger G

更新日期：1998-05-29 00:00:00

abstract：:C1qTNF-related proteins (CTRPs) are involved in diverse processes including metabolism, inflammation host defense, apoptosis, cell differentiation, autoimmunity, hibernation, and organogenesis. However, the physiological role of CTRP6 remains poorly understood. Here we demonstrate that the globular domain of CTRP6 med...

journal_title：FEBS letters

authors： Lee W,Kim MJ,Park EJ,Choi YJ,Park SY

更新日期：2010-03-05 00:00:00

abstract：:Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...

journal_title：FEBS letters

authors： Selvamurugan N,Shimizu E,Lee M,Liu T,Li H,Partridge NC

更新日期：2009-04-02 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) mRNA expression was analysed in rabbit vascular smooth muscle cells following exposure to hypoxia and platelet-derived growth factor-BB (PDGF-BB). Hypoxia potently upregulated VEGF mRNA steady-state levels in a time- and concentration-dependent manner reaching a maximum level ...

journal_title：FEBS letters

authors： Stavri GT,Hong Y,Zachary IC,Breier G,Baskerville PA,Ylä-Herttuala S,Risau W,Martin JF,Erusalimsky JD

更新日期：1995-01-30 00:00:00

abstract：:50 S ribosomal subunit derivatives without the 5 S RNA-protein complex obtained either by splitting with EDTA or by reconstitution from the 23 S RNA and proteins have been studied by electron microscopy. Removal of the 5 S RNA-protein complex is shown to affect neither the overall morphology of the larger ribosomal su...

journal_title：FEBS letters

authors： Selivanova OM,Gongadze GM,Gudkov AT,Vasiliev VD

更新日期：1986-03-03 00:00:00

abstract：:Flavonoid 3'-hydroxylase (F3'H) and flavonoid 3',5'-hydroxylase (F3'5'H) are cytochrome P450 enzymes and determine the B-ring hydroxylation pattern of flavonoids by introducing hydroxyl groups at the 3'- or the 3'- and 5'-position, respectively. Sequence identity between F3'H and F3'5'H is generally low since their di...

journal_title：FEBS letters

authors： Seitz C,Ameres S,Forkmann G

更新日期：2007-07-24 00:00:00