Abstract:
:The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary structure of the major compounds was elucidated on the basis of carbohydrate analysis, methylation analysis, including mass-spectrometry, and 500 MHz 1H-NMR spectroscopy. For both patients, this appeared to be a monosialyl monofucosyl biantennary structure; the compounds differed by the presence of an intersecting N-acetylglucosamine residue.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cahour A,Debeire P,Hartmann L,Montreuil J,van Halbeek H,Vliegenthart JFdoi
10.1016/0014-5793(84)81341-1subject
Has Abstractpub_date
1984-05-21 00:00:00pages
343-9issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(84)81341-1journal_volume
170pub_type
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