Abstract:
:A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1-21) and another with more hydrophilicity than hET(1-21). These data indicate that ET is a circulating vasoconstrictor hormone in man.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ando K,Hirata Y,Shichiri M,Emori T,Marumo Fdoi
10.1016/0014-5793(89)80213-3subject
Has Abstractpub_date
1989-03-13 00:00:00pages
164-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80213-3journal_volume
245pub_type
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