Biochemical evidence for histidine oxidation in photosystem II depleted of the Mn-cluster for O2-evolution.

abstract：:Circular dichroism (CD) spectra have been obtained from several variants of green fluorescent protein: blue fluorescent protein (BFP), enhanced cyan fluorescent protein (CFP), enhanced green fluorescent protein (GFP), enhanced yellow fluorescent protein (YFP), all from Aequorea victoria, and the red fluorescent protei...

journal_title：FEBS letters

authors： Visser NV,Hink MA,Borst JW,van der Krogt GN,Visser AJ

更新日期：2002-06-19 00:00:00

abstract：:Revised assignments for the bilayer helices of the beta-, gamma- and delta-subunits of the acetylcholine receptor are presented. A new feature of the model is extensive charge matching between the polar groups of the ion channel elements of different subunits. ...

journal_title：FEBS letters

authors： Kosower EM

更新日期：1984-06-25 00:00:00

abstract：:The isolation of a novel biologically active peptide, designated galanin, is described. The peptide was discovered by the detection of its C-terminal amide structure in porcine intestinal extract using a chemical method. It was found that galanin consists of 29 amino acids and the complete amino acid sequence is: Gly-...

journal_title：FEBS letters

authors： Tatemoto K,Rökaeus A,Jörnvall H,McDonald TJ,Mutt V

更新日期：1983-11-28 00:00:00

abstract：:Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that ...

journal_title：FEBS letters

authors： Kim HL,Park MB,Park YS

更新日期：2011-10-03 00:00:00

abstract：:Previously, we found that intracellular reactive oxygen species (ROS) affect photomorphogenesis in Neurospora crassa. In this study, we investigated the physiological roles of ROS in the response to light and found that the exposure of mycelia to air was important for the light-induced carotenogenesis. Mycelia treated...

journal_title：FEBS letters

authors： Iigusa H,Yoshida Y,Hasunuma K

更新日期：2005-07-18 00:00:00

abstract：:Mitochondrial creatine kinase (MiCK) occurs primarily as an octameric form localized in the mitochondrial intermembrane compartment in vertebrate tissues and echinoderm spermatozoa (both deuterostome groups). The octameric quaternary structure is thought to play important functional and enzyme targeting roles. We have...

journal_title：FEBS letters

authors： Ellington WR,Roux K,Pineda AO Jr

更新日期：1998-03-20 00:00:00

abstract：:One type of covalent linkages connecting lignin and hemicellulose in plant cell walls is the ester linkage between 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols. An enzyme that could hydrolyze such linkages, named glucuronoyl esterase, occurs in the cellulolytic system of the wood-rotting fungus S...

journal_title：FEBS letters

authors： Li XL,Spániková S,de Vries RP,Biely P

更新日期：2007-08-21 00:00:00

abstract：:Dystrophin is the recently discovered defective gene product in Duchenne and Becker muscular dystrophy (DMD and BMD). Dystrophin transcripts have been amplified and identified in diagnostic needle muscle biopsy samples using the polymerase chain reaction (PCR) procedure. Using 5'- and 3'-primers, dystrophin transcript...

journal_title：FEBS letters

authors： Muntoni F,Strong PN

更新日期：1989-07-31 00:00:00

abstract：:Since the discovery of the new members of the UCP (uncoupling protein) family, UCP2 and UCP3, very few studies have dealt with the regulation of their expression. Bearing this in mind, administration of a single intravenous injection of TNF-alpha (100 microg/kg body weight) to rats resulted in a significant increase i...

journal_title：FEBS letters

authors： Busquets S,Sanchís D,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

更新日期：1998-12-04 00:00:00

abstract：:Numerous biological proteins exhibit intrinsic disorder at their termini, which are associated with multifarious functional roles. Here, we show the surprising result that an increased percentage of terminal short transiently disordered regions with enhanced flexibility (TstDREF) is associated with accelerated folding...

journal_title：FEBS letters

authors： Mallik S,Ray T,Kundu S

更新日期：2017-07-01 00:00:00

abstract：:Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...

journal_title：FEBS letters

authors： Bittel C,Tabares LC,Armesto M,Carrillo N,Cortez N

更新日期：2003-10-23 00:00:00

abstract：:Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...

journal_title：FEBS letters

authors： Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

更新日期：2006-05-15 00:00:00

abstract：:Oligonucleotide-peptide conjugates have several applications, including their potential use as improved antisense agents for interfering with the RNA function within cells. In order to provide robust and generally applicable conjugation chemistry, we developed a novel approach of fragment coupling of pre-synthesized p...

journal_title：FEBS letters

authors： Zubin EM,Romanova EA,Volkov EM,Tashlitsky VN,Korshunova GA,Shabarova ZA,Oretskaya TS

更新日期：1999-07-30 00:00:00

abstract：:Organisms using the thiosulfate-oxidizing Sox enzyme system fall into two groups: group 1 forms sulfur globules as intermediates (Allochromatium vinosum), group 2 does not (Paracoccus pantotrophus). While several components of their Sox systems are quite similar, i.e. the proteins SoxXA, SoxYZ and SoxB, they differ by...

journal_title：FEBS letters

authors： Welte C,Hafner S,Krätzer C,Quentmeier A,Friedrich CG,Dahl C

更新日期：2009-04-17 00:00:00

abstract：:Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

journal_title：FEBS letters

authors： Kairane C,Zilmer M,Mutt V,Sillard R

更新日期：1994-05-23 00:00:00

abstract：:Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...

journal_title：FEBS letters

authors： Kwan DH,Jin Y,Jiang J,Chen HM,Kötzler MP,Overkleeft HS,Davies GJ,Withers SG

更新日期：2016-02-01 00:00:00

abstract：:Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...

journal_title：FEBS letters

authors： McGregor GP,Hartel R,Fehmann HC,Lankat-Buttgereit B,Göke B,Göke R,Voigt K

更新日期：1992-11-09 00:00:00

abstract：:Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the...

journal_title：FEBS letters

authors： Amler E,Jasińska R,Drahota Z,Zborowski J

更新日期：1990-10-01 00:00:00

abstract：:Data from a number of model systems support a role for proteolysis in apoptotic cell death. Using immature rat thymocytes, we demonstrate that the inhibitors N-tosyl-L-lysyl chloromethylketone (TLCK) and N-tosyl-L-phenylalanyl chloromethylketone (TPCK) have very different effects on apoptosis. TLCK inhibits apoptosis ...

journal_title：FEBS letters

authors： Fearnhead HO,Rivett AJ,Dinsdale D,Cohen GM

更新日期：1995-01-09 00:00:00

abstract：:The transport of proteins from the cytoplasm into the nucleus is a multistep process. The nuclear localization sequence (NLS) of a transport substrate associates with the heterodimeric NLS-receptor which binds to a subset of proteins of the nuclear pore complex (NPC). Translocation through the NPC is energy-dependent ...

journal_title：FEBS letters

authors： Schlenstedt G

更新日期：1996-06-24 00:00:00

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16-F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two specie...

journal_title：FEBS letters

authors： Moin K,Rozhin J,McKernan TB,Sanders VJ,Fong D,Honn KV,Sloane BF

更新日期：1989-02-13 00:00:00

abstract：:Cell stimulation raised the cytosolic free Ca2+ concentration, [Ca(2+]i and induces activation of Na+/H+ exchange which raises the cytosolic pH, pHi. Recent studies have addressed the question whether Na+/H+ exchange plays a role in Ca(2+) influx and, specifically, whether a rise in pHi alone suffices to open Ca(2+) c...

journal_title：FEBS letters

authors： Siffert W,Akkerman JW

更新日期：1989-12-18 00:00:00

abstract：:Contact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or Rho...

journal_title：FEBS letters

authors： Anear E,Parish RW

更新日期：2012-05-07 00:00:00

abstract：:We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...

journal_title：FEBS letters

authors： Hwang MG,Chung IK,Kang BG,Cho MH

更新日期：2001-08-10 00:00:00

abstract：:The addition of hepatocyte growth factor (HGF) to rat hepatocytes in primary culture resulted in the formation of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and 1,2-diacylglycerol (DG) by a phosphoinositide-specific phospholipase C (PI-PLC). DG showed a biphasic increase; the first phase, corresponding with the peak ...

journal_title：FEBS letters

authors： Osada S,Nakashima S,Saji S,Nakamura T,Nozawa Y

更新日期：1992-02-10 00:00:00

abstract：:It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...

journal_title：FEBS letters

authors： Lin Y,Chen H,Hu Z,Mao Y,Xu X,Zhu Y,Xu X,Wu J,Li S,Mao Q,Zheng X,Xie L

更新日期：2013-08-02 00:00:00

abstract：:Globular protein thermostability is characterized the cold denaturation, maximal stability (Tms) and heat denaturation temperatures. For mesophilic globular proteins, Tms typically ranges from -25 degrees C to +35 degrees C. We show that the indirect estimate of Tms from calorimetry and the direct estimate from chemic...

journal_title：FEBS letters

authors： Ganesh C,Eswar N,Srivastava S,Ramakrishnan C,Varadarajan R

更新日期：1999-07-02 00:00:00

abstract：:A human placenta cDNA expression library was screened for genes inducing flat reversion when transfected into a v-K-ras-transformed NIH3T3 cell line, DT. One such gene was found to encode a Kunitz-type serine protease inhibitor, tissue factor pathway inhibitor-2 (TFPI-2). While the TFPI-2 mRNA can be detected in norma...

journal_title：FEBS letters

authors： Izumi H,Takahashi C,Oh J,Noda M

更新日期：2000-09-08 00:00:00

abstract：:Thrombin has been shown to inhibit skeletal muscle differentiation. However, the mechanisms by which thrombin represses myogenesis remain unknown. Since the thrombin receptor couples to G(i), G(q/11) and G(12), we examined which subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (Galpha(i), Galp...

journal_title：FEBS letters

authors： Nagao M,Kaziro Y,Itoh H

更新日期：2000-04-28 00:00:00