Abstract:
:Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first stable cell-line found to express the PPT-I gene and provides an opportunity for investigating PPT-I gene expression and tachykinin biosynthesis. RIN5mF cells are a model for the pancreatic beta-cell, which is not known to exhibit PPT-I gene expression which may, therefore, be a feature of the transformed state of these cells. These data may imply that the tachykinins are important in pancreatic islet embryogenesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
McGregor GP,Hartel R,Fehmann HC,Lankat-Buttgereit B,Göke B,Göke R,Voigt Kdoi
10.1016/0014-5793(92)80932-7keywords:
subject
Has Abstractpub_date
1992-11-09 00:00:00pages
187-91issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(92)80932-7journal_volume
312pub_type
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