Abstract:
:A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazone back to epsilon-N-trimethyllysine by hydrogenolysis in a Parr bomb. Analyses on TLC and in four different ion exchange chromatographic systems show the appearance of a ninhydrin positive product having the same Rf and the same retention time as epsilon-N-trimethyllysine; it contains more than 85% of the radioactivity of the 2,4-dinitrophenylhydrazone of the keto acid.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Villanueva VR,Lederer Edoi
10.1016/0014-5793(75)80832-5keywords:
subject
Has Abstractpub_date
1975-04-01 00:00:00pages
308-11issue
2eissn
0014-5793issn
1873-3468journal_volume
52pub_type
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