Abstract:
:Complementary (c) DNAs encoding a glycine receptor (GlyR) isomer were cloned from libraries constructed in lambda ZAPII with poly (A)+ RNA of neonatal rat spinal cord. Northern blot analysis revealed that RNA hybridized to the cloned cDNA is detectable only for a period of late embryonic/early postnatal stage of the spinal cord. Moreover, other central nervous tissues, such as hippocampus and cerebral cortex, in the infant rats are also rich in this message. The 'neonatal (N) GlyR' has 71% homology to that of another GlyR isoform in which adult rad cord is rich (AGlyR). Injection of a single RNA transcribed from the NGlyr-cDNA into Xenopus oocyte induced functional formation of glycine-gated Cl- channels, however, its pharmacological property differed from that of AGlyR.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Akagi H,Hirai K,Hishinuma Fdoi
10.1016/0014-5793(91)80383-esubject
Has Abstractpub_date
1991-04-09 00:00:00pages
160-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80383-Ejournal_volume
281pub_type
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