Studies on the maintenance of cytochromes P-450 and b5, monooxygenases and cytochrome reductases in primary cultures of rat hepatocytes.

abstract：:Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...

journal_title：FEBS letters

authors： McGregor GP,Hartel R,Fehmann HC,Lankat-Buttgereit B,Göke B,Göke R,Voigt K

更新日期：1992-11-09 00:00:00

abstract：:The polytopic transmembrane protein, Niemann-Pick C1-Like 1 (NPC1L1), is enriched in the apical membrane of small intestine absorptive enterocytes where it mediates extracellular sterol transport across the brush border membrane. It is essential for intestinal sterol absorption and is the molecular target of ezetimibe...

journal_title：FEBS letters

authors： Betters JL,Yu L

更新日期：2010-07-02 00:00:00

abstract：:Cytochrome bd is a bacterial respiratory oxidase carrying three hemes but no copper. We show that nitric oxide (NO) reacts with the intermediate F of cytochrome bd from Azotobacter vinelandii: (i) with a 1:1 stoichiometry, (ii) rapidly (k=1.2 +/- 0.1 x 10(5)M(-1)s(-1) at 20 degrees C), and (iii) yielding the oxidized ...

journal_title：FEBS letters

authors： Borisov VB,Forte E,Sarti P,Brunori M,Konstantinov AA,Giuffrè A

更新日期：2006-09-04 00:00:00

abstract：:Chemical modification of lysine residues in glucose oxidase was carried out using citraconic anhydride. Modification brought about changes in the kinetic properties of the enzyme as evident by substantial lowering of V(max) and K(m). Enhancement of tryptophan fluorescence was observed with a dramatic change in its pH ...

journal_title：FEBS letters

authors： Hosseinkhani S,Ranjbar B,Naderi-Manesh H,Nemat-Gorgani M

更新日期：2004-03-12 00:00:00

abstract：:Proteins with a CyaB, thiamine triphosphatase domain (CYTH domain) may play a central role at the interface between nucleotide and polyphosphate metabolism. One of the plant CYTH domain-containing proteins from Brachypodium distachyon, BdTTM3, is annotated in NCBI databases as an 'adenylyl cyclase (AC)' or a 'triphosp...

journal_title：FEBS letters

authors： Świeżawska B,Duszyn M,Kwiatkowski M,Jaworski K,Pawełek A,Szmidt-Jaworska A

更新日期：2020-03-01 00:00:00

abstract：:We explored the interactive effects of endothelial nitric oxide synthase (eNOS) genotypes and cigarette smoking on protein levels and enzyme activity in 33 postpartum placentas. Whilst the eNOS protein levels were lower in the rare allele (0.48+/-0.11, n=9 vs. 1. 05+/-0.10, n=24, P<0.01), the eNOS enzyme activity was ...

journal_title：FEBS letters

authors： Wang XL,Sim AS,Wang MX,Murrell GA,Trudinger B,Wang J

更新日期：2000-04-07 00:00:00

abstract：:The fluorescence intensity of the Ca2+,Mg2(+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 microM calcium. The observed enhancement indicates that the fluorophore, ...

journal_title：FEBS letters

authors： Bailin G,Huang JR

更新日期：1990-01-01 00:00:00

abstract：:A novel allergenic member of the family of Ca(2+)-binding proteins has been cloned from olive tree pollen. The isolated DNA codes for a protein of 171 amino acid residues, which displays four EF-hand sequence motifs. The encoded protein was overproduced in Escherichia coli and purified. The protein (18¿ omitted¿795 Da...

journal_title：FEBS letters

authors： Ledesma A,Villalba M,Rodríguez R

更新日期：2000-01-21 00:00:00

abstract：:ISG20 is an interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease superfamily. We have solved the crystal structure of human ISG20 complexed with two Mn2+ ions and uridine 5'...

journal_title：FEBS letters

authors： Horio T,Murai M,Inoue T,Hamasaki T,Tanaka T,Ohgi T

更新日期：2004-11-05 00:00:00

abstract：:As a result of an experimental confluence between carcinogenesis and oxidative phosphorylation, we published in 1971 'A Unitary Hypothesis for Carcinogenesis' [(1971) J. Antibiot. Tokyo 24, 405-417]. According to this hypothesis, damaged mitochondria release mitochondrial genetic material which like that from an oncog...

journal_title：FEBS letters

authors： Hadler HI

更新日期：1989-10-09 00:00:00

abstract：:Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid and glucose homeostasis. Here we show that FXR is expressed and functional, as assessed by target gene expression analysis, in human islets and beta-cell lines. FXR is predominantly cytosolic-localized in the ...

journal_title：FEBS letters

authors： Popescu IR,Helleboid-Chapman A,Lucas A,Vandewalle B,Dumont J,Bouchaert E,Derudas B,Kerr-Conte J,Caron S,Pattou F,Staels B

更新日期：2010-07-02 00:00:00

abstract：:Stability of green fluorescent protein (GFP) is sometimes important for a proper practical application of this protein. Random mutagenesis and targeted mutagenesis have been used to create better-folded variants of GFP, including recently reported extra-superfolder GFP. Our aim was to determine the crystal structure o...

journal_title：FEBS letters

authors： Choi JY,Jang TH,Park HH

更新日期：2017-01-01 00:00:00

abstract：:31P-NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glyco...

journal_title：FEBS letters

authors： Egan W,Barile M,Rottem S

更新日期：1986-08-18 00:00:00

abstract：:Secreted proteins are indispensable for the development and differentiation of multicellular organisms. Cloning and characterization of novel or hypothetical genes encoding these proteins are therefore inviting great incentives. Using bioinformatics tools and experimental approaches, we isolated and characterized a hu...

journal_title：FEBS letters

authors： Zeng LC,Liu F,Zhang X,Zhu ZD,Wang ZQ,Han ZG,Ma WJ

更新日期：2004-07-30 00:00:00

abstract：:The lipid A structure of the Gram-negative bacterium Helicobacter mustelae, a ferret gastric pathogen responsible for the onset of gastric diseases in its host, was investigated. Two variant lipid A structures were found in the same strain. One structure contained a bisphosphorylated beta-(1-->6)-linked D-glucosamine ...

journal_title：FEBS letters

authors： Thérisod H,Monteiro MA,Perry MB,Caroff M

更新日期：2001-06-15 00:00:00

abstract：:The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

journal_title：FEBS letters

authors： Ellermann J,Kobelt A,Pfaltz A,Thauer RK

更新日期：1987-08-17 00:00:00

abstract：:The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...

journal_title：FEBS letters

authors： Hakoshima T

更新日期：2018-02-01 00:00:00

abstract：:The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mi...

journal_title：FEBS letters

authors： Chloupková M,Reaves SK,LeBard LM,Koeller DM

更新日期：2004-07-02 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

journal_title：FEBS letters

authors： Hecker M,Walsh DT,Vane JR

更新日期：1991-12-09 00:00:00

abstract：:The cellulases cellobiohydrolase 1 (CBH 1) and endoglucanase 1 (EG 1) from the fungus Trichoderma reesei are closely related with 40% sequence identity and very similar in structure. In CBH 1 the active site is enclosed by long loops and some antiparallel beta-strands forming a 40 A long tunnel, whereas in EG 1 part o...

journal_title：FEBS letters

authors： Henriksson H,Ståhlberg J,Isaksson R,Pettersson G

更新日期：1996-07-29 00:00:00

abstract：:Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...

journal_title：FEBS letters

authors： Roth B,Schwendimann B,Hughes GJ,Tzartos SJ,Barkas T

更新日期：1987-08-31 00:00:00

abstract：:Modulation of intracellular guanosine 3',5'-bispyrophosphate ((p)ppGpp) level, the effector of the stringent response, is crucial for survival as well as optimal growth of prokaryotes and, thus, for bacterial pathogenesis and dormancy. In Mycobacterium tuberculosis (Mtb), (p)ppGpp synthesis and degradation are carried...

journal_title：FEBS letters

authors： Singal B,Balakrishna AM,Nartey W,Manimekalai MSS,Jeyakanthan J,Grüber G

更新日期：2017-08-01 00:00:00

abstract：:Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was...

journal_title：FEBS letters

authors： Miyamae T,Fukushima N,Misu Y,Ueda H

更新日期：1993-11-01 00:00:00

abstract：:NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...

journal_title：FEBS letters

authors： Drincovich MF,Casati P,Andreo CS

更新日期：2001-02-09 00:00:00

abstract：:In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brai...

journal_title：FEBS letters

authors： Seebahn A,Rose M,Enz R

更新日期：2008-07-09 00:00:00

abstract：:Prohormone convertases (PCs) 1 and 2 are thought to mediate the proteolytic cleavage of many peptide precursors. Endogenous inhibitors of both PC1 and PC2 have now been identified; the 7B2 protein is a nanomolar inhibitor of PC2, while the novel protein proSAAS was recently reported to be a micromolar inhibitor of PC1...

journal_title：FEBS letters

authors： Cameron A,Fortenberry Y,Lindberg I

更新日期：2000-05-12 00:00:00

abstract：:We recently showed that some of the enzymes underpinning cellulose solubilization by Ruminococcus albus 8 lack the conventional type of dockerin module characteristic of cellulosomal proteins and instead, bear an "X" domain of unknown function at their C-termini. We have now subcloned and expressed six X domains and s...

journal_title：FEBS letters

authors： Xu Q,Morrison M,Nelson KE,Bayer EA,Atamna N,Lamed R

更新日期：2004-05-21 00:00:00

abstract：:Over the past several years, the study of a hereditary tumor syndrome, tuberous sclerosis complex (TSC), has shed light on the regulation of cellular proliferation and growth. TSC is an autosomal dominant disorder that is due to inactivating mutations in TSC1 or TSC2 and characterized by benign tumors (hamartomas) inv...

journal_title：FEBS letters

authors： Han JM,Sahin M

更新日期：2011-04-06 00:00:00

abstract：:A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effe...

journal_title：FEBS letters

authors： Schofield PN,Granerus M,Lee A,Ektröm TJ,Engström W

更新日期：1992-02-24 00:00:00

abstract：:Blood flukes of the genus Schistosoma currently infect millions of people in tropical and subtropical countries. An enzyme playing a major role in hemoglobin (Hb) degradation by Schistosoma mansoni has been cloned and shown to be highly similar to the human cathepsin D aspartyl proteinase, although presenting a distin...

journal_title：FEBS letters

authors： Silva FP Jr,Ribeiro F,Katz N,Giovanni-De-Simone S

更新日期：2002-03-13 00:00:00