Exploring the subsite specificity of Schistosoma mansoni aspartyl hemoglobinase through comparative molecular modelling.

abstract：:Acylphosphatase (AcP) activity in prokaryotes was classically attributed to some aspecific acid phosphatases. We identified an open reading frame for a putative AcP in the b0968 Escherichia coli gene and purified the recombinant enzyme after checking by RT-PCR that it was indeed expressed. EcoAcP has a predicted typic...

journal_title：FEBS letters

authors： Ramazzotti M,Parrini C,Stefani M,Manao G,Degl'Innocenti D

更新日期：2006-12-22 00:00:00

abstract：:Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO(3)(-)/H(+) antiporter critical for nitrate transpo...

journal_title：FEBS letters

authors： Zifarelli G,Pusch M

更新日期：2010-05-17 00:00:00

abstract：:A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...

journal_title：FEBS letters

authors： Powers MV,Workman P

更新日期：2007-07-31 00:00:00

abstract：:Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are...

journal_title：FEBS letters

authors： Barlach S,Schumann W

更新日期：1983-06-27 00:00:00

abstract：:The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

journal_title：FEBS letters

authors： Durchschlag H,Zipper P

更新日期：1988-09-12 00:00:00

abstract：:New polypeptide components have been isolated from Dendroaspis angusticeps venom using chromatography. Two polypeptides containing 59 and 57 amino acids, called 'DaE1' and 'DaE2' respectively, have been purified to homogeneity and fully sequenced. Spectrometric analysis yielded masses of 6631.5 and 6389.0 Da, respecti...

journal_title：FEBS letters

authors： Tytgat J,Vandenberghe I,Ulens C,Van Beeumen J

更新日期：2001-03-02 00:00:00

abstract：:A new mutant alpha-1-antichymotrypsin (variant ACT) was found by direct sequencing and PCR-single strand conformation polymorphism (PCR-SSCP). This variant ACT was a point mutation of exon V of ACT, with the substitution of Met by Val. Four out of six individuals with this variant ACT had occlusive-cerebrovascular dis...

journal_title：FEBS letters

authors： Tsuda M,Sei Y,Yamamura M,Yamamoto M,Shinohara Y

更新日期：1992-06-08 00:00:00

abstract：:The fluorescence intensity and anisotropy decays of the intrinsic tryptophan emission from six Fe/S proteins (ranging from the very simplest ones to enzyme complexes containing one, two or more Trp residues) were measured. All proteins were examined in the reduced and the oxidized state. In either redox state each pro...

journal_title：FEBS letters

authors： Dorovska-Taran V,van Hoek A,Link TA,Visser AJ,Hagen WR

更新日期：1994-07-18 00:00:00

abstract：:Recombinant expression of the large alternansucrase (2057 amino acids) was hindered in E. coli due to poor enzyme solubility and protein degradation. The effects of deletions of the alternansucrase C-terminal CW-like and APY repeated motifs on enzyme solubility and specificity were investigated. A truncated variant de...

journal_title：FEBS letters

authors： Joucla G,Pizzut S,Monsan P,Remaud-Simeon M

更新日期：2006-02-06 00:00:00

abstract：:Analysis of glutamic acid decarboxylase (GDC) (EC 4.1.1.15) from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...

journal_title：FEBS letters

authors： van der Meer RA,Groen BW,Duine JA

更新日期：1989-03-27 00:00:00

abstract：:Streptococci are a broad group of Gram-positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens hav...

journal_title：FEBS letters

authors： Syed S,Viazmina L,Mager R,Meri S,Haapasalo K

更新日期：2020-08-01 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP c...

journal_title：FEBS letters

authors： Jarmuszkiewicz W,Antos N,Swida A,Czarna M,Sluse FE

更新日期：2004-07-02 00:00:00

abstract：:The NFkappaB family of transcription factors is regulated by inhibitory IkappaB proteins. A diversity of stimuli leads to the phosphorylation and subsequent degradation of IkappaB, releasing NFkappaB to act on its target genes. Calmodulin (CaM) is a key regulator of numerous cellular processes and is the predominant i...

journal_title：FEBS letters

authors： Hughes K,Antonsson A,Grundstrøm T

更新日期：1998-12-11 00:00:00

abstract：:Rhodamine 6G (3 microM) effectively inhibited delta pH-driven ATP synthesis in Methanobacterium thermoautotrophicum while delta pNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg(2+)-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detach...

journal_title：FEBS letters

authors： Smigán P,Majerník A,Greksák M

更新日期：1994-06-27 00:00:00

abstract：:Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...

journal_title：FEBS letters

authors： Bartling B,Hilgefort C,Friedrich I,Silber RE,Simm A

更新日期：2003-12-18 00:00:00

abstract：:Activation of T lymphocytes results in an increase in intracellular Ca2+ due in large part to influx of extracellular Ca2+. Using the patch clamp technique, an inward current in Jurkat T lymphocytes was observed upon depolarization from a holding potential of -90 mV but not from -60 mV. This whole-cell current was ins...

journal_title：FEBS letters

authors： Densmore JJ,Szabo G,Gray LS

更新日期：1992-11-09 00:00:00

abstract：:The expression of rat brain voltage-sensitive Na+ channel mRNAs in Schwann cells was examined using in situ hybridization cytochemistry and RT-PCR. The mRNAs of rat brain Na+ channel subtype II and III, but not subtype I, were detected in cultured Schwann cells from sciatic nerve and in intact sciatic nerve, which con...

journal_title：FEBS letters

authors： Oh Y,Black JA,Waxman SG

更新日期：1994-08-22 00:00:00

abstract：:Plastidic type I signal peptidase 1 (Plsp1) is necessary for proper chloroplast development although its mode of action is not completely understood. Here, we demonstrate by immunoblotting and electron microscopy immunolocalization that Plsp1 is evenly distributed in the envelope and thylakoids of developing chloropla...

journal_title：FEBS letters

authors： Shipman RL,Inoue K

更新日期：2009-03-04 00:00:00

abstract：:This article presents an overview of the simulation studies of the behaviour of multihaem cytochromes using theoretical/computational methodologies, with an emphasis on cytochrome c(3). It starts with the first studies using rigid molecules and continuum electrostatic models, where protonation and redox events were tr...

journal_title：FEBS letters

authors： Soares CM,Baptista AM

更新日期：2012-03-09 00:00:00

abstract：:Interrogation of the Human Genome data for sequences related to the von Willebrand factor A-domain module identified a previously unreported 4.1 kb full-length cDNA that is predicted to encode a new member of the collagen superfamily of extracellular matrix proteins, collagen XXI. The domain organization of collagen X...

journal_title：FEBS letters

authors： Fitzgerald J,Bateman JF

更新日期：2001-09-14 00:00:00

abstract：:Genetically obese (ob/ob) mice develop a marked insulin resistance in brown adipose tissue soon after weaning, and this is paralleled by a fall in the acute activation of the mitochondrial proton conductance pathway in the tissue on cold exposure. Treatment of ob/ob mice with ciglitazone, a new oral hypoglycaemic, led...

journal_title：FEBS letters

authors： Mercer SW,Trayhurn P

更新日期：1986-01-20 00:00:00

abstract：:Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by sit...

journal_title：FEBS letters

authors： Starkova NN,Koroleva EP,Rumsh LD,Ginodman LM,Rotanova TV

更新日期：1998-01-30 00:00:00

abstract：:In order to better define the structural elements involved in allosteric signalling, wild-type DnaK and three deletion mutants of the peptide binding domain have been characterized by biophysical (steady-state and time-resolved fluorescence) and biochemical methods. In the presence of ATP the chemical environment of t...

journal_title：FEBS letters

authors： Moro F,Fernández V,Muga A

更新日期：2003-01-02 00:00:00

abstract：:Accumulating evidence has indicated that microRNAs (miRNAs) act as critical epigenetic regulators in tumor carcinogenesis. Here, we report that miR-199a-3p was significantly upregulated in gastric cancer (GC) cell lines and tissues. Functional studies demonstrated that miR-199a-3p dramatically increased cell prolifera...

journal_title：FEBS letters

authors： Wang Z,Ma X,Cai Q,Wang X,Yu B,Cai Q,liu B,Zhu Z,Li C

更新日期：2014-11-28 00:00:00

abstract：:Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produ...

journal_title：FEBS letters

authors： Rosario Fernández M,Jörnvall H,Moreno A,Kaiser R,Parés X

更新日期：1993-08-16 00:00:00

abstract：:Automatic design is based on computational modeling and optimization methods to provide prototype designs to targeted problems in an unsupervised manner. For biological circuits, we need to produce quantitative predictions of cell behavior for a given genotype as consequence of the different molecular interactions. Au...

journal_title：FEBS letters

authors： Rodrigo G,Carrera J,Landrain TE,Jaramillo A

更新日期：2012-07-16 00:00:00

abstract：:When the eicosapentaenoic acid (EPA)-deficient mutant strain IK-1Delta8 of the marine EPA-producing Shewanella marinintestina IK-1 was treated with various concentrations of hydrogen peroxide (H(2)O(2)), its colony-forming ability decreased more than that of the wild type. Protein carbonylation, induced by treating ce...

journal_title：FEBS letters

authors： Nishida T,Morita N,Yano Y,Orikasa Y,Okuyama H

更新日期：2007-09-04 00:00:00

abstract：:Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. ...

journal_title：FEBS letters

authors： Sugawara K,Fujikawa M,Yoshida M

更新日期：2013-11-29 00:00:00

abstract：:We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...

journal_title：FEBS letters

authors： Birkett CR,Foster KE,Johnson L,Gull K

更新日期：1985-08-05 00:00:00

abstract：:Specific binding sites for 3,3'-T2 can be detected in swollen and osmotically treated mitochondria (OTM) from normal and hypothyroid rat liver. In hypothyroid animals, maximal values of binding were obtained at 0 degrees C while values were lower at 37 degrees C and no specific binding could be observed at 60 degrees ...

journal_title：FEBS letters

authors： Lanni A,Moreno M,Horst C,Lombardi A,Goglia F

更新日期：1994-09-05 00:00:00