Abstract:
:Automatic design is based on computational modeling and optimization methods to provide prototype designs to targeted problems in an unsupervised manner. For biological circuits, we need to produce quantitative predictions of cell behavior for a given genotype as consequence of the different molecular interactions. Automatic design techniques aim at solving the inverse problem of finding the sequences of nucleotides that better fit a targeted behavior. In the post-genomic era, our molecular knowledge and modeling capabilities have allowed to start using such methodologies with success. Herein, we describe how the emergence of this new type of tools could enable novel synthetic biology applications. We highlight the essential elements to develop automatic design procedures for synthetic biology pointing out their advantages and bottlenecks. We discuss in detail the experimental difficulties to overcome in the in vivo implementation of designed networks. The use of automatic design to engineer biological networks is starting to emerge as a new technique to perform synthetic biology, which should not be neglected in the future.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rodrigo G,Carrera J,Landrain TE,Jaramillo Adoi
10.1016/j.febslet.2012.02.031subject
Has Abstractpub_date
2012-07-16 00:00:00pages
2037-42issue
15eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00154-8journal_volume
586pub_type
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