A voltage-gated calcium channel is linked to the antigen receptor in Jurkat T lymphocytes.

abstract：:Automatic design is based on computational modeling and optimization methods to provide prototype designs to targeted problems in an unsupervised manner. For biological circuits, we need to produce quantitative predictions of cell behavior for a given genotype as consequence of the different molecular interactions. Au...

journal_title：FEBS letters

authors： Rodrigo G,Carrera J,Landrain TE,Jaramillo A

更新日期：2012-07-16 00:00:00

abstract：:Stimulation of mast cells through their high affinity IgE receptor (FcεRI) leads to the secretion of pre- and neoformed soluble mediators through vesicular carriers. This process is highly regulated in order to adapt the secretion of these potentially dangerous factors to the physiological needs. This regulation requi...

journal_title：FEBS letters

authors： Benhamou M,Blank U

更新日期：2010-12-15 00:00:00

abstract：:The gene of ecotin, an E. coli proteinase inhibitor, was cloned, and by site-directed mutagenesis the active site residue of the protein, Met84, was mutated to Lys, Arg and Leu. The recombinant wild-type and mutant inhibitors were overexpressed in E. coli, purified to homogeneity and their inhibitory effects on trypsi...

journal_title：FEBS letters

authors： Pál G,Sprengel G,Patthy A,Gráf L

更新日期：1994-03-28 00:00:00

abstract：:Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

journal_title：FEBS letters

authors： Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

更新日期：2007-01-09 00:00:00

abstract：:Glycine amide (GlyAd), a typically amidated amino acid, is a versatile additive that suppresses protein aggregation during refolding, heat treatment, and crystallization. In spite of its effectiveness, the exact mechanism by which GlyAd suppresses protein aggregation remains to be elucidated. Here, we show the crystal...

journal_title：FEBS letters

authors： Ito L,Shiraki K,Makino M,Hasegawa K,Kumasaka T

更新日期：2011-02-04 00:00:00

abstract：:Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose ...

journal_title：FEBS letters

authors： King IA,Magee AI,Rees DA,Buxton RS

更新日期：1991-07-29 00:00:00

abstract：:Because A2 adenosine receptor activation stimulates adenylate cyclase and cyclic AMP induces 5'-nucleotidase expression in rat mesangial cells, we examined the effect of adenosine and its analogs on 5'-nucleotidase activity in these cells. A2 adenosine receptors were characterized using [3H]5'-N-ethylcarboxamidoadenos...

journal_title：FEBS letters

authors： Stefanovic V,Vlahovic P,Savic V,Ardaillou N,Ardaillou R

更新日期：1993-09-27 00:00:00

abstract：:alpha 1 Antitrypsin (alpha 1AT) is the main physiological inhibitor of neutrophil elastase, a serine protease which has been implicated in tissue degradation at inflammatory sites. We report here that the connective tissue metalloproteinase, stromelysin, cleaved alpha 1AT (54 kDa), producing fragments of approximately...

journal_title：FEBS letters

authors： Winyard PG,Zhang Z,Chidwick K,Blake DR,Carrell RW,Murphy G

更新日期：1991-02-11 00:00:00

abstract：:Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...

journal_title：FEBS letters

authors： Kitlar T,Morrison AI,Kinne R,Deutscher J

更新日期：1988-07-04 00:00:00

abstract：:The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma ...

journal_title：FEBS letters

authors： Ciruela F,McIlhinney RA

更新日期：1997-11-24 00:00:00

abstract：:X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

journal_title：FEBS letters

authors： Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

更新日期：2012-06-04 00:00:00

abstract：:Tumor necrosis factor receptor associated factor 6 (TRAF6) is an important signaling molecule involved in a diverse array of physiological processes. It has been proposed that TRAF6, a RING finger-containing protein, acts as a ubiquitin ligase (E3) and a target for Lys-63 linked polyubiquitination mediated by Ubc13-Ue...

journal_title：FEBS letters

authors： Wooff J,Pastushok L,Hanna M,Fu Y,Xiao W

更新日期：2004-05-21 00:00:00

abstract：:The surprising result that the spanning domain causes retention of proteins in the Golgi stack poses the question as to the actual mechanism. Here we present a simple model that might have general applicability. ...

journal_title：FEBS letters

authors： Nilsson T,Slusarewicz P,Hoe MH,Warren G

更新日期：1993-09-06 00:00:00

abstract：:Aminopeptidase yscXVI was purified from the yeast Saccharomyces cerevisiae. By SDS-PAGE the enzyme has a molecular weight of 45,000 Da, and in chromatofocusing, elution was observed at pH 6.2. The synthetic substrate cystinyl-4-nitroanilide (Km 22.5 microM, Vmax 12.9 mU/mg) is cleaved most efficiently in the pH range ...

journal_title：FEBS letters

authors： Tisljar U,Wolf DH

更新日期：1993-05-10 00:00:00

abstract：:In PC12 cells, cAMP stimulates the MAP kinase pathway by an unknown mechanism. Firstly, we examined the role of calcium ion mobilization and of protein kinase C in cAMP-stimulated MAP kinase activation. We show that cAMP stimulates p44mapk independently of these events. Secondly, we studied the role of B-Raf in this p...

journal_title：FEBS letters

authors： Peraldi P,Frödin M,Barnier JV,Calleja V,Scimeca JC,Filloux C,Calothy G,Van Obberghen E

更新日期：1995-01-09 00:00:00

abstract：:Chicken muscular dystrophy with abnormal muscle (AM) has been studied for more than 50 years, but the gene responsible for it remains unclear. Our previous studies narrowed down the AM candidate region to approximately 1Mbp of chicken chromosome 2q containing seven genes. In this study, we performed sequence compariso...

journal_title：FEBS letters

authors： Matsumoto H,Maruse H,Inaba Y,Yoshizawa K,Sasazaki S,Fujiwara A,Nishibori M,Nakamura A,Takeda S,Ichihara N,Kikuchi T,Mukai F,Mannen H

更新日期：2008-06-25 00:00:00

abstract：:Lysophosphatidic acid (LPA) exerts multiple biological functions through G protein-coupled receptors (EDG2/LPA(1), EDG4/LPA(2), and EDG7/LPA(3)) and is present in serum where it is associated with albumin. In this study we examined LPA activity in various biological fluids by measuring the LPA-induced increase in the ...

journal_title：FEBS letters

authors： Hama K,Bandoh K,Kakehi Y,Aoki J,Arai H

更新日期：2002-07-17 00:00:00

abstract：:As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-...

journal_title：FEBS letters

authors： Syomin BV,Kandror KV,Semakin AB,Tsuprun VL,Stepanov AS

更新日期：1993-06-01 00:00:00

abstract：:FT-IR analysis shows that treatment of F1ATPase with the inhibitors DCCD and Nbf-Cl, in the presence of saturating concentrations of ADP and AMP-PNP and in the absence of Mg2+, does not modify the secondary structure of the enzyme, but significantly modifies its compactness and thermal stability, although to different...

journal_title：FEBS letters

authors： Lippe G,Tanfani F,Di Pancrazio F,Contessi S,Bertoli E,Dabbeni-Sala F

更新日期：1998-08-07 00:00:00

abstract：:Yeast Golgi ecto-ATPase Ynd1p is an unusual type III membrane protein with the longest translocated N-terminus reported. Sequential deletion analysis reveals that translocation of this 500-residue-long hydrophilic domain across the membranes requires the C-terminal transmembrane domain of Ynd1p and its flanking region...

journal_title：FEBS letters

authors： Zhong X,Malhotra R,Guidotti G

更新日期：2005-10-24 00:00:00

abstract：:The range of delta-selectivity of linear and cyclic analogues of enkephalin in rat brain was found to be: [D-Pen2, L-Pen5] enkephalin (DPLPE) greater than [D-Pen2, D-Pen5] enkephalin (DPDPE) greater than [D-Thr2, Leu5] enkephalyl-Thr6 (DTLET) greater than [D-Ser2, Leu5] enkephalyl-Thr6 (DSLET). Saturation experiments ...

journal_title：FEBS letters

authors： Delay-Goyet P,Zajac JM,Rigaudy P,Foucaud B,Roques BP

更新日期：1985-04-22 00:00:00

abstract：:Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2Apro have been generated. One hybrid protein contains the poliovirus 2Apro sequence replacing the region of PE corresponding to amino acids 413-607. The other hybrid contains in addition the transforming growth factor sequence....

journal_title：FEBS letters

authors： Novoa I,Feduchi E,Carrasco L

更新日期：1994-11-21 00:00:00

abstract：:The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...

journal_title：FEBS letters

authors： Rosenblum JS,Nomanbhoy TK,Kozarich JW

更新日期：2013-06-27 00:00:00

abstract：:FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role ...

journal_title：FEBS letters

authors： Leung TW,Lin SS,Tsang AC,Tong CS,Ching JC,Leung WY,Gimlich R,Wong GG,Yao KM

更新日期：2001-10-19 00:00:00

abstract：:We have studied the modulation and pharmacological properties of two anion channels in T84 cells by recording single channel and transepithelial currents. One channel had an outwardly rectifying current-voltage I/V curve, was rarely active in cell-attached patches, and was unaffected by cAMP. The other channel had low...

journal_title：FEBS letters

authors： Tabcharani JA,Low W,Elie D,Hanrahan JW

更新日期：1990-09-17 00:00:00

abstract：:Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...

journal_title：FEBS letters

authors： Mahon MJ,Shimada M

更新日期：2005-01-31 00:00:00

abstract：:Mesenchyme forkhead-1 (MFH-1), a winged helix/forkhead transcription factor, is expressed in developing cartilaginous tissues, kidney and arch arteries, and is essential for the normal development of the axial skeleton and aortic arch formation of mice. To investigate the possible role of MFH-1 in osteogenesis and ost...

journal_title：FEBS letters

authors： Yang XL,Matsuura H,Fu Y,Sugiyama T,Miura N

更新日期：2000-03-17 00:00:00

abstract：:Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid into prostanoids which are involved in apoptosis and inflammation. Two distinct COXs have been identified: COX-1 which is constitutively expressed and COX-2 which is induced by different products such as tumor promoters or growth factors. Pre...

journal_title：FEBS letters

authors： Moalic S,Liagre B,Corbière C,Bianchi A,Dauça M,Bordji K,Beneytout JL

更新日期：2001-10-12 00:00:00

abstract：:The intestinal microbiota is essential for nutrient acquisition, immune development, and exclusion of invading pathogens. The upper respiratory tract (URT) microbiota is less well studied and does not appear to abide by many of the paradigms of the gastrointestinal tract. Decades of carriage studies in children have d...

journal_title：FEBS letters

authors： Schenck LP,Surette MG,Bowdish DM

更新日期：2016-11-01 00:00:00

abstract：:Using the yeast two hybrid system and overlay assays we identified a putative rholrac effector, citron, which interacts with the GTP-bound forms of rho and rac1, but not with cdc42. Extensive homologies to known proteins were not observed. This 183 kDa protein contains a C6H2 zinc finger, a PH domain, and a long coile...

journal_title：FEBS letters

authors： Madaule P,Furuyashiki T,Reid T,Ishizaki T,Watanabe G,Morii N,Narumiya S

更新日期：1995-12-18 00:00:00