Abstract:
:FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role of FoxM1 and its downstream regulatory targets, three stably transfected HeLa lines that display doxycycline (dox)-inducible FoxM1 expression were established. Over-expression of FoxM1 by dox induction facilitates growth recovery from serum starvation. Quantitation of cyclin B1 and D1 levels using flow cytometric, Western and Northern analyses reveals that elevated FoxM1 levels lead to stimulation of cyclin B1 but not cyclin D1 expression. Transient reporter assays in the dox-inducible lines and upon co-transfection with a constitutive FoxM1 expression plasmid suggest that FoxM1 can activate the cyclin B1 promoter.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Leung TW,Lin SS,Tsang AC,Tong CS,Ching JC,Leung WY,Gimlich R,Wong GG,Yao KMdoi
10.1016/s0014-5793(01)02915-5keywords:
subject
Has Abstractpub_date
2001-10-19 00:00:00pages
59-66issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02915-5journal_volume
507pub_type
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