L-tyrosine is the precursor of PQQ biosynthesis in Hyphomicrobium X.


:A method was developed to study amino acids as possible precursors of PQQ biosynthesis. Cultures of Hyphomicrobium X, growing on [13C]methanol, were supplemented with unlabelled amino acids. Uptake and participation in metabolism were determined via gas chromatography/mass spectrometry of derivatized amino acids, obtained from hydrolyzed cellular protein, by measuring their 12C content. Several amino acids appeared to be incorporated into the protein to a significant extent, without degradation or conversion. Among these were the aromatic amino acids, L-tyrosine and L-phenylalanine. Using the same replacement approach, their incorporation into PQQ was determined by 1H- and 13C-NMR spectroscopy of purified PQQ obtained from the culture medium. It appeared that the complete carbon skeleton of tyrosine was present, forming the o-quinone and pyrrole-2-carboxylic acid moieties in PQQ, while phenylalanine was not incorporated at all. Starting with L-tyrosine, possible biosynthetic routes to PQQ are discussed.




FEBS letters


van Kleef MA,Duine JA




Has Abstract


1988-09-12 00:00:00














  • Activation of the WT1 tumor suppressor gene promoter by Pea3.

    abstract::Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Discenza MT,Vaz D,Hassell JA,Pelletier J

    更新日期:2004-02-27 00:00:00

  • Enhancement of phospholipase A2 activation by phosphatidic acid endogenously formed through phospholipase D action in rat peritoneal mast cell.

    abstract::Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA2 activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p-bromophenacyl bromide. Upon exposure of the cells to ethanol in a h...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato T,Ishimoto T,Akiba S,Fujii T

    更新日期:1993-05-24 00:00:00

  • Sequence specificity in CpG mutation hotspots.

    abstract::CpG dinucleotides are efficiently methylated in vertebrate genomes except in the CpG islands having a high C+G content. Methylated CpGs are the single most mutated dinucleotide. Sequences surrounding disease causing CpG mutation sites were analyzed from locus-specific mutation databases. Both tetra- and heptanucleotid...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ollila J,Lappalainen I,Vihinen M

    更新日期:1996-11-04 00:00:00

  • Sizing of single-stranded regions in double-stranded DNA by preparative benzoylated DEAE-cellulose chromatography.

    abstract::The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybri...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Norris MD,Stewart BW

    更新日期:1988-02-15 00:00:00

  • Pore mutations affecting tetrameric assembly and functioning of the potassium channel KcsA from Streptomyces lividans.

    abstract::Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Splitt H,Meuser D,Borovok I,Betzler M,Schrempf H

    更新日期:2000-04-21 00:00:00

  • Evidence for the presence of a component of the Mn complex of the photosystem II reaction centre which is exposed to water in the S(2) state of the water oxidation complex.

    abstract::The interaction of water oxidising photosystem II preparations with the aqueous environment has been investigated using electron spin echo envelope modulation spectroscopy in the presence of 2H(2)O. The spectra show interaction of 2H of 2H(2)O with the preparation in the S(2) state. The component interacting with wate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Evans MC,Rich AM,Nugent JH

    更新日期:2000-07-14 00:00:00

  • In vitro production of cytokines is influenced by sulfatide and its precursor galactosylceramide.

    abstract::Effects of sulfatide and its precursor galactosylceramide (gal-cer) on the kinetics of production of cytokines were studied. In human mononuclear leucocytes, gal-cer but not sulfatide induced significantly increased amounts of interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF) mRNA. In phytohemagglutinin-sti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bovin LF,Fredman P,Månsson JE,Buschard K,Bendtzen K

    更新日期:1999-07-23 00:00:00

  • Dual role of the σ factor in primer RNA synthesis by bacterial RNA polymerase.

    abstract::Bacterial RNA polymerase (RNAP) serves as a primase during replication of single-stranded plasmids and filamentous phages. Primer RNA (prRNA) synthesis from the origin regions of these replicons depends on the σ factor that normally participates in promoter recognition. However, it was proposed that σ may not be requi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Esyunina D,Pupov D,Kulbachinskiy A

    更新日期:2019-02-01 00:00:00

  • A TY1 element is inserted in the CYR1 control region of Saccharomyces cerevisiae strain AB320.

    abstract::Southern blotting using a 5'-proximal probe of the Saccharomyces cerevisiae CYR1 gene has revealed heterogeneity in laboratory strains. It is demonstrated that strain AB320 contains a Ty1 element inserted in the promoter region of CYR1. The Ty1 orientation suggests that transcription of CYR1 is initiated downstream fr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lenzen G,Masson P,Jacquemin JM,Danchin A

    更新日期:1987-07-13 00:00:00

  • 2-Arachidonoylglycerol, an endogenous cannabinoid receptor agonist: identification as one of the major species of monoacylglycerols in various rat tissues, and evidence for its generation through CA2+-dependent and -independent mechanisms.

    abstract::The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kondo S,Kondo H,Nakane S,Kodaka T,Tokumura A,Waku K,Sugiura T

    更新日期:1998-06-12 00:00:00

  • Biochemical evidence for histidine oxidation in photosystem II depleted of the Mn-cluster for O2-evolution.

    abstract::Flash excitation of Tris-treated photosystem II (PSII) membranes generated a thermoluminescence band peaking at -20 degrees C, indicating that a positively charged oxidizing equivalent is stably accumulated on donor side of PSII even in the absence of the Mn-cluster. This oxidizing equivalent was sensitive to low conc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ono T,Inoue Y

    更新日期:1991-01-28 00:00:00

  • Evidence of a tissue-restricting DNA regulatory element in the mouse IRBP promoter.

    abstract::The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boatright JH,Knox BE,Jones KM,Stodulkova E,Nguyen HT,Padove SA,Borst DE,Nickerson JM

    更新日期:2001-08-24 00:00:00

  • The narcotic antagonist naltrexone has a biphasic effect on the nicotinic acetylcholine receptor.

    abstract::It is known that narcotic antagonists interact with many cholinergic pathways but less in known about specific mechanisms. Using neonatal rat myoballs to study single channel behaviour of the acetylcholinegated nicotinic receptor, it was found that micromolar concentrations of naltrexone had no effect on channel condu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Madsen BW,Albuquerque EX

    更新日期:1985-03-11 00:00:00

  • Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1.

    abstract::Botulinum neurotoxins (BoNTs), the causative agent of human botulism, are the most potent naturally occurring toxins known. BoNT/A1, the most studied BoNT, is also used as an important biopharmaceutical. In this study, the biological activity of BoNT/A1 is compared to that of BoNT/A2 using neuronal cell models. The da...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pier CL,Chen C,Tepp WH,Lin G,Janda KD,Barbieri JT,Pellett S,Johnson EA

    更新日期:2011-01-03 00:00:00

  • Evidence for a hydroxy-aluminium polymer (Al13) in synaptosomes.

    abstract::The presence of the hydroxy-aluminium polymer (Al13-(OH)24O4(H2O)12(7+)) was noticed inside synaptosomes when synaptosomes were incubated with Al(NO3)3 at neutral pH values. Dysprosium nitrate (Dy(NO3)3)--a shift reagent--facilities the identification of the Al13 species distinctly inside the synaptosomes. 27Al NMR wa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rao GV,Rao KS

    更新日期:1992-10-12 00:00:00

  • Insertion and translocation of proteins into and through membranes.

    abstract::In prokaryotic and eukaryotic organisms, proteins are efficiently sorted to reach their final destinations in a whole range of subcellular compartments. Targeting is mediated by hydrophobic signal sequences or hydrophilic targeting sequences depending upon the compartment, these sequences being often processed. Protei...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Lazdunski CJ,Benedetti H

    更新日期:1990-08-01 00:00:00

  • An improved purification of lactose permease.

    abstract::The integral membrane protein lactose permease of Escherichia coli was purified to homogeneity in detergent micelles. No other proteins could be detected in the final product. The molar ratio of lactose permease to lipid was less than 0.2 in detergent solution, thus the preparation was essentially lipid-free. All mole...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dornmair K

    更新日期:1988-08-01 00:00:00

  • Functional disruption of IEX-1 expression by concatemeric hammerhead ribozymes alters growth properties of 293 cells.

    abstract::The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Grobe O,Arlt A,Ungefroren H,Krupp G,Fölsch UR,Schmidt WE,Schäfer H

    更新日期:2001-04-13 00:00:00

  • Polysulfide exerts a protective effect against cytotoxicity caused by t-buthylhydroperoxide through Nrf2 signaling in neuroblastoma cells.

    abstract::Polysulfide is a bound sulfur species derived from endogenous H2S. When mouse neuroblastoma, Neuro2A cells were exposed to tert-butyl hydroperoxide after treatment with polysulfide, a significant decline in cell toxicity was observed. Rapid uptake of polysulfides induced translocation of Nrf2 into the nucleus, resulti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koike S,Ogasawara Y,Shibuya N,Kimura H,Ishii K

    更新日期:2013-11-01 00:00:00

  • The molecular signature of oncofusion proteins in acute myeloid leukemia.

    abstract::Acute myeloid leukemia (AML) associated translocations often cause gene fusions that encode oncofusion proteins. Although many of the breakpoints involved in chromosomal translocations have been cloned, in most cases the role of the chimeric proteins in tumorigenesis is not elucidated. Here we will discuss the fusion ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Martens JH,Stunnenberg HG

    更新日期:2010-06-18 00:00:00

  • Ceramide-1-phosphate: a novel regulator of cell activation.

    abstract::Ceramide-1-phosphate (CER-1-P) is emerging as a novel bioactive sphingolipid. It is formed by phosphorylation of ceramide catalyzed by ceramide kinase, and has been implicated in different cellular processes. Cer-1-P is mitogenic for fibroblasts, blocks apoptosis in macrophages, controls phagocytosis in neutrophils, a...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Gómez-Muñoz A

    更新日期:2004-03-26 00:00:00

  • Molecular mechanism and physiological role of pexophagy.

    abstract::Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Manjithaya R,Nazarko TY,Farré JC,Subramani S

    更新日期:2010-04-02 00:00:00

  • A naturally occurring bacterial Tat signal peptide lacking one of the 'invariant' arginine residues of the consensus targeting motif.

    abstract::Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hinsley AP,Stanley NR,Palmer T,Berks BC

    更新日期:2001-05-18 00:00:00

  • Processing of secretogranin II by prohormone convertases: importance of PC1 in generation of secretoneurin.

    abstract::Secretoneurin is a recently characterized neuropeptide present in the primary amino acid sequence of secretogranin II. We investigated the proteolytic processing of secretogranin II by prohormone convertases in vivo in a cellular system using the vaccinia virus system. Both PC1 and PC2 can cleave the secretogranin II ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hoflehner J,Eder U,Laslop A,Seidah NG,Fischer-Colbrie R,Winkler H

    更新日期:1995-03-06 00:00:00

  • Cytochrome d induction in Escherichia coli growing under unfavorable conditions.

    abstract::Growth of E. coli in the presence of the protonophorous uncoupler pentachlorophenol is shown to strongly enhance levels of cytochrome d, a putative Na(+)-motive oxidase. This effect was found to be arrested by chloramphenicol and stimulated by high Na+ concentration in the growth medium. The induction of cytochrome d ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bogachev AV,Murtazina RA,Skulachev VP

    更新日期:1993-12-20 00:00:00

  • Nuclear protein transport is functionally conserved between yeast and higher eukaryotes.

    abstract::The ability of a yeast nuclear protein to be transported into the nucleus of a higher eukaryotic cell was investigated. Mcm1, a transcriptional activator protein from Saccharomyces cerevisiae, was microinjected into the cytoplasm of Xenopus laevis frog oocytes. Mcm1 was imported into the oocyte nucleus indicating that...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wagner P,Hall MN

    更新日期:1993-04-26 00:00:00

  • Variable RNA polymerase populations in the life cycle of Trypanosoma brucei.

    abstract::DNA-dependent RNA polymerase from blood forms and culture forms of the parasitic protozoan Trypanosoma brucei was resolved into multiple peaks of activity by DEAE-Sephadex chromatography. The enzyme from the two forms was found to be different by several criteria, suggesting that it is subject to developmental control...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tittawella I

    更新日期:1988-01-25 00:00:00

  • A GFP fluorescence-based approach to determine detergent insolubility of the human serotonin1A receptor.

    abstract::Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kalipatnapu S,Chattopadhyay A

    更新日期:2004-10-22 00:00:00

  • Retinol binding protein expression is induced in HepG2 cells by zinc deficiency.

    abstract::Zinc (Zn) deficiency is often associated with low plasma vitamin A (retinol) concentrations. It has been suggested that the reduction in plasma retinol is secondary to reduced liver retinol binding protein (RBP) synthesis. In the present study, RBP expression was determined in HepG2 cells cultured in either Zn adequat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Satre MA,Jessen KA,Clegg MS,Keen CL

    更新日期:2001-03-02 00:00:00

  • A complex peptide-sorting signal, but no mRNA signal, is required for the Sec-independent transport of Ist2 from the yeast ER to the plasma membrane.

    abstract::The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Franz A,Maass K,Seedorf M

    更新日期:2007-02-06 00:00:00