Abstract:
:Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid into prostanoids which are involved in apoptosis and inflammation. Two distinct COXs have been identified: COX-1 which is constitutively expressed and COX-2 which is induced by different products such as tumor promoters or growth factors. Previously, we demonstrated that a plant steroid, diosgenin, was a new megakaryocytic differentiation inducer of human erythroleukemia cells. In our study, we investigated the effect of diosgenin on the proliferation rate, cell cycle distribution and apoptosis in the human osteosarcoma 1547 cell line. The effects of this compound were also tested on COX expression and COX activities. Diosgenin treatment caused an inhibition of 1547 cell growth with a cycle arrest in G1 phase and apoptosis induction. Moreover, we found a correlation between p53, p21 mRNA expression and nuclear factor-kappaB activation and we observed a time-dependent increase in PGE2 synthesis after diosgenin treatment.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Moalic S,Liagre B,Corbière C,Bianchi A,Dauça M,Bordji K,Beneytout JLdoi
10.1016/s0014-5793(01)02924-6keywords:
subject
Has Abstractpub_date
2001-10-12 00:00:00pages
225-30issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02924-6journal_volume
506pub_type
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