Abstract:
:Feeding insects introduce oral secretions (OS) into the wounded tissue of the attacked plant. Various OS-derived molecules must be involved in subsequent processes including the induction of plant defence reactions. Using the planar lipid bilayer membrane technique, isolated OS were analyzed with respect to their membrane activities. Transmembrane ion fluxes were generated by OS of eight different lepidopteran larvae, all of which form comparable ion channels in artificial membranes. Currents were characterized by long lasting open times and conductivities from 250pS up to 1100pS. Channels formed by Spodoptera exigua secretions showed a preference for cations over anions. OS also induced a transient increase of the cytosolic calcium concentration in soybean cells, determined by the aequorin technique. Known compounds of the OS, fatty-acid-glutamine conjugates, also interfered with the membrane but were unable to form stable channels. Since ion fluxes and depolarization are early responses upon insect feeding, OS-derived components may be involved in the elicitation process by direct interaction with the plant membranes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Maischak H,Grigoriev PA,Vogel H,Boland W,Mithöfer Adoi
10.1016/j.febslet.2007.01.067subject
Has Abstractpub_date
2007-03-06 00:00:00pages
898-904issue
5eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00114-7journal_volume
581pub_type
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