Abstract:
:Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylornithine, the DNA binding capacity of the transcription factor HSF induced by heat shock undergoes a severe and prompt deactivation. Replenishment of the spermidine pool before heat shock re-establishes the DNA binding activity of HSF and the inducibility of hsp 70 mRNA. Similar to HSF, but with a different time-course, the DNA binding of the transcription factor AP-1 activated by heat shock is also impaired in spermidine-depleted cells and reversed by exogenous spermidine. STAT3 provides an example of a transcription factor slightly activated by heat shock but insensitive to polyamine decrease.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Desiderio MA,Dansi P,Tacchini L,Bernelli-Zazzera Adoi
10.1016/s0014-5793(99)00873-xkeywords:
subject
Has Abstractpub_date
1999-07-16 00:00:00pages
149-53issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00873-Xjournal_volume
455pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80394-0
更新日期:1987-10-05 00:00:00
abstract::MicroRNAs mainly inhibit coding genes and long non-coding RNA expression. Here, we report that hsa-miR-125b and oncogene SIRT7/oncogenic long non-coding RNA MALAT1 were inversely expressed in bladder cancer. Hsa-miR-125b mimic down-regulated, whereas hsa-miR-125b inhibitor up-regulated the expression of SIRT7 and MALA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:
更新日期:2013-11-29 00:00:00
abstract::In Alzheimer's disease, beta-amyloid peptides (betaA(1-40) and betaA(1-42)) are deposited on the brain cell surfaces as neurotoxic plaques. Some reports indicate that small heat shock proteins, Hsp27 and alphaB-crystallin, colocalize in the plaques, but their functions are not known. Interaction between betaA and alph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02136-0
更新日期:2000-11-03 00:00:00
abstract::Cytochrome c oxidase has been isolated from beef heart mitochondria and labeled with the fluorochrome eosin-5-maleimide (EMA) after pretreatment with mersalyl. On SDS-polyacrylamide gels, EMA fluorescence and absorption occurred at a single band corresponding to subunit III. Since only Cys 115 of the two cysteinyl res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80664-5
更新日期:1985-05-06 00:00:00
abstract::Heme is degraded by heme oxygenase to form iron, carbon monoxide (CO), and biliverdin. However, information about the catabolism of heme in erythroid cells is limited. In this study, we showed the production and export of bilirubin in murine erythroleukemia (MEL) cells. The production of bilirubin by MEL cells was enh...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12178
更新日期:2016-05-01 00:00:00
abstract::Amino acid residues 3-15 of mycobacterial HSP60 define a dominant T-cell epitope for HLA-DR3+ve humans and Mamu-DR3+ve rhesus monkeys. Our results show that Mamu-DR3 molecules on PBMC can be efficiently loaded in vivo with the above-mentioned peptides when they are intravenously injected encapsulated in liposomes, but...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00493-6
更新日期:1997-06-02 00:00:00
abstract::We identified a CpG island at the 5' end of murine leukemia inhibitory factor gene (LIF). The CpG island is 0.6 kb long and covers most of the first exon and first intron. The region is non-methylated, its G+C content is 65% and relative frequency of CpG dinucleotide is 0.7. The block of 150 nucleotides, which is 72% ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80058-3
更新日期:1993-03-15 00:00:00
abstract::Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.10.028
更新日期:2014-12-20 00:00:00
abstract::A pronounced transitory increase of histone acetyltransferase (EC 2.3.1.48) activity before the onset of DNA replication is shown to occur in regenerating rat liver. This effect is followed by an increase in the H1/H1(0) ratio at the start of DNA synthesis; H1(0) de is replaced by H1. Histone acetylation and H1/H1(0) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80257-6
更新日期:1988-09-26 00:00:00
abstract::Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01777-9
更新日期:2000-01-21 00:00:00
abstract::Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subunit with a molecular...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(87)80654-3
更新日期:1987-06-15 00:00:00
abstract::Three mutant polypeptides of the type I 37-residue winter flounder 'antifreeze' protein have been synthesized. All four threonine residues in the native peptide were been mutated to serine, valine and glycine respectively and two additional salt bridges were incorporated into the sequences in order to improve aqueous ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00652-8
更新日期:1998-07-03 00:00:00
abstract::Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.08.062
更新日期:2004-10-08 00:00:00
abstract::The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80522-1
更新日期:1970-12-01 00:00:00
abstract::Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80706-m
更新日期:1992-08-03 00:00:00
abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00719-4
更新日期:1998-07-10 00:00:00
abstract::In endocrine cells the regulated secretion of hormones, peptides, enzymes and neurotransmitters into the external medium occurs when mature secretory granules fuse with the plasma membrane. Secretory granules form at the trans-Golgi network (TGN) by envelopment of the dense-core aggregate of regulated secretory protei...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(91)80805-d
更新日期:1991-07-22 00:00:00
abstract::Disruption of the gene pgil of Saccharomyces cerevisiae, which codes for phosphoglucose isomerase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6-phosphate was much higher in mutant than in wild-type cells. Phosphorylase a activity and the s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81325-g
更新日期:1992-09-28 00:00:00
abstract::The yeast PMR2/ENA1 gene encodes an ATPase involved in sodium extrusion and induced by NaCl. At low salt concentrations (0.3 M) induction is mediated by the HOG-MAP kinase pathway, a system activated by non-specific osmotic stress. At high salt concentrations (0.8 M) induction is mediated by the protein phosphatase ca...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00157-3
更新日期:1996-03-11 00:00:00
abstract::Acetaldehyde (1-20 mM) was metabolized at high rates and in a dose-dependent manner in isolated human and baboon kidney-cortex tubules. Acetaldehyde removal was accompanied by a large accumulation of acetate in both human and baboon tubules. By contrast, a large synthesis of ethanol was observed only in baboon tubules...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80767-6
更新日期:1987-05-25 00:00:00
abstract::We previously reported that IκBL prevents experimental autoimmune arthritis. The molecular mechanism, however, still remains unclear. In contrast to four splicing-isoforms of IκBL in human, two isoforms were identified in mouse. The major isoform IκBL-α(S) suppressed LPS-induced NF-κB activation and transcription of T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.10.024
更新日期:2011-11-16 00:00:00
abstract::A new approach is suggested for delineating the structural and functional amino acid residues in proteins with known three-dimensional structure, basing on the involvement of residues in intramolecular hydrophobic and hydrophilic interactions and additional information about the conservativity of the residues. The app...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01212-w
更新日期:1995-11-13 00:00:00
abstract::LipL and Cpr19 are nonheme, mononuclear Fe(II)-dependent, α-ketoglutarate (αKG):UMP oxygenases that catalyze the formation of CO2 , succinate, phosphate, and uridine-5'-aldehyde, the last of which is a biosynthetic precursor for several nucleoside antibiotics that inhibit bacterial translocase I (MraY). To better unde...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12554
更新日期:2017-02-01 00:00:00
abstract::Cytochrome bd is a bacterial respiratory oxidase carrying three hemes but no copper. We show that nitric oxide (NO) reacts with the intermediate F of cytochrome bd from Azotobacter vinelandii: (i) with a 1:1 stoichiometry, (ii) rapidly (k=1.2 +/- 0.1 x 10(5)M(-1)s(-1) at 20 degrees C), and (iii) yielding the oxidized ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.07.072
更新日期:2006-09-04 00:00:00
abstract::The biosynthesis of human tumor necrosis factor (hTNF) was studied. The amino-terminal extension of the hTNF precursor (26 kDa polypeptide) was not cleaved off in a cell-free system supplemented with dog pancreas microsomes. Correct maturation of pre-hTNF was nevertheless not restricted to the macrophage system: in th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80306-4
更新日期:1986-03-03 00:00:00
abstract::By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00975-2
更新日期:1998-08-28 00:00:00
abstract::Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81331-s
更新日期:1993-02-22 00:00:00
abstract::Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80835-1
更新日期:1982-11-08 00:00:00
abstract::HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00935-7
更新日期:1999-07-30 00:00:00
abstract::A-disintegrin and metalloproteinase 10 (ADAM10) is involved in the generation of amyloid-β (Aβ) during amyloid precursor protein (APP) processing, and has a protective effect against Aβ neurotoxicity. We explored how metallothionein-III (MT-III) is regulated in the non-amyloidogenic pathway to generate soluble APPα (s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.05.017
更新日期:2014-06-27 00:00:00