The directionality of processive enzymes acting on recalcitrant polysaccharides is reflected in the kinetic signatures of oligomer degradation.

Abstract:

:The enzymatic degradation of the closely related insoluble polysaccharides; cellulose (β(1-4)-linked glucose) by cellulases and chitin (β(1-4)-linked N-acetylglucosamine) by chitinases, is of large biological and economical importance. Processive enzymes with different inherent directionalities, i.e. attacking the polysaccharide chains from opposite ends, are crucial for the efficiency of this degradation process. While processive cellulases with complementary functions differ in structure and catalytic mechanism, processive chitinases belong to one single protein family with similar active site architectures. Using the unique model system of Serratia marcescens with two processive chitinases attacking opposite ends of the substrate, we here show that different directionalities of processivity are correlated to distinct differences in the kinetic signatures for hydrolysis of oligomeric tetra-N-acetyl chitotetraose.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Hamre AG,Schaupp D,Eijsink VG,Sørlie M

doi

10.1016/j.febslet.2015.05.034

subject

Has Abstract

pub_date

2015-07-08 00:00:00

pages

1807-12

issue

15

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(15)00428-7

journal_volume

589

pub_type

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