Abstract:
:Extended X-ray absorption fine structure (EXAFS) spectra at the Cu K-edge have been recorded of the oxidized and reduced form at pH 3.5 of rusticyanin, the type-1 or 'blue'-copper protein from Thiobacillus ferrooxidans. The EXAFS of oxidized rusticyanin is well simulated with models assuming a ligand set of 2 N(His) and 1 S(Cys) at 1.99 and 2.16 A, respectively. Upon reduction, the average Cu-N ligand distance increases by approx. 0.08A. For both redox states studied, the fit by the simulation is significantly improved by including a contribution of an additional sulfur ligand at approx. 2.8 A. From comparison with structural data of other blue-copper proteins, it is concluded that the copper coordination environment is relatively rigid, which may be a clue to its high redox potential.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Holt SD,Piggott B,Ingledew WJ,Feiters MC,Diakun GPdoi
10.1016/0014-5793(90)81133-9subject
Has Abstractpub_date
1990-08-20 00:00:00pages
117-21issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(90)81133-9journal_volume
269pub_type
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