Abstract:
:Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid and glucose homeostasis. Here we show that FXR is expressed and functional, as assessed by target gene expression analysis, in human islets and beta-cell lines. FXR is predominantly cytosolic-localized in the islets of lean mice, but nuclear in obese mice. Compared to FXR+/+ mice, FXR-/- mice display a normal architecture and beta-cell mass but the expression of certain islet-specific genes is altered. Moreover, glucose-stimulated insulin secretion (GSIS) is impaired in the islets of FXR-/- mice. Finally, FXR activation protects human islets from lipotoxicity and ameliorates their secretory index.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Popescu IR,Helleboid-Chapman A,Lucas A,Vandewalle B,Dumont J,Bouchaert E,Derudas B,Kerr-Conte J,Caron S,Pattou F,Staels Bdoi
10.1016/j.febslet.2010.04.068subject
Has Abstractpub_date
2010-07-02 00:00:00pages
2845-51issue
13eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00372-8journal_volume
584pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80845-l
更新日期:1993-12-28 00:00:00
abstract::Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80305-m
更新日期:1991-03-25 00:00:00
abstract::Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80704-3
更新日期:1985-07-08 00:00:00
abstract::Prodigiosin 25-C inhibited the accumulation of 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange in the acidic compartments of baby hamster kidney cells with little perturbation of cellular ATP levels. In rat liver lysosomes, prodigiosin 25-C inhibited the proton pump activity with an IC50 of a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01446-8
更新日期:1995-02-06 00:00:00
abstract::Analysis of glutamic acid decarboxylase (GDC) (EC 4.1.1.15) from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80263-7
更新日期:1989-03-27 00:00:00
abstract::In the present study we have examined the levels and phosphorylation state of the insulin receptor and insulin receptor substrate 1 (IRS-1) as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of rats treated with glucagon. There was a decrease in the insulin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00809-n
更新日期:1995-08-14 00:00:00
abstract::Many receptors coupled to inhibitory Go/Gi-type G proteins often also produce stimulatory signals like Ca2+ mobilisation. When expressed in CHO cells the alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpkha2C mobilised Ca2+. These responses were strongly reduced by the P2Y-purinoceptor antagonist suramin. A large ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00664-4
更新日期:1998-07-03 00:00:00
abstract::Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.13031
更新日期:2018-04-01 00:00:00
abstract::The interaction of bovine pancreatic trypsin inhibitor (BPTI) and bovine tryptase, which are co-localized in the same granules of bovine mast cells, has been analyzed at 30 degrees C in 0.1 M Tris-HCl, pH 8.0. The analysis has unravelled that the functional unit of bovine tryptase is formed of (at least) four binding ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00286-i
更新日期:1995-04-17 00:00:00
abstract::The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01350-a
更新日期:1995-01-03 00:00:00
abstract::A role for the cytokines produced by tissue-infiltrated inflammatory cells (mainly T-lymphocytes and mast cells) in the pathophysiology of fibrosis has been suggested by several groups. Among the products of these cells, interleukin-4 (IL-4) might be one of the factors involved in the initiation of the fibrotic proces...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80448-p
更新日期:1992-05-18 00:00:00
abstract::Sufficient highly purified native pea cytosolic ascorbate peroxidase was obtained to characterize some of its kinetic and spectral properties. Its rate constant for compound I formation from reaction with H2O2 is 4.O x 10(7) M-1 s-1, somewhat faster than is typical for peroxidases. Compound I has the typical optical s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00562-5
更新日期:1996-07-01 00:00:00
abstract::The high affinity (low Km) cyclic GMP phosphodiesterase (PDE) is activated by GTP, while the cyclic AMP PDE is not. GTP and its hydrolysis-resistant analogue, guanylylimidodiphosphate (GppNHp), display a half-maximal stimulating effect at almost the same concentration (5 X 10(-6) M). The GTP stimulating effect is not ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80574-2
更新日期:1983-07-04 00:00:00
abstract::Ferric reductase B (FerB) is a flavin mononucleotide (FMN)-containing NAD(P)H:acceptor oxidoreductase structurally close to the Gluconacetobacter hansenii chromate reductase (ChrR). The crystal structure of ChrR was previously determined with a chloride bound proximal to FMN in the vicinity of Arg101, and the authors ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13359
更新日期:2019-04-01 00:00:00
abstract::Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13588
更新日期:2020-01-01 00:00:00
abstract::We investigated insulin's effect on intestinal lipid, transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17-20 weeks) reduced triglyceride and chylomicron production and inhibit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00896-4
更新日期:1996-09-16 00:00:00
abstract::Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02498-x
更新日期:2001-06-01 00:00:00
abstract::The L1CAM antibody A10-A3 efficiently reduces tumor growth in a nude mouse model. Here, we describe the crystal structure of the Fab fragment of A10-A3 determined at 2.0 angstrom resolution. The A10-A3 antibody H3 loop reveals a characteristic arrangement of exposed aromatic residues that may play an important role in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.11.028
更新日期:2011-01-03 00:00:00
abstract::Neopterin is synthesized by human monocyte-derived macrophages primarily upon stimulation with the cytokine interferon-gamma. We studied the influence of neopterin on the generation of reactive oxygen species (ROS) in human peripheral blood neutrophils. Radical formation was measured using a biochemiluminometer. Neutr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00796-8
更新日期:2003-08-14 00:00:00
abstract::Gap junctions and their connexin components are indispensable in mediating the cellular coordination required for tissue and organ homeostasis. The critical nature of their existence mandates a connection to disease while at the same time offering therapeutic potential. Therapeutic intervention may be offered through ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.02.047
更新日期:2014-04-17 00:00:00
abstract::Ample evidence indicates that sphingosine-1-phosphate (SPP) can serve as an intracellular second messenger regulating calcium mobilization, and cell growth and survival. Moreover, the dynamic balance between levels of the sphingolipid metabolites, ceramide and SPP, and consequent regulation of opposing signaling pathw...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(00)01670-7
更新日期:2000-06-30 00:00:00
abstract::Tumor necrosis factor receptor associated factor 6 (TRAF6) is an important signaling molecule involved in a diverse array of physiological processes. It has been proposed that TRAF6, a RING finger-containing protein, acts as a ubiquitin ligase (E3) and a target for Lys-63 linked polyubiquitination mediated by Ubc13-Ue...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.038
更新日期:2004-05-21 00:00:00
abstract::Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81055-x
更新日期:1988-12-05 00:00:00
abstract::In the mouse macrophage-like cell line RAW 264, vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors, bafilomycin A(1) and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.08.055
更新日期:2007-10-02 00:00:00
abstract::A synthetic peptide, KKKKRFSFKKSFKLSGFSFKK, containing the phosphorylation sites of the acidic 80-87 kDa protein kinase C substrate was used to identify phosphopeptides in enzyme digests of this protein from mouse fibroblast C3H/10T1/2 cells. Stimulation of phosphorylation occurred, in vivo, with TPA at Ser7, Ser11 an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80557-w
更新日期:1992-02-10 00:00:00
abstract::The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80600-8
更新日期:1988-02-08 00:00:00
abstract::Spectrin repeats are three-helix bundle structures which occur in a large number of diverse proteins, either as single copies or in tandem arrangements of multiple repeats. They can serve structural purposes, by coordination of cytoskeletal interactions with high spatial precision, as well as a 'switchboard' for inter...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)03304-x
更新日期:2002-02-20 00:00:00
abstract::An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80931-r
更新日期:1991-07-29 00:00:00
abstract::To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. The 72 kDa protein was specifically enriched in the residues of cell clump/cell adh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00067-8
更新日期:1999-02-12 00:00:00
abstract::Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) techniq...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01338-5
更新日期:1996-12-16 00:00:00