Calcium mobilization by angiotensin II and neurotransmitter receptors expressed in Xenopus laevis oocytes.


:Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses up to 100-fold above basal levels. Ligand-induced light emission was also observed in oocytes injected with rat brain mRNA and stimulated with acetylcholine and glutamate. These findings demonstrate that mammalian AII receptors expressed in Xenopus oocytes are functionally linked to intracellular Ca2+ mobilization, and indicate the potential value of aequorin-injected oocytes for rapid and specific screening of mRNAs transcribed from expression libraries containing cloned receptor cDNAs.




FEBS letters


Sandberg K,Markwick AJ,Trinh DP,Catt KJ




Has Abstract


1988-12-05 00:00:00














  • Differential regulation of the STARD1 subfamily of START lipid trafficking proteins in human macrophages.

    abstract::The STARD1 subfamily of 'START' lipid trafficking proteins can reduce macrophage lipid content and inflammatory status (STARD1; StAR), and traffic cholesterol from endosomes (STARD3/MLN64). During macrophage differentiation, STARD1 mRNA and protein increase with sterol content, while the reverse is true for STARD3. St...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Borthwick F,Taylor JM,Bartholomew C,Graham A

    更新日期:2009-04-02 00:00:00

  • G protein-coupled receptor kinase 2 (GRK2): mechanisms of regulation and physiological functions.

    abstract::G protein-coupled receptor kinase 2 (GRK2) plays a key role in determining the rate and extent of G protein-coupled receptor (GPCR) desensitization and resensitization. Recent data indicate that GRK2 activity, subcellular distribution and expression are tightly regulated. The important physiological function of GRK2 a...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Aragay AM,Ruiz-Gómez A,Penela P,Sarnago S,Elorza A,Jiménez-Sainz MC,Mayor F Jr

    更新日期:1998-06-23 00:00:00

  • Identification of a novel Skp2-like mammalian protein containing F-box and leucine-rich repeats.

    abstract::The F-box protein Skp2 is important for S phase entry and binds to Skp1 and the cyclin A-Cdk2 complex. Here we report the cloning, analysis of genomic organization and characterization of a novel gene product related to Skp2 named FBL2. The human FBL2 gene was found to be a highly interrupted gene of at least 126.6 kb...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ilyin GP,Rialland M,Glaise D,Guguen-Guillouzo C

    更新日期:1999-10-01 00:00:00

  • Interaction between HERC1 and M2-type pyruvate kinase.

    abstract::HERC proteins are characterized by having one or more RCC1-like domains as well as a C-terminal HECT domain in their amino acid sequences. This has led researchers to suggest that they may act as both guanine nucleotide exchange factors and E3 ubiquitin ligases. Here we describe a physical interaction between the HECT...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Garcia-Gonzalo FR,Cruz C,Muñoz P,Mazurek S,Eigenbrodt E,Ventura F,Bartrons R,Rosa JL

    更新日期:2003-03-27 00:00:00

  • High affinity insulin binding by soluble insulin receptor extracellular domain fused to a leucine zipper.

    abstract::Insulin receptors (IRs) that are truncated at the end of the ectodomain form dimers that bind insulin with different characteristics to wild type receptors. These soluble IRs have lowered affinity for insulin compared with full-length IR, and exhibit linear Scatchard plots in contrast to the curvilinear plots obtained...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hoyne PA,Cosgrove LJ,McKern NM,Bentley JD,Ivancic N,Elleman TC,Ward CW

    更新日期:2000-08-11 00:00:00

  • Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of phosphorylase kinase.

    abstract::Synthetic oligonucleotides have been used to isolate a 1.85 kb clone containing the full length coding sequence for the catalytic subunit of rabbit skeletal muscle phosphorylase kinase from a cDNA library constructed in lambda gt10. Sequence analysis of the clone predicted an amino acid sequence in agreement with a pu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: da Cruz e Silva EF,Cohen PT

    更新日期:1987-08-10 00:00:00

  • Redox control of iron regulatory protein 2 stability.

    abstract::Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hausmann A,Lee J,Pantopoulos K

    更新日期:2011-02-18 00:00:00

  • The role of the C-terminal lysine in the hinge bending mechanism of yeast phosphoglycerate kinase.

    abstract::Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Adams B,Fowler R,Hudson M,Pain RH

    更新日期:1996-04-29 00:00:00

  • Probing DNA triple helix structure by chemical ligation.

    abstract::A series of oligomeric double and triple helical DNAs with irregular sequences of homopurine and homopyrimidine strands were prepared. DNA triplexes were identified by CD spectroscopy and thermal denaturation profiles (biphasic helix-coil transition). Condensation of oligonucleotides on single and double-stranded DNA ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dolinnaya NG,Pyatrauskene OV,Shabarova ZA

    更新日期:1991-06-24 00:00:00

  • Streptococci and the complement system: interplay during infection, inflammation and autoimmunity.

    abstract::Streptococci are a broad group of Gram-positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens hav...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Syed S,Viazmina L,Mager R,Meri S,Haapasalo K

    更新日期:2020-08-01 00:00:00

  • Rat glutathione transferase 8-8, an enzyme efficiently detoxifying 4-hydroxyalk-2-enals.

    abstract::Rat glutathione transferase 8-8 is one of the less abundant cytosolic glutathione transferases, accounting for approx. 1% of the total activity with 1-chloro-2,4-dinitrobenzene in liver. The enzyme is eluted at pH 6.3 upon chromatofocusing and has so far been identified in liver, kidney, lung and testis. Characteristi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jensson H,Guthenberg C,Alin P,Mannervik B

    更新日期:1986-07-28 00:00:00

  • Photosynthesis modulates the sign of phototaxis of wild-type Chlamydomonas reinhardtii. Effects of red background illumination and 3-(3',4'-dichlorophenyl)-1,1-dimethylurea.

    abstract::We have found that the sign of phototaxis, i.e. the direction of phototactic migration either towards or away from the light source, of wild-type Chlamydomonas reinhardtii depends on its photosynthetic activity. This accounts for the frequently reported transient positive phototaxis that precedes a negative phototaxis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi T,Watanabe M

    更新日期:1993-12-28 00:00:00

  • Bicarbonate requirement for the donor side of photosystem II.

    abstract::Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 microM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate ef...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Klimov VV,Allakhverdiev SI,Feyziev YaM,Baranov SV

    更新日期:1995-04-24 00:00:00

  • Enhanced toxicity of Bacillus thuringiensis Cry3A delta-endotoxin in coleopterans by mutagenesis in a receptor binding loop.

    abstract::We used site-directed mutagenesis to modify the Bacillus thuringiensis cry3A gene in amino acid residues 350-354. Two mutant toxins, A1 (R(345)A,Y(350)F,Y(351)F) and A2 (R(345)A,DeltaY(350), DeltaY(351)), showed significantly improved toxicity against Tenebrio molitor (yellow mealworm). The mutant toxin A1 was also mo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wu SJ,Koller CN,Miller DL,Bauer LS,Dean DH

    更新日期:2000-05-12 00:00:00

  • Heterogeneous turnover of terminal and core sugars within the carbohydrate chain of dipeptidylaminopeptidase IV isolated from rat liver plasma membrane.

    abstract::Dipeptidylaminopeptidase IV, a plasma membrane-bound glycoprotein, is characterized by an intramolecular heterogeneous turnover of the protein backbone and carbohydrate chain. The faster turnover of the latter is restricted only to the outer sugars. The inner core sugars D-mannose and N-acetyl-D-glucosamine turn over ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Volk BA,Kreisel W,Köttgen E,Gerok W,Reutter W

    更新日期:1983-10-31 00:00:00

  • On the presence of HMG proteins in yeast.

    abstract::Two polypeptides antigenically related to mammalian HMG1/2 have been detected in the yeast Saccharomyces cerevisiae. One exhibits an electrophoretic mobility in the range of mammalian HMG1/2 whereas the second polypeptide comigrates with yeast HMG S4. Evidence for the presence of HMG14/17 in yeast has not been obtaine...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Estruch F,Pérez-Ortín JE,José M,Puigdomènech P,Franco L

    更新日期:1986-03-03 00:00:00

  • ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells.

    abstract::The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

    更新日期:2004-07-16 00:00:00

  • Regulation of hyaluronate metabolism by progesterone in cultured fibroblasts from the human uterine cervix.

    abstract::The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tanaka K,Nakamura T,Takagaki K,Funahashi M,Saito Y,Endo M

    更新日期:1997-02-03 00:00:00

  • The microtubule associated protein syntabulin is required for glucose-stimulated and cAMP-potentiated insulin secretion.

    abstract::Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syn...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ying Y,Li L,Cao W,Yan D,Zeng Q,Kong X,Lu L,Yan M,Xu X,Qu J,Su Q,Ma X

    更新日期:2012-10-19 00:00:00

  • Purification of Escherichia coli 50 S ribosomal proteins by high performance liquid chromatography.

    abstract::The 50 S subunit proteins from the Escherichia coli ribosome were purified by size-exclusion, ion-exchange or reversed phase high performance liquid chromatography (HPLC) avoiding any precipitation or desalting procedures during isolation. Best resolution of this complex protein mixture was achieved by reversed phase ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kamp RM,Wittmann-Liebold B

    更新日期:1984-02-13 00:00:00

  • Structural insight into the interaction of ADP-ribose with the PARP WWE domains.

    abstract::The WWE domain is often identified in proteins associated with ubiquitination or poly-ADP-ribosylation. Structural information about WWE domains has been obtained for the ubiquitination-related proteins, such as Deltex and RNF146, but not yet for the poly-ADP-ribose polymerases (PARPs). Here we determined the solution...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: He F,Tsuda K,Takahashi M,Kuwasako K,Terada T,Shirouzu M,Watanabe S,Kigawa T,Kobayashi N,Güntert P,Yokoyama S,Muto Y

    更新日期:2012-11-02 00:00:00

  • GABAA receptor subunit beta1 is involved in the formation of protease-resistant prion protein in prion-infected neuroblastoma cells.

    abstract::Gamma-aminobutyric acid type A (GABAA) receptor beta1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease-resistant prion protein in prion-infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decrea...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kimura T,Ishikawa K,Sakasegawa Y,Teruya K,Sata T,Schätzl H,Doh-ura K

    更新日期:2010-03-19 00:00:00

  • Insulin processing and action in adipocytes: evidence for generation of insulin-containing vesicles by leupeptin and monensin.

    abstract::Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surfac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jeffrey IW,Flint DJ,Vernon RG,Mayer RJ

    更新日期:1985-03-25 00:00:00

  • Structure-activity relationships of aminoglycoside-arginine conjugates that bind HIV-1 RNAs as determined by fluorescence and NMR spectroscopy.

    abstract::We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dep...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lapidot A,Vijayabaskar V,Litovchick A,Yu J,James TL

    更新日期:2004-11-19 00:00:00

  • Choline but not its derivative betaine blocks slow vacuolar channels in the halophyte Chenopodium quinoa: implications for salinity stress responses.

    abstract::Activity of tonoplast slow vacuolar (SV, or TPC1) channels has to be under a tight control, to avoid undesirable leak of cations stored in the vacuole. This is particularly important for salt-grown plants, to ensure efficient vacuolar Na(+) sequestration. In this study we show that choline, a cationic precursor of gly...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pottosin I,Bonales-Alatorre E,Shabala S

    更新日期:2014-11-03 00:00:00

  • The human lactase-phlorizin hydrolase gene is located on chromosome 2.

    abstract::The lactase-phlorizin hydrolase gene was assigned to chromosome 2 by analysis of Southern blots of DNA from a panel of human-rodent cell hybrids containing characteristic sets of human chromosomes The hybridization probe used was a recently isolated cDNA clone of the human lactase-phlorizin hydrolase gene. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kruse TA,Bolund L,Grzeschik KH,Ropers HH,Sjöström H,Norén O,Mantei N,Semenza G

    更新日期:1988-11-21 00:00:00

  • Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast.

    abstract::Apoptosis specific proteins (ASP) are expressed in the cytoplasm of cultured mammalian cells of various lineages following induction of apoptosis. The cDNA encoding ASP has been cloned from a human expression library and has significant homology to the Saccharomyces cerevisiae APG5 gene which is essential for yeast au...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hammond EM,Brunet CL,Johnson GD,Parkhill J,Milner AE,Brady G,Gregory CD,Grand RJ

    更新日期:1998-04-03 00:00:00

  • Analogs of human epidermal growth factor which partially inhibit the growth factor-dependent protein-tyrosine kinase activity of the epidermal growth factor receptor.

    abstract::Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matsunami RK,Campion SR,Niyogi SK,Stevens A

    更新日期:1990-05-07 00:00:00

  • The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase.

    abstract::Macrophages contain arginase and an inducible NO synthase, demonstrated by using L-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite wi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hrabák A,Bajor T,Temesi A,Mészáros G

    更新日期:1996-07-22 00:00:00

  • Role of ChREBP in hepatic steatosis and insulin resistance.

    abstract::Non-alcoholic fatty liver disease is tightly associated with insulin resistance, type 2 diabetes and obesity, but the molecular links between hepatic fat accumulation and insulin resistance are not fully identified. Excessive accumulation of triglycerides (TG) is one the main characteristics of non-alcoholic fatty liv...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Denechaud PD,Dentin R,Girard J,Postic C

    更新日期:2008-01-09 00:00:00