Multisite phosphorylation of the 80 kDa (MARCKS) protein kinase C substrate in C3H/10T1/2 fibroblasts. Quantitative analysis of individual sites by solid-phase microsequencing.

abstract：:In addition to the selective amplification of cDNA from total RNA by the PCR method, the distinctive properties of ferredoxin-expressing colonies can be used for cloning a ferredoxin cDNA. This strategy for cloning and expressing cDNA in E. coli was applied to a sheep adreno-ferredoxin. The expressed sheep ferredoxin ...

journal_title：FEBS letters

authors： Matsuo Y,Tsujita M,Mizoguchi K,Ichikawa Y

更新日期：1992-04-20 00:00:00

abstract：:Organisms using the thiosulfate-oxidizing Sox enzyme system fall into two groups: group 1 forms sulfur globules as intermediates (Allochromatium vinosum), group 2 does not (Paracoccus pantotrophus). While several components of their Sox systems are quite similar, i.e. the proteins SoxXA, SoxYZ and SoxB, they differ by...

journal_title：FEBS letters

authors： Welte C,Hafner S,Krätzer C,Quentmeier A,Friedrich CG,Dahl C

更新日期：2009-04-17 00:00:00

abstract：:We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4+ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol poly...

journal_title：FEBS letters

authors： Sumner MT,Shears SB

更新日期：1997-08-11 00:00:00

abstract：:Incubation of cholinergic synaptic vesicles purified from the electric organ of Torpedo ocellata with radiolabeled [D-Ala2, D-Leu5]-enkephalin results in its accumulation in the vesicles. Kinetic analysis of the initial rates of uptake reveals temperature-dependent saturation kinetics which are best fitted by a single...

journal_title：FEBS letters

authors： Day NC,Wien D,Michaelson DM

更新日期：1985-04-08 00:00:00

abstract：:It was believed that there was no natural suppressor tRNA in Escherichia coli, however, it has been suggested that selC, relating to the synthesis of formate dehydrogenase of a selenoprotein [(1988) Nature 331, 723-725], codes for tRNA, even though the presence of tRNA has not yet been demonstrated. We detected the pr...

journal_title：FEBS letters

authors： Mizutani T,Maruyama N,Hitaka T,Sukenaga Y

更新日期：1989-04-24 00:00:00

abstract：:Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluoresc...

journal_title：FEBS letters

authors： Rephaeli A,Aviram A,Englender T,Shaklai M

更新日期：1988-08-29 00:00:00

abstract：:On April 1898 Camillo Golgi communicated to the Medical-Surgical Society of Pavia, the discovery of the "internal reticular apparatus", a novel intracellular organelle which he observed in nerve cells with the silver impregnation he had introduced for the staining of the nervous system. Soon after the discovery it bec...

journal_title：FEBS letters

authors： Mazzarello P,Garbarino C,Calligaro A

更新日期：2009-12-03 00:00:00

abstract：:Chronic exposure of a human myeloma cell line to dexamethasone resulted in a selection of cells resistant to the growth-inhibitory action of the glucocorticoid. Upon acute exposure of the parental myeloma cells to dexamethasone growth inhibition was associated with depression of ornithine decarboxylase (ODC, EC 4.1.1....

journal_title：FEBS letters

authors： Leinonen P,Alhonen-Hongisto L,Laine R,Jänne OA,Jänne J

更新日期：1987-05-04 00:00:00

abstract：:Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...

journal_title：FEBS letters

authors： Shin EY,Min DS,Shin JC,Shin KS,Hyun MS,Ha KS,Kim HS,Ahn HY,Kim EG

更新日期：2001-11-16 00:00:00

abstract：:The galactose transport system of Saccharomyces cerevisiae consists of one component which shows a Km value of approx. 4mM in growing cells. A rapid and irreversible inactivation of this transport is detected on impairment of protein synthesis. This inactivation shows the following characteristics: (i) it is due to ch...

journal_title：FEBS letters

authors： DeJuan C,Lagunas R

更新日期：1986-10-27 00:00:00

abstract：:The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The iden...

journal_title：FEBS letters

authors： Gibson LC,Hunter CN

更新日期：1994-09-26 00:00:00

abstract：:Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the major...

journal_title：FEBS letters

authors： Tanoue R,Kobayashi M,Katayama K,Nagata N,Wada H

更新日期：2014-05-02 00:00:00

abstract：:Adrenomedullin, a potently hypotensive peptide isolated from human pheochromocytoma, is known to elicit a rise in cAMP levels within mammalian endothelial and smooth muscle cells. Until now, however, little has been known about the adrenomedullin receptor. Recently, a group called receptor activity-modifying proteins ...

journal_title：FEBS letters

authors： Kamitani S,Asakawa M,Shimekake Y,Kuwasako K,Nakahara K,Sakata T

更新日期：1999-04-01 00:00:00

abstract：:Plasmids for the high-level overproduction of wild-type, and C- and N-terminal His-tagged MurG N-acetylglucosaminyl transferase from Escherichia coli were constructed. In complementation tests the three forms were active in vivo. After IPTG induction, growth, spheroplast formation and lysis, overproduced MurG proteins...

journal_title：FEBS letters

authors： Crouvoisier M,Mengin-Lecreulx D,van Heijenoort J

更新日期：1999-04-23 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:Fibroblasts were exposed to various concentrations of hydrogen peroxide and the removal of oxidized proteins was followed by determining protein-bound carbonyls. Fibroblasts are able to increase the turnover of metabolically radiolabeled proteins after treatment with hydrogen peroxide. It was demonstrated for the firs...

journal_title：FEBS letters

authors： Sitte N,Merker K,Grune T

更新日期：1998-12-04 00:00:00

abstract：:Subclinical inflammation is a candidate etiological factor in the pathogenesis of metabolic syndrome and in the progression of atherosclerosis. A central role for activated macrophages has been elucidated recently as important regulators of the inflammatory process in atherosclerosis. Macrophage differentiation and fu...

journal_title：FEBS letters

authors： Szanto A,Roszer T

更新日期：2008-01-09 00:00:00

abstract：:NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...

journal_title：FEBS letters

authors： Drincovich MF,Casati P,Andreo CS

更新日期：2001-02-09 00:00:00

abstract：:Connexins are transmembrane proteins that form gap junction- and hemi-channels. Once inserted into the membrane, hemi-channels (connexons) allow for diffusion of ions and small molecules (<1 kDa) between the extracellular space and the cytosol. Gap junction channels allow diffusion of similar molecules between the cyt...

journal_title：FEBS letters

authors： Meens MJ,Sabine A,Petrova TV,Kwak BR

更新日期：2014-04-17 00:00:00

abstract：:Sulfatide, which binds to influenza A viruses and prevents the viral infection, was found to inhibit the sialidase activities of influenza A viruses in a pH-dependent manner. The kinetic parameters of the effect of sulfatide on the sialidase activities of human influenza A viruses using fluorometric assay indicated th...

journal_title：FEBS letters

authors： Suzuki T,Takahashi T,Nishinaka D,Murakami M,Fujii S,Hidari KI,Miyamoto D,Li YT,Suzuki Y

更新日期：2003-10-23 00:00:00

abstract：:The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine...

journal_title：FEBS letters

authors： van Heyningen S

更新日期：1983-11-28 00:00:00

abstract：:All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme respons...

journal_title：FEBS letters

authors： Sahin U,Blobel CP

更新日期：2007-01-09 00:00:00

abstract：:ATP-dependent release of TCA-precipitable peptides from mitochondria-containing stroma (MCS) is described. The process is independent of ubiquitin, but is sensitive to hemin and to heat treatment. Neither chloramphenicol nor EGTA inhibit. 50% of the activity is dependent on charged tRNA. The peptides released from MCS...

journal_title：FEBS letters

authors： Dubiel W,Drung I,Müller M,Rapoport SM

更新日期：1989-07-03 00:00:00

abstract：:The fate of newly synthesized human immunodeficiency virus type 1 env gp160 was examined in COS-1 cells. The results of morphological chase experiments involving cycloheximide demonstrated that gp160 was retained in the Golgi apparatus for longer than the half-life of the molecule. The degradation of gp160 was insensi...

journal_title：FEBS letters

authors： Kimura T,Nishikawa M,Fujisawa J

更新日期：1996-07-15 00:00:00

abstract：:To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...

journal_title：FEBS letters

authors： Tomasselli AG,Frank R,Schiltz E

更新日期：1986-07-07 00:00:00

abstract：:Besides its canonical function of catalyzing the formation of telomeric repeats, many groups have recently reported non-canonical functions of hTERT in particular, and telomerase in general. Regulating transcription is the central basis of non-canonical functions of telomerase. However, unlike reverse transcriptase ac...

journal_title：FEBS letters

authors： Akıncılar SC,Low KC,Liu CY,Yan TD,Oji A,Ikawa M,Li S,Tergaonkar V

更新日期：2015-04-13 00:00:00

abstract：:ZAP-70 is a protein tyrosine kinase (PTK) that plays a critical role in T cell activation. To study the role of ZAP-70 catalytic activity in this process, a substrate capable of distinguishing between the activities of ZAP-70 and other PTKs would be useful, especially since it has recently been shown that ZAP-70 inter...

journal_title：FEBS letters

authors： Watts JD,Brabb T,Bures EJ,Wange RL,Samelson LE,Aebersold R

更新日期：1996-12-02 00:00:00

abstract：:The Wnt/beta-catenin signalling pathway is evolutionarily conserved across many species and plays important roles during embryogenesis. The Lee-Heinrich model (to recognize the contributions of R. Heinrich we will refer to the work proposed by Lee et al. [Lee, E., Salic, A., Krüger, R., Heinrich, R., Kirschner, M.W. (...

journal_title：FEBS letters

authors： Wawra C,Kühl M,Kestler HA

更新日期：2007-08-21 00:00:00

abstract：:Analysis of fluorimetric equilibrium-binding isotherms of a proteolytic fragment of bacteriophage T4 gene 32 protein (g32P) lacking residues 1-9 shows that this region contains the site responsible for the function of the NH2-terminal 'B' domain (residues 1-21). The end codon of the frameshift mutant g32P-PR201 has be...

journal_title：FEBS letters

authors： Casas-Finet JR

更新日期：1989-06-05 00:00:00

abstract：:Mutations in the presenilin (PS) genes PSI and PS2 are involved in Alzheimer's disease (AD). Recently, apoptosis-associated cleavage of PS proteins was identified. Here we demonstrate that PS1 as well as PS2 are substrates for different members of the caspase protein family. Remarkably, the caspases acting on PS1 coul...

journal_title：FEBS letters

authors： van de Craen M,de Jonghe C,van den Brande I,Declercq W,van Gassen G,van Criekinge W,Vanderhoeven I,Fiers W,van Broeckhoven C,Hendriks L,Vandenabeele P

更新日期：1999-02-19 00:00:00