Abstract:
:Besides its canonical function of catalyzing the formation of telomeric repeats, many groups have recently reported non-canonical functions of hTERT in particular, and telomerase in general. Regulating transcription is the central basis of non-canonical functions of telomerase. However, unlike reverse transcriptase activity of telomerase that requires only a few molecules of enzymatically active hTERT, non-canonical functions of hTERT or other telomerase components theoretically require several hundred copies. Here, we provide the first direct quantification of all the telomerase components in human cancer cell lines. We demonstrate that telomerase components do not exist in a 1:1 stoichiometric ratio, and there are several hundred copies of hTERT in cells. This provides the molecular basis of hTERT to function in other signaling cascades, including transcription.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Akıncılar SC,Low KC,Liu CY,Yan TD,Oji A,Ikawa M,Li S,Tergaonkar Vdoi
10.1016/j.febslet.2015.02.035subject
Has Abstractpub_date
2015-04-13 00:00:00pages
974-84issue
9eissn
0014-5793issn
1873-3468pii
S0014-5793(15)00127-1journal_volume
589pub_type
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