Abstract:
:Apoferritin has been selectively labeled with a maleimide nitroxide derivative at Cys-126, located in the hydrophilic 3-fold channels. Titration of this derivative with Fe(II), which gives rise to the initial Fe(III)-apoferritin complex, produces, at low metal-to-protein ratios, a decrease of the intensity of the label EPR signal due to the occurrence of a magnetic dipolar interaction. A label-metal distance ranging between 8-12 A can be estimated from titrations performed with VO(IV), which is known to bind in the 3-fold channels, and likewise produces a decrease in the label EPR signal. The present findings indicate that iron binds in the hydrophilic channels in its higher oxidation state and that these channels represent the metal entry route at least at low metal-to-protein ratios.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Desideri A,Stefanini S,Polizio F,Petruzzelli R,Chiancone Edoi
10.1016/0014-5793(91)80004-msubject
Has Abstractpub_date
1991-08-05 00:00:00pages
10-4issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80004-Mjournal_volume
287pub_type
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