Abstract:
:L-selectin guides lymphocytes into peripheral lymphoid tissues by recognizing glycoprotein ligands decorated with 6-sulfated sialyl Lewis x (sulfo sLex). Here we have used a rat peripheral lymph node high endothelial cell line (Ax) to study in detail the synthesis, expression and degradation of sLex epitope. We show here that Ax cells possess active alpha(1,3)fucosyltransferase Fuc-TVII, the enzyme responsible for the final fucosylation of sialyl-N-acetyllactosamine during sLex synthesis, and express sLex on the cell surface. Furthermore, these cells degrade sLex, primarily by desialylating it to neutral Lex epitopes by alpha(2,3)sialidase(s).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Majuri ML,Räbinä J,Niittymäki J,Tiisala S,Mattila P,Aavik E,Miyasaka M,Renkonen O,Renkonen Rdoi
10.1016/s0014-5793(99)00834-0keywords:
subject
Has Abstractpub_date
1999-07-16 00:00:00pages
97-100issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00834-0journal_volume
455pub_type
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