Abstract:
:The ANKRD1/CARP gene encodes a muscle-specific protein which has been implicated in transcriptional regulation and myofibrillar assembly. Several features at both the mRNA and protein levels define ANKRD1 as a gene whose expression is tightly regulated, and deregulated expression of this protein has been recently associated to human congenital heart disease. It is therefore crucial to define the intracellular pathways that regulate the ANKRD1 protein's steady-state levels. Here, we show that ANKRD1 is a short-lived protein whose levels are tightly regulated by the 26S proteasome. In addition, a critical role for a putative PEST motif was established, although other degrons within the ANKRD1 protein are likely implicated in the control of its intracellular levels.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Badi I,Cinquetti R,Frascoli M,Parolini C,Chiesa G,Taramelli R,Acquati Fdoi
10.1016/j.febslet.2009.07.001subject
Has Abstractpub_date
2009-08-06 00:00:00pages
2486-92issue
15eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00521-3journal_volume
583pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The possible participation of calmodulin in the activation of store-operated Ca2+ entry (SOC) in single rat hepatocytes was investigated microspectrofluorimetrically. SOC was triggered after discharging intracellular Ca2+ stores using the endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin in the absence of exter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00133-1
更新日期:1998-03-06 00:00:00
abstract::Red blood cells from patients homozygotes for hemoglobin S (HbS) have been studied using a computer-controlled microspectrophotometer, which allows measurements of spectra and dynamics to be undertaken in a single erythrocyte. Complete photodissociation of HbCO results in polymerization of intracellular deoxyhemoglobi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80299-0
更新日期:1988-08-15 00:00:00
abstract::We examined the mRNA levels of hypoxia-inducible factor-1alpha (HIF-1alpha) in bone marrow mesenchymal stem cells (bmMSCs) of eight osteoarthritis patients. BmMSC-1, expressing higher HIF-1alpha mRNA and protein than bmMSC-5, elicited higher matrix metalloproteinase-1 (MMP1) activity and stronger invasive capacity. In...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.033
更新日期:2008-07-23 00:00:00
abstract::A cDNA clone encoding the sequence of human pancreatic lipase (HPL) was subcloned into the baculovirus transfer vector pVL1392 and used in co-transfection of Spodoptera frugiperda (Sf9) insect cells with wild-type Autographa californica nuclear polyhedrosis virus (AcNPV) DNA. A single recombinant protein (50 kDa) secr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81044-z
更新日期:1993-07-19 00:00:00
abstract::We investigated in vitro effects of recombinant human thrombopoietin (TPO), or c-Mpl ligand, on human platelets. TPO induced rapid dose-dependent tyrosine phosphorylation of several proteins. We identified Janus tyrosine kinases, Tyk2 and JAK2, and a member of STAT (signal transducers and activators of transcription) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01072-m
更新日期:1995-10-23 00:00:00
abstract::The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.035
更新日期:2009-06-18 00:00:00
abstract::To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted ami...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00277-g
更新日期:1995-04-17 00:00:00
abstract::A functional rat heart Na(+)-Ca2+ exchanger gene has been obtained by splicing and ligating two partially overlapping clones isolated from a rat heart lambda ZAP cDNA library. The deduced primary structure of the protein encoded by the open reading frame corresponds to 971 amino acids, that can be organized into 12 tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81737-k
更新日期:1993-01-18 00:00:00
abstract::Epidermal growth factor (EGF) induces transformed phenotypes in EGF receptor-overexpressing NIH3T3 (ER12) cells. Tyrosine kinase inhibitors such as erbstatin and its stable analogue methyl 2,5-dihydroxycinnamate inhibited the EGF-induced phenotypic changes in these cells; while 5'-O-methylerbstatin, an inactive analog...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81491-4
更新日期:1992-12-21 00:00:00
abstract::The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01231-8
更新日期:1994-12-12 00:00:00
abstract::The modification of homeodomain-interacting protein kinase 2 (HIPK2) by small ubiquitin-like modifier 1 (SUMO-1) plays an important role in its targeting into the promyelocytic leukemia body, as well as in its differential interaction with binding partner, but the desumoylation of HIPK2 by SUMO-specific proteases is l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.10.010
更新日期:2005-11-07 00:00:00
abstract::Here we make a brief survey of the present state of antibiotic research and use. We also describe a novel antibiotic that contains a basic peptide covalently bound to a morpholino oligonucleotide. ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.10.048
更新日期:2014-01-03 00:00:00
abstract::The spatial relationship of polypeptides comprising the light-harvesting B800-850 complex of Chromatium minutissimum has been studied by means of chemical crosslinking of the isolated complex with cleavable, 1.2 nm-long dithiobis(succinimidyl propionate). The samples were analyzed by different types of electrophoresis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81650-o
更新日期:1993-03-29 00:00:00
abstract::Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80488-5
更新日期:1988-10-10 00:00:00
abstract::By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00975-2
更新日期:1998-08-28 00:00:00
abstract::Tissue factor pathway inhibitor (TFPI) inhibits the activity of coagulation factors VIIa and Xa through Kunitz domains, thereby inhibiting the activity of tissue factor. However, it has been shown that the C-terminal of this inhibitor is essential for the maximal anticoagulant activity of TFPI. We have investigated th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01663-4
更新日期:1999-12-17 00:00:00
abstract::We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00104-2
更新日期:2003-02-27 00:00:00
abstract::Vpu is an 81 amino acid protein encoded by HIV-1. Its role is to amplify viral release by two mechanisms: (i) docking to CD4 with the consequence of targeting CD4 for ubiquitine-mediated degradation, and (ii) formation of ion channels to enhance particle release. The intensive research on its in vivo function, combine...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(03)00782-8
更新日期:2003-09-18 00:00:00
abstract::Several lines of evidence led to the conclusion that mammalian ribosomal protein synthesis is a highly organized biological process in vivo. A wealth of data support the concept according to which tRNA aminoacylation, formation of the ternary complex on EF1A and delivery of aminoacyl-tRNA to the ribosome is a processi...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.027
更新日期:2010-01-21 00:00:00
abstract::Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.10.051
更新日期:2008-12-10 00:00:00
abstract::Cyclin B is a central regulator of transition from the G(2) phase of the cell cycle to mitosis. In mammalian cells two B-type cyclins have been characterised, cyclin B1 and B2. Both are expressed with a maximum in G(2) and their synthesis is mainly regulated on the transcriptional level. We show that a single cell cyc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00028-0
更新日期:2003-02-11 00:00:00
abstract::The relationship between the Fc epsilon receptor mediated stimulation of mast cells and the Ca2+ signal it induces were studied using thapsigargin (TG), a blocker of the endoplasmic reticulum Ca2+ pump. TG induced, in mucosal mast cells (RBL-2H3 line), a dose-dependent and an InsP3-independent increase in [Ca2+]i (fro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81311-9
更新日期:1992-09-28 00:00:00
abstract::The ability of (S)-alpha-methylproline (alpha-MePro) to stabilise reverse-turn conformations in the peptide hormone bradykinin (BK = Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) has been investigated. Two BK analogues containing alpha-MePro at position 3 or position 7 were synthesised and their conformations in aqueo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80541-n
更新日期:1992-02-10 00:00:00
abstract::Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.050
更新日期:2013-09-17 00:00:00
abstract::To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13124
更新日期:2018-07-01 00:00:00
abstract::Exposing a macrophage-like murine cell line to copper-oxidised low-density lipoprotein led to DNA fragmentation which was inhibited by the putative Ca2+/Mg2+ endonuclease inhibitor, zinc sulphate. DNA fragmentation preceded loss of membrane impermeability. These results suggest that apoptosis may be a mechanism of mac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80635-8
更新日期:1993-10-18 00:00:00
abstract::Single enzyme molecules display inevitable, stochastic fluctuations in their catalytic activity. In metabolism, for instance, the stochastic activity of individual enzymes is averaged out due to their high copy numbers per single cell. However, many processes inside cells rely on single enzyme activity, such as transc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.005
更新日期:2013-09-02 00:00:00
abstract::In this work, we have spectroscopically characterised CYP157C1 from Streptomyces coelicolor A3(2) which has the motif E(297)QSLW(301) rather than the invariant EXXR motif in the P450 K-helix. Site-directed mutagenesis of native E(297)QSLW(301) in CYP157C1 to E(297)ESLR(301) or E(297)QSRW(301) both containing standard ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.043
更新日期:2006-11-27 00:00:00
abstract::Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00424-2
更新日期:1994-05-23 00:00:00
abstract::We have identified and analyzed a 27-nucleotide sequence (U5 repressive element, designated as U5RE) at the U5 region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) which is required for HTLV-I basal transcriptional repression. The basal promoter strength of constructs that contained del...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01243-1
更新日期:1994-12-12 00:00:00