Abstract:
:Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the distal end of the respiratory chain. Analysis of the respiratory chain enzyme activities in the presence of GSNO localised the site of inhibition of cytochrome c oxidase alone. These results indicate that nitric oxide is capable of rapidly and reversibly inhibiting the mitochondrial respiratory chain and may be implicated in the cytotoxic effects of nitric oxide in the CNS and other tissues.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cleeter MW,Cooper JM,Darley-Usmar VM,Moncada S,Schapira AHdoi
10.1016/0014-5793(94)00424-2subject
Has Abstractpub_date
1994-05-23 00:00:00pages
50-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00424-2journal_volume
345pub_type
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