Abstract:
:A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated region 1322-base pair 3'-ultranslated region including 20 nucleotides of poly A tail in the total length of 2873 base pairs. The deduced amino acid sequence showed 82% similarity to rat P-450ch7 alpha. Unique amino acid residues were observed in putative binding domains for heme and steroid which are highly conserved in most steroidogenic P-450s.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Noshiro M,Okuda Kdoi
10.1016/0014-5793(90)80992-rsubject
Has Abstractpub_date
1990-07-30 00:00:00pages
137-40issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(90)80992-Rjournal_volume
268pub_type
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