Abstract:
:The relationship between the Fc epsilon receptor mediated stimulation of mast cells and the Ca2+ signal it induces were studied using thapsigargin (TG), a blocker of the endoplasmic reticulum Ca2+ pump. TG induced, in mucosal mast cells (RBL-2H3 line), a dose-dependent and an InsP3-independent increase in [Ca2+]i (from resting levels of 83-150 nM to 600-680 nM), and a secretory response amounting to 30-50% of that observed upon Fc epsilon RI clustering. The TG induced rise of [Ca2+]i is most probably provided by both arrest of its uptake by the endoplasmic reticulum and influx from the medium. Thus, Ca2+ influx in mast cells may be modulated by the [Ca2+]i level.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dar O,Pecht Idoi
10.1016/0014-5793(92)81311-9keywords:
subject
Has Abstractpub_date
1992-09-28 00:00:00pages
123-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(92)81311-9journal_volume
310pub_type
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