Abstract:
:500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-alpha CH spin-spin coupling constants allowed prediction of the secondary structure. Aspects of the tertiary structure are also identified. A pair of antiparallel beta-sheets involving residues 18-23 and 28-34 is a dominant feature of the solution structure.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Carver JA,Cooke RM,Esposito G,Campbell ID,Gregory H,Sheard Bdoi
10.1016/0014-5793(86)80869-9subject
Has Abstractpub_date
1986-09-01 00:00:00pages
77-81issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(86)80869-9journal_volume
205pub_type
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