Calorimetric characterization of the stable complex of myosin subfragment 1 with ADP and beryllium fluoride.

abstract：:Hic-5/ARA55 is a LIM-only member of the paxillin superfamily. Conflicting reports have suggested that Hic-5/ARA55 can both repress and enhance a number of biological processes, including myogenesis and tumorigenesis. With two Hic-5 isoforms documented, we hypothesized that multiple Hic-5 isoforms may exist that have b...

journal_title：FEBS letters

authors： Gao Z,Schwartz LM

更新日期：2005-10-24 00:00:00

abstract：:Mod5 is a multifunctional protein that modifies a subset of tRNAs in the cytoplasm and is also required for an RNA-mediated form of transcriptional silencing. Previous in vivo studies have shown that the nuclear silencing function of Mod5 does not require that the causative tRNA gene encode a Mod5 substrate, although ...

journal_title：FEBS letters

authors： Read DF,Waller TJ,Tse E,Southworth DR,Engelke DR,Smaldino PJ

更新日期：2017-06-01 00:00:00

abstract：:We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...

journal_title：FEBS letters

authors： Kusakabe T,Suzuki N

更新日期：2000-10-20 00:00:00

abstract：:The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...

journal_title：FEBS letters

authors： Wilmen A,Göke B,Göke R

更新日期：1996-11-25 00:00:00

abstract：:Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylo...

journal_title：FEBS letters

authors： Desiderio MA,Dansi P,Tacchini L,Bernelli-Zazzera A

更新日期：1999-07-16 00:00:00

abstract：:Alpha-toxins from scorpion venoms prolong the action potential of excitable cells by blocking sodium channel inactivation. We have purified bukatoxin, an alpha-toxin from scorpion (Buthus martensi Karsch) venom, to homogeneity. Bukatoxin produced marked relaxant responses in the carbachol-precontracted rat anococcygeu...

journal_title：FEBS letters

authors： Srinivasan KN,Nirthanan S,Sasaki T,Sato K,Cheng B,Gwee MC,Kini RM,Gopalakrishnakone P

更新日期：2001-04-13 00:00:00

abstract：:Dynein is a minus end-directed microtubule motor that serves multiple cellular functions. We have performed a fine mapping of the 8 kDa dynein light chain (LC8) binding sites throughout the development of a library of consecutive synthetic dodecapeptides covering the amino acid sequences of the various proteins known ...

journal_title：FEBS letters

authors： Rodríguez-Crespo I,Yélamos B,Roncal F,Albar JP,Ortiz de Montellano PR,Gavilanes F

更新日期：2001-08-17 00:00:00

abstract：:The nitration of tyrosine residues in protein occurs through the action of reactive oxygen and nitrogen species and is considered a marker of oxidative stress under pathological conditions. The most active nitrating species so far identified is peroxynitrite, the product of the reaction between nitric oxide and supero...

journal_title：FEBS letters

authors： Mallozzi C,Di Stasi AM,Minetti M

更新日期：2001-08-17 00:00:00

abstract：:Heat shock proteins (Hsps) are induced in response to various kinds of environmental and physiological stresses. However, it is unclear whether Hsps play roles in protecting cells in the digestive organs against xenobiotic chemicals. Here, we found that feeding induces expression of a set of Hsps specifically in the m...

journal_title：FEBS letters

authors： Katsuki K,Fujimoto M,Zhang XY,Izu H,Takaki E,Tanizawa Y,Inouye S,Nakai A

更新日期：2004-07-30 00:00:00

abstract：:Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...

journal_title：FEBS letters

authors： Roth B,Schwendimann B,Hughes GJ,Tzartos SJ,Barkas T

更新日期：1987-08-31 00:00:00

abstract：:Jun activation domain-binding protein 1 (Jab1) is a coactivator of activating protein-1 (AP-1) and is the fifth component of the COP9 signalosome complex. It interacts with a variety of proteins and plays important roles in diverse signaling pathways and cellular function including oncogenesis. We show here that Jab1 ...

journal_title：FEBS letters

authors： Wang H,Sun X,Luo Y,Lin Z,Wu J

更新日期：2006-10-30 00:00:00

abstract：:Hydrolysis of GTP, bound to members of the G-protein superfamily, terminates their downstream signaling activity. A conserved glutamine serves a critical role in this pivotal guanosine triphosphatase (GTPase) reaction. However, the role of the catalytic glutamine in GTP hydrolysis is still not well understood. We have...

journal_title：FEBS letters

authors： Zor T,Andorn R,Sofer I,Chorev M,Selinger Z

更新日期：1998-08-21 00:00:00

abstract：:The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...

journal_title：FEBS letters

authors： Boatright JH,Knox BE,Jones KM,Stodulkova E,Nguyen HT,Padove SA,Borst DE,Nickerson JM

更新日期：2001-08-24 00:00:00

abstract：:The retromer complex is a key element of the endosomal protein sorting machinery that is conserved through evolution and has been shown to play a role in diseases such as Alzheimer's disease and Parkinson's disease. Through sorting various membrane proteins (cargo), the function of retromer complex has been linked to ...

journal_title：FEBS letters

authors： Mukadam AS,Seaman MN

更新日期：2015-09-14 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands have been shown to inhibit the effects of proinflammatory cytokines such as interleukin-1beta (IL-1beta). This cytokine plays a key role in articular pathophysiologies by inducing the production of inflammatory mediators such as nitric oxide (NO) and...

journal_title：FEBS letters

authors： Boyault S,Bianchi A,Moulin D,Morin S,Francois M,Netter P,Terlain B,Bordji K

更新日期：2004-08-13 00:00:00

abstract：:STAT3, a transcription factor involved in various physiological and pathological processes, is also present in mitochondria. Mitochondrial STAT3 regulates complex I activity and reactive oxygen species (ROS) production, yet the mechanisms governing its translocation to mitochondria remain poorly understood. In this st...

journal_title：FEBS letters

authors： Mohammed F,Gorla M,Bisoyi V,Tammineni P,Sepuri NBV

更新日期：2020-05-01 00:00:00

abstract：:xxR/KxRxxSxx sequences were phosphorylated with high efficiency by both p70 S6 kinase (p70S6K) and MAPKAP kinase-1. The best substrate for MAPKAP kinase-1 (KKKNRTLSVA) was phosphorylated with a Km of 0.17 microM, and the best substrate for p70S6K (KKRNRTLSVA) with a Km of 1.5 microM. The requirement of both enzymes fo...

journal_title：FEBS letters

authors： Leighton IA,Dalby KN,Caudwell FB,Cohen PT,Cohen P

更新日期：1995-11-20 00:00:00

abstract：:We identified a CpG island at the 5' end of murine leukemia inhibitory factor gene (LIF). The CpG island is 0.6 kb long and covers most of the first exon and first intron. The region is non-methylated, its G+C content is 65% and relative frequency of CpG dinucleotide is 0.7. The block of 150 nucleotides, which is 72% ...

journal_title：FEBS letters

authors： Kaspar P,Dvorák M,Bartůnĕk P

更新日期：1993-03-15 00:00:00

abstract：:The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with ...

journal_title：FEBS letters

authors： Ehrengruber MU,Geiser T,Deranleau DA

更新日期：1994-06-13 00:00:00

abstract：:The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...

journal_title：FEBS letters

authors： Ouzounis C,Kyrpides N

更新日期：1996-07-22 00:00:00

abstract：:A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

journal_title：FEBS letters

authors： Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

更新日期：1994-03-07 00:00:00

abstract：:Analogues of S-prenylated cysteine like N-acetyl-S-trans,trans-farnesyl-L-cysteine (AFC) have previously been shown to inhibit the carboxyl methylation of proteins carrying a C-terminal S-prenylated cysteine residue and to block the endotoxin-activated serum-elicited chemotactic response of mouse macrophages. Here, we...

journal_title：FEBS letters

authors： Scheer A,Gierschik P

更新日期：1993-03-15 00:00:00

abstract：:The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...

journal_title：FEBS letters

authors： Galyov EE,Karlishev AV,Chernovskaya TV,Dolgikh DA,Smirnov OYu,Volkovoy KI,Abramov VM,Zav'yalov VP

更新日期：1991-07-29 00:00:00

abstract：:Three regions of the alpha chain of Torpedo californica acetylcholine receptor (AChR), corresponding to residues alpha 262-276, alpha 388, 408 and alpha 427-437 were synthesized, purified and characterized. The first two peptides have been proposed to occupy inter-transmembrane regions while the third represented the ...

journal_title：FEBS letters

authors： Atassi MZ,Manshouri T,Yokoi T

更新日期：1988-02-15 00:00:00

abstract：:The inhibitor of gamma-glutamyl transpeptidase (gamma-GT) acivicin modulates cellular responses including growth, myeloid maturation and apoptosis. Whether these effects result from the inhibition of gamma-GT enzyme activity remains unclear. We compared the cellular effects of acivicin against a more potent and specif...

journal_title：FEBS letters

authors： Antczak C,Karp DR,London RE,Bauvois B

更新日期：2001-11-16 00:00:00

abstract：:The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the t...

journal_title：FEBS letters

authors： Wilson CM,Mitsumoto Y,Maher F,Klip A

更新日期：1995-07-10 00:00:00

abstract：:Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...

journal_title：FEBS letters

authors： Zühlke H,Behlke J

更新日期：1968-12-01 00:00:00

abstract：:We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...

journal_title：FEBS letters

authors： Vercellone A,Riviere M,Fournié JJ,Puzo G

更新日期：1992-05-25 00:00:00

abstract：:We previously described that NO. leads to destruction of ZnS clusters and release of Zn2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO. leads to an increase of cytoplasmic and nuclear free Zn2+. L929 cells, mouse splen...

journal_title：FEBS letters

authors： Berendji D,Kolb-Bachofen V,Meyer KL,Grapenthin O,Weber H,Wahn V,Kröncke KD

更新日期：1997-03-17 00:00:00

abstract：:A collagenase cleaving native type I [14C]collagen but inactive against the synthetic substrate Pz-Pro-Leu-Gly-Pro-D-Arg was extracted from mineralized human dental tissue. The enzyme specifically degrades native collagen into characteristic products (3/4) and (1/4). Its apparent molecular mass of 68 kDa is relatively...

journal_title：FEBS letters

authors： Dumas J,Hurion N,Weill R,Keil B

更新日期：1985-07-22 00:00:00