The isolated N-terminal extracellular domain of the glucagon-like peptide-1 (GLP)-1 receptor has intrinsic binding activity.

abstract：:The gatC, gatA and gatB genes encoding the three subunits of glutamyl-tRNA(Gln) amidotransferase from Acidithiobacillus ferrooxidans, an acidophilic bacterium used in bioleaching of minerals, have been cloned and expressed in Escherichia coli. As in Bacillus subtilis the three gat genes are organized in an operon-like...

journal_title：FEBS letters

authors： Salazar JC,Zúñiga R,Raczniak G,Becker H,Söll D,Orellana O

更新日期：2001-07-06 00:00:00

abstract：:Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extract...

journal_title：FEBS letters

authors： Mirshahi M,Borgese F,Razaghi A,Scheuring U,Garcia-Romeu F,Faure JP,Motais R

更新日期：1989-12-04 00:00:00

abstract：:Human hepatocellular carcinoma (HCC) is one of the most common tumors worldwide, in which the genetic mechanisms of oncogenesis are still unclear. To investigate whether the genomic DNA copy number alterations may contribute to primary HCC, the cDNA microarray-based comparative genomic hybridization (CGH) analysis was...

journal_title：FEBS letters

authors： Huang J,Sheng HH,Shen T,Hu YJ,Xiao HS,Zhang Q,Zhang QH,Han ZG

更新日期：2006-06-26 00:00:00

abstract：:The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by si...

journal_title：FEBS letters

authors： Stetzer E,Ebbinghaus U,Storch A,Poteur L,Schrattenholz A,Kramer G,Methfessel C,Maelicke A

更新日期：1996-11-11 00:00:00

abstract：:Since the discovery of the new members of the UCP (uncoupling protein) family, UCP2 and UCP3, very few studies have dealt with the regulation of their expression. Bearing this in mind, administration of a single intravenous injection of TNF-alpha (100 microg/kg body weight) to rats resulted in a significant increase i...

journal_title：FEBS letters

authors： Busquets S,Sanchís D,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

更新日期：1998-12-04 00:00:00

abstract：:Homologues of the catalase-peroxidase gene katG and the gene for the non-specific DNA binding protein dpsA were identified downstream of oxyR in Burkholderia pseudomallei. Northern experiments revealed that both katG and dpsA are co-transcribed during oxidative stress. Under conditions where the katG promoter is not h...

journal_title：FEBS letters

authors： Loprasert S,Whangsuk W,Sallabhan R,Mongkolsuk S

更新日期：2003-05-08 00:00:00

abstract：:Cultured rat hepatocytes exhibit acid phospholipase A activity. On the basis of product formation from stereospecifically radiolabeled phosphatidylethanolamine substrates, phospholipases A1 and A2 have been identified with optimal activities at pH 4.5. According to subcellular fractionation studies, the acid phospholi...

journal_title：FEBS letters

authors： Kunze H,Löffler BM,Schmidt M

更新日期：1988-08-29 00:00:00

abstract：:1,2-Didecanoylglycerol (diC10) is taken up by human platelets and sequentially converted to 1,2-didecanoylphosphatidic acid (PA10) and 1,2-didecanoylphosphatidylinositol (PI10). Agents that increase cyclic AMP in platelets, such as prostacyclin and forskolin, sequentially convert diC10 to PA10 and PI10. They decrease ...

journal_title：FEBS letters

authors： Lapetina EG

更新日期：1986-01-20 00:00:00

abstract：:A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

journal_title：FEBS letters

authors： Peuker H,Pette D

更新日期：1993-03-08 00:00:00

abstract：:In cardiac cells the effects of n-3 PUFAs on the whole genome are still unknown despite their recognized cardioprotective effects and ability to modulate gene expression. We have evaluated the effects of n-3 PUFAs supplementation on the global gene expression profile in cultured neonatal rat cardiomyocytes, detecting ...

journal_title：FEBS letters

authors： Bordoni A,Astolfi A,Morandi L,Pession A,Danesi F,Di Nunzio M,Franzoni M,Biagi P,Pession A

更新日期：2007-03-06 00:00:00

abstract：:Anaerobic biosynthesis of carotenoids in the purple facultative photosynthetic bacterium Rhodobacter sphaeroides was studied using mass spectrometry. We have demonstrated that (18)O from H2(18)O was incorporated into the 1-methoxy group of spheroidene and spheroidenone, the two major carotenoids produced by this bacte...

journal_title：FEBS letters

authors： Yeliseev AA,Kaplan S

更新日期：1997-02-10 00:00:00

abstract：:Light and heavy lysosomes of mouse forebrain were separated from each other by centrifugation on a Percoll gradient. Light lysosomes were then freed from mitochondria and membranes by sucrose density gradient centrifugation and further purified by floatation-centrifugation on a sucrose gradient. The final preparations...

journal_title：FEBS letters

authors： Fiorilli A,Siniscalco C,Chiarini A,Di Francesco L,Venerando B,Tettamanti G

更新日期：1991-05-06 00:00:00

abstract：:cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46,026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein ...

journal_title：FEBS letters

authors： Merkulova MI,Andreeva SG,Shuvaeva TM,Novoselov SV,Peshenko IV,Bystrova MF,Novoselov VI,Fesenko EE,Lipkin VM

更新日期：1999-04-30 00:00:00

abstract：:Cholesterol is one of the major lipids of plasma membranes. Recently, we have shown that cholesterol depletion by methyl-beta-cyclodextrin (M beta CD) induces the activation of the Wnt/beta-catenin pathway and enhances myogenic differentiation. Here, we show that M beta CD-conditioned media accelerates myogenesis in a...

journal_title：FEBS letters

authors： Portilho DM,Martins ER,Costa ML,Mermelstein CS

更新日期：2007-12-22 00:00:00

abstract：:Treatment of U937 cells with dolichyl phosphate led to an increase in the activity of the ICE family protease CPP32, accompanied with cleavage of pre-CPP32 to generate p17. Peptide inhibitors YVAD-cmk and Z-Asp-CH2-DCB (specific to ICE) and DEVD-CHO (specific to CPP32) blocked the dolichyl phosphate-induced apoptosis....

journal_title：FEBS letters

authors： Yokoyama Y,Okubo T,Ozawa S,Nagai F,Ushiyama K,Kano I,Shioda M,Kubo H,Takemura M,Namiki H,Yasugi E,Oshima M,Seyama Y,Kano K

更新日期：1997-07-21 00:00:00

abstract：:Vitamin D3, cholecalciferol, induces cell-cell communication via gap junctions in murine fibroblasts (C3H/10T 1/2 cells) at concentrations between 0.01 and 1.0 microM, as assayed by the dye transfer method using Lucifer yellow CH. The extent of induction is similar to that obtained with the positive controls, canthaxa...

journal_title：FEBS letters

authors： Stahl W,Nicolai S,Hanusch M,Sies H

更新日期：1994-09-19 00:00:00

abstract：:Replacement of tryptophan 392 located in the active site cavity of pyruvate decarboxylase (PDC; EC 4.1.1.1) from Zymomonas mobilis by methionine or glutamine yields enzymes with smaller catalytic constants of 8.5 s(-1) and 3.6 s(-1) at 4 degrees C, compared to that of the wild-type enzyme (17 s(-1)). The rate constant...

journal_title：FEBS letters

authors： Tittmann K,Mesch K,Pohl M,Hübner G

更新日期：1998-12-28 00:00:00

abstract：:Three proteinase inhibitor genes have been identified in the rapeseed (Brassica napus) genome. They are highly homologous to other genes of the mustard inhibitor (MSI) family of proteinase inhibitors characteristic of Cruciferae. In germinating seeds, only the transcript of one gene, coding for a trypsin inhibitor, is...

journal_title：FEBS letters

authors： De Leo F,Volpicella M,Sciancalepore M,Gallerani R,Ceci LR

更新日期：2006-02-06 00:00:00

abstract：:Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...

journal_title：FEBS letters

authors： Zuleeg T,Vogtherr M,Schübel H,Limmer S

更新日期：2000-04-28 00:00:00

abstract：:This review focuses on the factors that regulate the proliferation of pancreatic islet beta-cells in vitro, and in particular on the intracellular pathways that convey the mitogenic signal into a proliferative response. Substances as diverse as nutrients, polypeptides, cytokines, adrenergic agents, lithium, phorbol es...

journal_title：FEBS letters

authors： Sjöholm A

更新日期：1992-10-19 00:00:00

abstract：:Analysis of glutamic acid decarboxylase (GDC) (EC 4.1.1.15) from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...

journal_title：FEBS letters

authors： van der Meer RA,Groen BW,Duine JA

更新日期：1989-03-27 00:00:00

abstract：:Artificial spider silk proteins may form fibers with exceptional strength and elasticity. Wrapping silk, or aciniform silk, is the toughest of the spider silks, and has a very different protein composition than other spider silks. Here, we present the characterization of an aciniform protein (AcSp1) subunit named W1, ...

journal_title：FEBS letters

authors： Xu L,Tremblay ML,Orrell KE,Leclerc J,Meng Q,Liu XQ,Rainey JK

更新日期：2013-10-01 00:00:00

abstract：:A novel gene encoding a new H3 histone varian (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3' terminal stem-loop nucleotide sequence that is typical of the...

journal_title：FEBS letters

authors： Fucci L,Forte A,Mancini P,Affaitati A,Branno M,Aniello F,Geraci G

更新日期：1997-04-21 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP c...

journal_title：FEBS letters

authors： Jarmuszkiewicz W,Antos N,Swida A,Czarna M,Sluse FE

更新日期：2004-07-02 00:00:00

abstract：:An essential difference between eukaryotic ferritins and bacterioferritins is that the latter contain naturally, in vivo haem as Fe-protoporphyrin IX. This haem is located in a hydrophobic pocket along the 2-fold symmetry axes and is liganded by two Met 52. However, in in vivo studies, a cofactor has been isolated in ...

journal_title：FEBS letters

authors： de Val N,Herschbach H,Potier N,Dorsselaer AV,Crichton RR

更新日期：2006-11-13 00:00:00

abstract：:Nucleoside analogues previously found to be inactive against the human immunodeficiency virus (HIV) may be activated by simple chemical derivatisation. As part of our effort to deliver masked phosphates inside living cells we have discovered that certain phosphate triester derivatives of inactive nucleoside analogues ...

journal_title：FEBS letters

authors： McGuigan C,Kinchington D,Wang MF,Nicholls SR,Nickson C,Galpin S,Jeffries DJ,O'Connor TJ

更新日期：1993-05-17 00:00:00

abstract：:The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the t...

journal_title：FEBS letters

authors： Wilson CM,Mitsumoto Y,Maher F,Klip A

更新日期：1995-07-10 00:00:00

abstract：:The redox potentials (E(composite function')) of P700 in intact and diethyl ether-treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of +/-2-3 mV. Stepwise removal of antenna pigme...

journal_title：FEBS letters

authors： Zhang Y,Nakamura A,Kuroiwa Y,Kato Y,Watanabe T

更新日期：2008-04-02 00:00:00

abstract：:To elucidate the subunit composition of axonemal inner-arm dynein, we examined a 38 kDa protein (p38) co-purified with a Chlamydomonas inner arm subspecies, dynein d. We found it is a novel protein conserved among a variety of organisms with motile cilia and flagella. Immunoprecipitation using specific antibody verifi...

journal_title：FEBS letters

authors： Yamamoto R,Yanagisawa HA,Yagi T,Kamiya R

更新日期：2006-11-27 00:00:00

abstract：:The interaction of the adrenoleukodystrophy protein (ALDP), mutated in the peroxisomal disorder X-linked adrenoleukodystrophy, and the very long-chain acyl-CoA synthetase (VLACS), the enzyme whose function is missing in this disease, remains obscure. As a first step to studying this interaction in wild type versus ALD...

journal_title：FEBS letters

authors： Berger J,Truppe C,Neumann H,Forss-Petter S

更新日期：1998-03-27 00:00:00