Using lipoate enantiomers and thioredoxin to study the mechanism of the 2-oxoacid-dependent dihydrolipoate production by the 2-oxoacid dehydrogenase complexes.

abstract：:A complete cDNA encoding rabbit uteroglobin was constructed and expressed in MDCK and Caco-2 cells. The MDCK cells secrete uteroglobin in approximately equal amounts to the apical and the basolateral side, whereas the Caco-2 cells secrete uteroglobin mainly to the basolateral side. Both MDCK and Caco-2 cells thus secr...

journal_title：FEBS letters

authors： Vogel LK,Suske G,Beato M,Norén O,Sjöström H

更新日期：1993-09-20 00:00:00

abstract：:The effect of platelet-derived growth factor (PDGF) on Rap1 expression was investigated in rat vascular smooth muscle cells (SMC). First, evidence for Rap1 proteins was shown by their: (i) detection in membranes using a specific anti-Rap1 antibody, (ii) typical shift in electrophoretic mobility as a consequence of red...

journal_title：FEBS letters

authors： Quarck R,Bryckaert M,Magnier C,Corvazier E,Bredoux R,de Gunzburg J,Fontenay M,Tobelem G,Enouf J

更新日期：1994-04-04 00:00:00

abstract：:The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P23...

journal_title：FEBS letters

authors： Madern D,Anselme J,Härtlein M

更新日期：1992-03-24 00:00:00

abstract：:Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...

journal_title：FEBS letters

authors： Kahyo T,Mostoslavsky R,Goto M,Setou M

更新日期：2008-07-23 00:00:00

abstract：:Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

journal_title：FEBS letters

authors： Ferry DR,Glossmann H

更新日期：1982-11-08 00:00:00

abstract：:By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...

journal_title：FEBS letters

authors： Westerholm-Parvinen A,Vernos I,Serrano L

更新日期：2000-12-15 00:00:00

abstract：:Mycobacterium avium subsp. hominissuis (MAH) is an environmental bacterium causing opportunistic infections. The objective of this study was to identify flexible genome regions in MAH isolated from different sources. By comparing five complete and draft MAH genomes we identified a genomic island conferring additional ...

journal_title：FEBS letters

authors： Lahiri A,Sanchini A,Semmler T,Schäfer H,Lewin A

更新日期：2014-11-03 00:00:00

abstract：:Formylmethanofuran dehydrogenase, a key enzyme of methanogenesis, was purified 100-fold from methanol grown Methanosarcina barkeri to apparent homogeneity and a specific activity of 34 mumol.min-1.mg protein-1. Molybdenum was found to co-migrate with the enzyme activity. The molybdenum content of purified preparations...

journal_title：FEBS letters

authors： Karrasch M,Börner G,Enssle M,Thauer RK

更新日期：1989-08-14 00:00:00

abstract：:We have examined the activity of protein disulfide isomerase (PDI) and glutaredoxin (Grx) 1, 2 and 3 from Escherichia coli to catalyze the cleavage of glutathionylated ribonuclease A (RNase-SG) by 1 mM GSH to yield reduced RNase. Apparent Km values for RNase-SG were similar, 2-10 microM, for Grx 1, 3 and PDI but Grx I...

journal_title：FEBS letters

authors： Lundström-Ljung J,Vlamis-Gardikas A,Aslund F,Holmgren A

更新日期：1999-01-25 00:00:00

abstract：:Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different form...

journal_title：FEBS letters

authors： Saalbach G,Schwerdel M,Natura G,Buschmann P,Christov V,Dahse I

更新日期：1997-08-18 00:00:00

abstract：:Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this curre...

journal_title：FEBS letters

authors： Janigro D,Maccaferri G,Meldolesi J

更新日期：1989-09-25 00:00:00

abstract：:We have isolated a potent platelet aggregation inducer from the crude venom of Calloselasma rhodostoma (Malayan pit viper), termed rhodoaggretin, with a novel oligomeric structure consisting of a dimer of C-type lectin-like heterodimers. On the basis of its native molecular mass of 66 kDa, and a M(r) of 30 kDa for its...

journal_title：FEBS letters

authors： Wang R,Kong C,Kolatkar P,Chung MC

更新日期：2001-11-23 00:00:00

abstract：:DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant methylation has been recognized as a key factor in the pathogenesis of human diseases such as cancer. The discovery of modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidati...

journal_title：FEBS letters

authors： Dietzsch J,Feineis D,Höbartner C

更新日期：2018-06-01 00:00:00

abstract：:Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5'-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modi...

journal_title：FEBS letters

authors： Lasch J,Niedermann G,Bogdanov AA,Torchilin VP

更新日期：1987-04-06 00:00:00

abstract：:The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI ...

journal_title：FEBS letters

authors： Larocque S,Brisson JR,Thérisod H,Perry MB,Caroff M

更新日期：2003-01-30 00:00:00

abstract：:The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact ...

journal_title：FEBS letters

authors： Yokoyama C,Takai T,Tanabe T

更新日期：1988-04-25 00:00:00

abstract：:The transport of proteins from the cytoplasm into the nucleus is a multistep process. The nuclear localization sequence (NLS) of a transport substrate associates with the heterodimeric NLS-receptor which binds to a subset of proteins of the nuclear pore complex (NPC). Translocation through the NPC is energy-dependent ...

journal_title：FEBS letters

authors： Schlenstedt G

更新日期：1996-06-24 00:00:00

abstract：:Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation, and no induction of NFkappaB binding activity. Interestingly, H2O2 was able to induce NFkappaB subunit p65 translocation in the nucleus. In contrast, cells exposed to TNF alpha showed enhanced CREP binding activi...

journal_title：FEBS letters

authors： Jornot L,Petersen H,Junod AF

更新日期：1997-10-27 00:00:00

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

journal_title：FEBS letters

authors： Thompson MP,Kim D

更新日期：2004-06-18 00:00:00

abstract：:To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmi...

journal_title：FEBS letters

authors： Su S,Gedamu L,Dehnade F,Zafarullah M

更新日期：1996-10-21 00:00:00

abstract：:Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin...

journal_title：FEBS letters

authors： Kumari S,Dash D

更新日期：2011-07-21 00:00:00

abstract：:Pleckstrin homology (PH) domains are protein modules found in proteins involved in many cellular processes. The majority of PH domain-containing proteins require membrane association for their function. It has been shown that most PH domains interact directly with the cell membrane by binding to phosphoinositides with...

journal_title：FEBS letters

authors： Maffucci T,Falasca M

更新日期：2001-10-12 00:00:00

abstract：:The primary structure of rhodopsin from the octopus Paroctopus defleini has been determined by parallel analysis of the protein and corresponding cDNA. The amino acid sequence is most similar to the recently cloned Drosophila opsins. Similarities to bovine and human opsins are also evident. The transmembrane topology ...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Abdulaev NG,Zolotarev AS,Artamonov ID,Bespalov IA,Dergachev AE,Tsuda M

更新日期：1988-05-09 00:00:00

abstract：:N-Biotinyl phosphatidylethanolamine spin labelled at the C-14 position of the sn-2 chain has been incorporated at a level of 1 mol% in bilayers of dimyristoyl phosphatidylcholine, and the effects on the chain mobility of binding avidin to the biotin lipid headgroup have been studied by electron spin resonance spectros...

journal_title：FEBS letters

authors： Swamy MJ,Marsh D

更新日期：1993-06-07 00:00:00

abstract：:An Mr 21 000 polypeptide, designated APPG, has been purified by reverse-phase, high-performance liquid chromatography (RP-HPLC), from acid extracts of porcine anterior pituitary glands. This acidic protein possesses an isoelectric point of 4.9. Amino acid analysis shows that it is not a glycoprotein and estimates it t...

journal_title：FEBS letters

authors： Hsi KL,Seidah NG,De Serres G,Chrétien M

更新日期：1982-10-18 00:00:00

abstract：:In this work, we investigated electron transport around the photosynthetic pigment-protein complex of Photosystem I (PS I) mediated by external high-potential electron carrier 2,3-dichloro-1,4-naphtoquinone (Cl2 NQ) and ascorbate. It has been demonstrated that the oxidized species of Cl2 NQ and ascorbate serve as inte...

journal_title：FEBS letters

authors： Petrova AA,Trubitsin BV,Boskhomdzhieva BK,Semenov AY,Tikhonov AN

更新日期：2018-07-01 00:00:00

abstract：:We have previously published a simple kinetic model to analyse possible pitfalls in kinetic measurements of H+/O ratios in mitochondria [(1984) FEBS Lett. 178, 187-192]. While this model demonstrated how relative electrode response times may affect the results, it did not adequately describe the kinetics of proton bac...

journal_title：FEBS letters

authors： Krab K,Wikström M

更新日期：1986-06-09 00:00:00

abstract：:Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...

journal_title：FEBS letters

authors： Bartling B,Hilgefort C,Friedrich I,Silber RE,Simm A

更新日期：2003-12-18 00:00:00

abstract：:The Golgi apparatus is known to modify and sort newly synthesized secretory proteins. However, fundamental mysteries remain about the structure, operation, and dynamics of this organelle. Important insights have emerged from studying the Golgi in yeasts. For example, yeasts have provided direct evidence for Golgi cist...

journal_title：FEBS letters

authors： Papanikou E,Glick BS

更新日期：2009-12-03 00:00:00