Abstract:
:An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential generation related to electron transfer between P700 and the terminal iron-sulfur acceptor F(B) was followed by additional electrogenic phases in the microsecond and millisecond time scales, which contribute approximately 20% to the overall electrogenicity. These phases are attributed to the vectorial electron transfer from Pc to the protein-embedded chlorophyll dimer P700(+) within the PsaA/PsaB heterodimer. The observed rate constant of the millisecond kinetic phase exhibited a saturation profile at increasing Pc concentration, suggesting the formation of a transient complex between Pc and PS I with the dissociation constant K(d) of about 80 microM. A small but detectable fast electrogenic phase was observed at high Pc concentration. The rate constant of this phase was independent of Pc concentration, indicating that it is related to a first-order process.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mamedov MD,Mamedova AA,Chamorovsky SK,Semenov AYdoi
10.1016/s0014-5793(01)02615-1keywords:
subject
Has Abstractpub_date
2001-07-06 00:00:00pages
172-6issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02615-1journal_volume
500pub_type
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