Kinetic and spectral properties of pea cytosolic ascorbate peroxidase.

abstract：:Leishmania major promastigotes are parasites endowed with a plasma membrane electrogenic H+ pump and anionic channels. These systems have been thought to contribute to pH homeostasis of parasites and environmental adaptation by mediating extrusion of protons which are either generated metabolically or result from exog...

journal_title：FEBS letters

authors： Vieira L,Cabantchik ZI

更新日期：1995-03-13 00:00:00

abstract：:Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...

journal_title：FEBS letters

authors： Kooistra T,van Hinsbergh V,Havekes L,Kempen HJ

更新日期：1984-05-07 00:00:00

abstract：:This paper reports the genomic analysis of the strain CBS7064 of Pichia sorbitophila, a homothallic diploid yeast. We sequenced 4829 random sequence tags of about 1 kb and compared them to the Saccharomyces cerevisiae gene products. Approximately 1300 nuclear genes, 22 tRNAs, the rDNA locus, and six mitochondrial gene...

journal_title：FEBS letters

authors： de Montigny J,Spehner C,Souciet J,Tekaia F,Dujon B,Wincker P,Artiguenave F,Potier S

更新日期：2000-12-22 00:00:00

abstract：:Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...

journal_title：FEBS letters

authors： Rentsch D,Laloi M,Rouhara I,Schmelzer E,Delrot S,Frommer WB

更新日期：1995-08-21 00:00:00

abstract：:The TOE1 gene was discovered as a target of the Egr1 transcription factor that participates in cell growth regulation through the upregulation of p21 and a cell cycle delay at the G2/M phase. We report here that TOE1 is able to bind to the p53 tumor suppressor protein, specifically interacting with the C terminal tetr...

journal_title：FEBS letters

authors： Sperandio S,Tardito S,Surzycki A,Latterich M,de Belle I

更新日期：2009-07-07 00:00:00

abstract：:Red blood cells from patients homozygotes for hemoglobin S (HbS) have been studied using a computer-controlled microspectrophotometer, which allows measurements of spectra and dynamics to be undertaken in a single erythrocyte. Complete photodissociation of HbCO results in polymerization of intracellular deoxyhemoglobi...

journal_title：FEBS letters

authors： Coletta M,Alayash AI,Wilson MT,Benedetti PA,Evangelista V,Brunori M

更新日期：1988-08-15 00:00:00

abstract：:In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phos...

journal_title：FEBS letters

authors： Wu L,Li H,Jia CY,Cheng W,Yu M,Peng M,Zhu Y,Zhao Q,Dong YW,Shao K,Wu A,Wu XZ

更新日期：2012-04-05 00:00:00

abstract：:Intravenous injections of 25.5 nmol rat amylin into fasted anesthetized rats caused a rapid increase in plasma lactate followed by an increase in plasma glucose; there was a transient fall in blood pressure. Subcutaneous injection of 25.5 nmol amylin also caused increases in lactate and glucose but did not change bloo...

journal_title：FEBS letters

authors： Young AA,Wang MW,Cooper GJ

更新日期：1991-10-07 00:00:00

abstract：:The minimal functional enzyme unit of Na,K-ATPase consists of an alpha-beta complex. The alpha-subunit bears all functional domains of the enzyme and so far a regulatory role for the beta-subunit in the catalytic cycle has not been established. On the other hand, increasing experimental evidence suggests that the beta...

journal_title：FEBS letters

authors： Geering K

更新日期：1991-07-22 00:00:00

abstract：:Stimulation of cultured pituitary cells from a gonadotrope lineage (alpha T3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp6]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the ...

journal_title：FEBS letters

authors： Netiv E,Liscovitch M,Naor Z

更新日期：1991-12-16 00:00:00

abstract：:Transfer RNAs (tRNAs) are ancient macromolecules that have evolved under various environmental pressures as adaptors in translation in all forms of life but also towards alternative structures and functions. The present knowledge on both "canonical" and "deviating" signature motifs retrieved from vertical and horizont...

journal_title：FEBS letters

authors： Pütz J,Giegé R,Florentz C

更新日期：2010-01-21 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:Cytotoxic effects were observed following 4 h incubation of human leukaemic cells with the iron chelator 1-hydroxypyridine-2-thione (omadine). Its cytotoxic activity was comparable to that of the cytotoxic drug doxorubicin. At the same concentration two other effective iron chelators, desferrioxamine and 1,2-dimethyl-...

journal_title：FEBS letters

authors： Kontoghiorghes GJ,Piga A,Hoffbrand AV

更新日期：1986-08-18 00:00:00

abstract：:Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. ...

journal_title：FEBS letters

authors： Hoffman L,Rechsteiner M

更新日期：1997-03-10 00:00:00

abstract：:Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...

journal_title：FEBS letters

authors： Mahon MJ,Shimada M

更新日期：2005-01-31 00:00:00

abstract：:We screened active compounds from natural marine products able to increase PPARalpha/gamma transcriptional activity. Sargaquinoic acid (SQA) and sargahydroquinoic acid (SHQA) from Sargassum yezoense were identified as novel PPARalpha/gamma dual agonists. The binding affinity of SQA with PPARgamma was higher than that ...

journal_title：FEBS letters

authors： Kim SN,Choi HY,Lee W,Park GM,Shin WS,Kim YK

更新日期：2008-10-15 00:00:00

abstract：:The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...

journal_title：FEBS letters

authors： Cordeiro MC,Piqueras R,de Oliveira DE,Castresana C

更新日期：1998-09-04 00:00:00

abstract：:Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M asco...

journal_title：FEBS letters

authors： Kittridge KJ,Willson RL

更新日期：1984-05-07 00:00:00

abstract：:Phospholipase C(gamma)2 (PLCgamma2) is a phospholipid-converting enzyme which, upon receptor stimulation, is activated within membrane-bound signalling complexes. In contrast to the highly ubiquitous PLCgamma1, PLCgamma2 is expressed predominantly in B-lymphocytes. Associated with antigen-coupling receptors it is acti...

journal_title：FEBS letters

authors： Kang JS,Kohlhuber F,Hug H,Marmé D,Eick D,Ueffing M

更新日期：1996-12-09 00:00:00

abstract：:The recently proposed mechanistic concept of a receptor-regulated entrance compartment for hexose transport formed by microvilli on 3T3-L1 adipocytes predicted a preferential localization of glucose transporters in these structures. The cytochalasin B-binding technique was used to determine in basal and insulin-stimul...

journal_title：FEBS letters

authors： Lange K,Brandt U

更新日期：1990-02-26 00:00:00

abstract：:Recent studies have shown that p21-activated kinase 1 (Pak1) phosphorylates estrogen receptor-alpha (ER alpha) at Ser 305 and also promotes its transactivation function. Here, we sought to investigate whether substitution of serine 305 in ER with glutamic acid (ER alpha-S305E), which mimics the phosphorylation state, ...

journal_title：FEBS letters

authors： Balasenthil S,Barnes CJ,Rayala SK,Kumar R

更新日期：2004-06-04 00:00:00

abstract：:Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca(2+) release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca(2+) dependence in [(3)H]ryanodine b...

journal_title：FEBS letters

authors： Nakai J,Gao L,Xu L,Xin C,Pasek DA,Meissner G

更新日期：1999-10-08 00:00:00

abstract：:The synthetic cRNA encoding for the major T lymphocyte K+ channel (Kv1.3) was injected into Xenopus fertilized eggs. Somites from embryos of stage 20-22 (about 40 h post-fertilization at 19 degrees C) were dissociated and myotomal muscle cells were cultured in vitro for 2 days. The whole cell configuration of the tigh...

journal_title：FEBS letters

authors： Honoré E,Guillemare E,Lesage F,Barhanin J,Lazdunski M

更新日期：1994-07-18 00:00:00

abstract：:31P-NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glyco...

journal_title：FEBS letters

authors： Egan W,Barile M,Rottem S

更新日期：1986-08-18 00:00:00

abstract：:The effects of mildly acidic conditions on the free energy of unfolding (DeltaG(u)(buff)) of the pore-forming alpha-hemolysin (alphaHL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography du...

journal_title：FEBS letters

authors： Bortoleto RK,Ward RJ

更新日期：1999-10-15 00:00:00

abstract：:Diphtheria toxin fragment A is able to inhibit protein synthesis in the eukaryotic cell by ADP-ribosylating the diphthamide residue of elongation factor-2 (EF-2) [(1980) J. Biol. Chem. 255, 10710-10720]. The reaction requires NAD as ADP-ribose donor. This work reports on the capacity of an NAD analog, the nicotinamide...

journal_title：FEBS letters

authors： Giovane A,Balestrieri C,Quagliuolo L,Servillo L

更新日期：1985-10-28 00:00:00

abstract：:Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[...

journal_title：FEBS letters

authors： Caruso D,Risé P,Galella G,Regazzoni C,Toia A,Galli G,Galli C

更新日期：1994-05-02 00:00:00

abstract：:Three mutant polypeptides of the type I 37-residue winter flounder 'antifreeze' protein have been synthesized. All four threonine residues in the native peptide were been mutated to serine, valine and glycine respectively and two additional salt bridges were incorporated into the sequences in order to improve aqueous ...

journal_title：FEBS letters

authors： Haymet AD,Ward LG,Harding MM,Knight CA

更新日期：1998-07-03 00:00:00

abstract：:A subcellular fraction, highly enriched in uncoated vesicles (UCV) with high H+-ATPase (EC 3.6.1.34) activity, was isolated from the crude microsomal fraction of rat liver homogenates by discontinuous sucrose gradient centrifugation. The UCV fraction, recovered at the interface of sucrose density 1.08 and 1.10 g/ml, w...

journal_title：FEBS letters

authors： Flatmark T,Haavik J,Grønberg M,Jorunn Kleiveland L,Wahlstrøm Jacobsen S,Berge SV

更新日期：1985-09-02 00:00:00

abstract：:Interleukin-22 (IL-22) plays an important role in the regulation of immune and inflammatory responses in mammals. The IL-22 binding protein (IL-22BP), a soluble receptor that specifically binds IL-22, prevents the IL-22/interleukin-22 receptor 1 (IL-22R1)/interleukin-10 receptor 2 (IL-10R2) complex assembly and blocks...

journal_title：FEBS letters

authors： de Moura PR,Watanabe L,Bleicher L,Colau D,Dumoutier L,Lemaire MM,Renauld JC,Polikarpov I

更新日期：2009-04-02 00:00:00