Molecular cloning and expression of subunit 9 of the 26S proteasome.


:Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. Western blot analysis showed that the S9-specific antibodies bound the 26S proteasome and its regulatory complex separated on non-denaturing gels. In SDS-PAGE samples of the two complexes, the S9-specific antibodies bound a single 46 kDa subunit. Thus, a cDNA encoding a novel 26S protease subunit has been isolated, sequenced, and expressed.




FEBS letters


Hoffman L,Rechsteiner M




Has Abstract


1997-03-10 00:00:00














  • A mutation affecting carbon catabolite repression suppresses growth defects in pyruvate carboxylase mutants from Saccharomyces cerevisiae.

    abstract::Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Blázquez MA,Gamo FJ,Gancedo C

    更新日期:1995-12-18 00:00:00

  • Ca2+/calmodulin-dependent protein kinase in Saccharomyces cerevisiae.

    abstract::Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. At 0.2 microM beef calmodulin the phosphorylation was optimal at 3 microM free Ca2+, and at 80 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Londesborough J,Nuutinen M

    更新日期:1987-07-13 00:00:00

  • Dimeric and tetrameric forms of catalytically active transmembrane CD38 in transfected HeLa cells.

    abstract::CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bruzzone S,Guida L,Franco L,Zocchi E,Corte G,De Flora A

    更新日期:1998-08-21 00:00:00

  • Silencing of the MT1-MMP/ G6PT axis suppresses calcium mobilization by sphingosine-1-phosphate in glioblastoma cells.

    abstract::The contributions of membrane type-1 matrix metalloproteinase (MT1-MMP) and of the glucose-6-phosphate transporter (G6PT) in sphingosine-1-phosphate (S1P)-mediated Ca(2+) mobilization were assessed in glioblastoma cells. We show that gene silencing of MT1-MMP or G6PT decreased the extent of S1P-induced Ca(2+) mobiliza...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fortier S,Labelle D,Sina A,Moreau R,Annabi B

    更新日期:2008-03-05 00:00:00

  • Monoclonal antibody to desmin purified from cow Purkinje fibers reveals a cell-type specific determinant.

    abstract::We have raised monoclonal antibodies (Mab) to the Mr 55,000 desmin polypeptide, electrophoretically purified from cytoskeletal preparations of isolated bovine heart Purkinje fibers. One of the Mabs, 39AB6, revealed desmin only in cow Purkinje fibers and did not react with desmins from other muscle cells, including ven...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Virtanen I,Närvänen O,Thornell LE

    更新日期:1990-07-02 00:00:00

  • ATP synthesis catalyzed by the mitochondrial F1-F0 ATP synthase is not a reversal of its ATPase activity.

    abstract::The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Syroeshkin AV,Vasilyeva EA,Vinogradov AD

    更新日期:1995-06-05 00:00:00

  • Increased muscarinic receptor binding in heart membranes by an inhibitor of protein kinase C.

    abstract::The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with poly...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Creazzo TL,Wrenn RW

    更新日期:1988-12-19 00:00:00

  • Identification of the genes responsive to etoposide-induced apoptosis: application of DNA chip technology.

    abstract::DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Y,Rea T,Bian J,Gray S,Sun Y

    更新日期:1999-02-26 00:00:00

  • Production of alkenes and novel secondary products by P450 OleTJE using novel H2 O2 -generating fusion protein systems.

    abstract::Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Matthews S,Tee KL,Rattray NJ,McLean KJ,Leys D,Parker DA,Blankley RT,Munro AW

    更新日期:2017-03-01 00:00:00

  • Structural determination of the active site of a sweet protein. A 1H NMR investigation of pMNEI.

    abstract::pMNEI, a single chain sweet protein related to monellin, has been studied by means of 1H NMR at 500 MHz. A partial sequential assignment performed by means of the MCD method allowed the determination of the secondary structure of a large portion of the beta-sheet of pMNEI that contains a likely 'sweet finger': the loo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tancredi T,Iijima H,Saviano G,Amodeo P,Temussi PA

    更新日期:1992-09-21 00:00:00

  • Cholesterol reverts Triton X-100 preferential solubilization of sphingomyelin over phosphatidylcholine: a 31P-NMR study.

    abstract::The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non-solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X-100. Quant...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahyayauch H,Collado MI,Goñi FM,Lichtenberg D

    更新日期:2009-09-03 00:00:00

  • Alternative splicing generates two isoforms of the alpha 2 subunit of the inhibitory glycine receptor.

    abstract::The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

    更新日期:1991-05-20 00:00:00

  • Efficient CD4 binding and immunosuppressive properties of the 13B8.2 monoclonal antibody are displayed by its CDR-H1-derived peptide CB1.

    abstract::A systematic exploration of the V(H)2/V(kappa)12-13 variable domains of the anti-CD4 monoclonal antibody (mAb) 13B8.2 was performed by the Spot method to screen for paratope-derived peptides (PDPs) demonstrating CD4 binding ability. Nine peptides, named CB1 to CB9, were identified, synthesized in a cyclic and soluble ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bès C,Briant-Longuet L,Cerruti M,De Berardinis P,Devauchelle G,Devaux C,Granier C,Chardès T

    更新日期:2001-11-09 00:00:00

  • Ca2+-modulated ONE-GC odorant signal transduction.

    abstract::In a subset of olfactory epithelium the odorant receptor guanylate cyclase, ONE-GC, is a central transduction component of the cyclic GMP signaling pathway. The odorant binds to the extracellular domain and activates its intracellular catalytic domain to generate the odorant second messenger, cyclic GMP. The present s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Duda T,Sharma RK

    更新日期:2009-04-17 00:00:00

  • Chilling followed by incubation at 37 degrees C causes a reduction in NAD+ levels which can be prevented by the poly(ADP-ribose)transferase inhibitor 3-aminobenzamide.

    abstract::The exposure of cells for 60 min to a serum free medium at ice temperature followed by a return to normal culture conditions (30 min at 37 degrees C) caused a dramatic decrease in NAD+ levels. This decrease in NAD+ was prevented by 3-aminobenzamide. Alkaline elution analysis of DNA from cultures that were sisters to t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cantoni O,Sestili P,Cattabeni F,Stocchi V

    更新日期:1986-08-18 00:00:00

  • The functional role of the beta-subunit in the maturation and intracellular transport of Na,K-ATPase.

    abstract::The minimal functional enzyme unit of Na,K-ATPase consists of an alpha-beta complex. The alpha-subunit bears all functional domains of the enzyme and so far a regulatory role for the beta-subunit in the catalytic cycle has not been established. On the other hand, increasing experimental evidence suggests that the beta...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Geering K

    更新日期:1991-07-22 00:00:00

  • Crystal structure of a plant immunophilin domain involved in regulation of MDR-type ABC transporters.

    abstract::We present the three-dimensional structure of the N-terminal FK506-binding protein (FKBP)-like domain of the immunophilin FKBP42 from Arabidopsis thaliana. The data provide the structural background for the explanation of key functional properties reported previously. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Weiergräber OH,Eckhoff A,Granzin J

    更新日期:2006-01-09 00:00:00

  • The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase.

    abstract::Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel electrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Taketani S,Kohno H,Yoshinaga T,Tokunaga R

    更新日期:1989-03-13 00:00:00

  • A novel 45 kDa secretory protein from rat olfactory epithelium: primary structure and localisation.

    abstract::cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46,026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Merkulova MI,Andreeva SG,Shuvaeva TM,Novoselov SV,Peshenko IV,Bystrova MF,Novoselov VI,Fesenko EE,Lipkin VM

    更新日期:1999-04-30 00:00:00

  • The chlorophyll-binding protein IsiA is inducible by high light and protects the cyanobacterium Synechocystis PCC6803 from photooxidative stress.

    abstract::The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions. Here we show that strong light triggers de-repression of isiAB transcription and leads to IsiA and flavodoxin accumulation under iron replete condit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Havaux M,Guedeney G,Hagemann M,Yeremenko N,Matthijs HC,Jeanjean R

    更新日期:2005-04-25 00:00:00

  • Nitric oxide differentially regulates pro- and anti-angiogenic markers in DLD-1 colon carcinoma cells.

    abstract::Inducible nitric oxide (NO) synthase (iNOS) appears to be a marker of tumor progression in colon carcinogenesis. Here we investigated effects of NO on selected chemokines that differentially regulate angiogenesis, namely pro-angiogenic interleukin (IL)-8 as well as tumor-suppressive interferon-inducible protein-10 (IP...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hellmuth M,Paulukat J,Ninic R,Pfeilschifter J,Mühl H

    更新日期:2004-04-09 00:00:00

  • Primary structure of sheep prostaglandin endoperoxide synthase deduced from cDNA sequence.

    abstract::The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yokoyama C,Takai T,Tanabe T

    更新日期:1988-04-25 00:00:00

  • Isolation of less than Glu-Gly-Leu-Arg-Trp-NH2 (Antho-RWamide II), a novel neuropeptide from sea anemones.

    abstract::Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2 (RFamide), a novel peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Gly-Leu-Arg-Trp-NH2, and was named Antho-RWamide II. Antho-RWamide II is a neuropeptide. Its st...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Graff D,Grimmelikhuijzen CJ

    更新日期:1988-10-24 00:00:00

  • The antibacterial peptide seminal plasmin alters permeability of the inner membrane of E. coli.

    abstract::Seminal plasmin (SPLN) a 47-residue peptide, isolated from bovine seminal plasma, exhibits antibacterial activity against Gram-positive and Gram-negative bacteria. Although SPLN strongly inhibits the transcription of various natural and synthetic templates by E. coli RNA polymerase in vitro, it also associates with mo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sitaram N,Krishnakumari V,Nagaraj R

    更新日期:1992-06-01 00:00:00

  • Pyrroloquinoline-quinone: a reactive oxygen species scavenger in bacteria.

    abstract::Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SK

    更新日期:2004-12-03 00:00:00

  • Myotonic dystrophy protein kinase phosphorylates the myosin phosphatase targeting subunit and inhibits myosin phosphatase activity.

    abstract::Myotonic dystrophy protein kinase (DMPK) and Rho-kinase are related. An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly. MYPT1 was phosphorylated by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murányi A,Zhang R,Liu F,Hirano K,Ito M,Epstein HF,Hartshorne DJ

    更新日期:2001-03-30 00:00:00

  • Hyaluronate depolymerization activity induced by progesterone in cultured fibroblasts derived from human uterine cervix.

    abstract::High-molecular-weight [14C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated. [14C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (M(r) about 40,000), whereas that o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tanaka K,Nakamura T,Ikeya H,Higuchi T,Tanaka A,Morikawa A,Saito Y,Takagaki K,Endo M

    更新日期:1994-06-20 00:00:00

  • Nanoparticle self-assembly by a highly stable recombinant spider wrapping silk protein subunit.

    abstract::Artificial spider silk proteins may form fibers with exceptional strength and elasticity. Wrapping silk, or aciniform silk, is the toughest of the spider silks, and has a very different protein composition than other spider silks. Here, we present the characterization of an aciniform protein (AcSp1) subunit named W1, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Xu L,Tremblay ML,Orrell KE,Leclerc J,Meng Q,Liu XQ,Rainey JK

    更新日期:2013-10-01 00:00:00

  • Extracellular release of the 'differentiation enhancing factor', a HMG1 protein type, is an early step in murine erythroleukemia cell differentiation.

    abstract::Differentiation enhancing factor (DEF) is a 29 kDa protein expressed in murine erythroleukemia (MEL) cells and active in promoting a significant increase in the rate of hexamethylenebisacetamide induced differentiation of these cells. The factor was recently shown to possess an amino acid sequence identical to that re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Melloni E,Sparatore B,Patrone M,Pessino A,Passalacqua M,Pontremoli S

    更新日期:1995-07-24 00:00:00

  • An improved electrofusion technique for production of mouse hybridoma cells.

    abstract::An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vienken J,Zimmermann U

    更新日期:1985-03-25 00:00:00