Molecular cloning and expression of subunit 9 of the 26S proteasome.

Abstract:

:Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. Western blot analysis showed that the S9-specific antibodies bound the 26S proteasome and its regulatory complex separated on non-denaturing gels. In SDS-PAGE samples of the two complexes, the S9-specific antibodies bound a single 46 kDa subunit. Thus, a cDNA encoding a novel 26S protease subunit has been isolated, sequenced, and expressed.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Hoffman L,Rechsteiner M

doi

10.1016/s0014-5793(97)00126-9

subject

Has Abstract

pub_date

1997-03-10 00:00:00

pages

179-84

issue

2-3

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(97)00126-9

journal_volume

404

pub_type

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