Abstract:
:The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydrolysis. The latter tRNA is cleaved with much lower yield in the D-loop, and interestingly, cleavage is also detected in the variable region, that is highly resistant to hydrolysis in eukaryotic tRNAs. The possible location of tight Pb, Eu and Mg binding sites in E. coli tRNAPhe is discussed on the basis of the specific hydrolysis data.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Marciniec T,Ciesiołka J,Wrzesinski J,Krzyzosiak WJdoi
10.1016/0014-5793(89)80148-6subject
Has Abstractpub_date
1989-01-30 00:00:00pages
293-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80148-6journal_volume
243pub_type
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