Abstract:
:The use of oryzacystatins I and II, two cysteine proteinase inhibitors naturally produced in rice grains, represents an attractive way for the control of Coleoptera insect pests. The present study was done to analyze the inhibitory effect of recombinant oryzacystatins produced in Escherichia coli as fusion proteins against digestive proteinases of the major pest Colorado potato beetle (Leptinotarsa decemlineata Say). Both inhibitors had a significant effect on total proteolytic activity, but maximal inhibitions ranged from 20 to 80% for pHs varying from 5.0 to 7.0, respectively. This pH-dependent efficiency of plant cystatins was due to the selective inactivation of potato beetle cathepsin H, as demonstrated by the use of inhibitors with different specificities against cathepsins B and H. These results demonstrate the importance of having an adequate knowledge of insect proteinases specifically recognized by the inhibitors to be used in pest control strategies.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Michaud D,Nguyen-Quoc B,Yelle Sdoi
10.1016/0014-5793(93)80320-tsubject
Has Abstractpub_date
1993-09-27 00:00:00pages
173-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80320-Tjournal_volume
331pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80932-7
更新日期:1992-11-09 00:00:00
abstract::The possible role of sphingomyelin cycle for the regulation of cell proliferation was investigated in human keratinocytes. The time-dependent breakdown of sphingomyelin was observed in the immortalized human keratinocyte cell line HaCaT as well as in primary human keratinocytes thereby providing evidence that the sphi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01421-7
更新日期:1996-01-02 00:00:00
abstract::Immunometabolism focuses on the mechanisms regulating the impact of metabolism on lymphocyte activity and autoimmunity outbreak. The adipose tissue is long known to release adipokines, either pro- or anti-inflammatory factors bridging nutrition and immune function. More recently, adipocytes were discovered to also rel...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12733
更新日期:2017-10-01 00:00:00
abstract::By use of the signal sequence trap method, we isolated a cDNA encoding a novel aspartic protease-like protein from the mouse kidney, and termed it 'kidney-derived aspartic protease-like protein (KAP).' The protein, a 419-amino-acid polypeptide with a 16-amino-acid signal sequence, had 47% identity with mouse cathepsin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01473-1
更新日期:1997-01-20 00:00:00
abstract::KOR-3, also known as ORL-1, is a member of the opioid receptor family, encoding the murine receptor for orphanin FQ/nociceptin. In the current studies we have identified five different splice variants of KOR-3 in mouse brain, three of which have not been previously reported. In addition to variants with a 15 bp deleti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01039-4
更新日期:1998-09-11 00:00:00
abstract::Following exposure to 9-(2-phosphonylmethoxyethyl)adenine (an inhibitor of the cellular DNA polymerases alpha, delta and epsilon), human erythroleukemia K562, human T-lymphoid CEM and murine leukemia L1210 cells markedly accumulated in the S phase of the cell cycle. In contrast to DNA replication, RNA synthesis (trans...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00104-0
更新日期:1999-02-19 00:00:00
abstract::The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00815-9
更新日期:1994-08-29 00:00:00
abstract::The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80190-4
更新日期:1987-09-28 00:00:00
abstract::A macrophage migration inhibitory factor (MIF), originally described as a product of activated lymphocytes, has been defined as a 12 kDa protein, expressed in a wide variety of tissues. Here MIF is identified as a phenylpyruvate tautomerase (EC 5.3.2.1) having p-hydroxyphenylpyruvate and phenylpyruvate as its natural ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01261-1
更新日期:1997-11-03 00:00:00
abstract::The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was no...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00697-7
更新日期:1996-08-19 00:00:00
abstract::Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00335-9
更新日期:1997-05-19 00:00:00
abstract::We have used a diethylpyrocarbonate (DEPC) modification [(1976) Prog. Nucl. Acids Res. 16, 189-262] to probe the accessibility of adenines essential for coat protein binding in the MS2 translational operator [(1983) Biochemistry 22, 2601-2610, 2610-2615, 4723-4730; (1987) Biochemistry 26, 1563-1568]. The essential ade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80576-o
更新日期:1991-05-20 00:00:00
abstract::Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling RAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00355-x
更新日期:1998-04-17 00:00:00
abstract::A number of cDNA clones in summary encoding 700 amino acid residues from the N-end of rat liver elongation factor 2 (EF-2) and including 49 nucleotides of the 5'-untranslated mRNA region have been obtained. EF-2 cDNA clones were isolated from gradually constructed small (1000-5000 clones) specific cDNA libraries using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80447-8
更新日期:1989-05-08 00:00:00
abstract::A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81239-k
更新日期:1990-09-17 00:00:00
abstract::Light stimulation of rod cells in vertebrate eyes may cause Ca2+ release from the intracellular disks. Radiolabelled tracers show that light causes a small hyperpolarization of intact disk stacks and redistribution of the ions Ca2+ and Cl-. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80337-9
更新日期:1985-03-25 00:00:00
abstract::The plant toxin ricin has proven valuable as a membrane marker in studies of endocytosis as well as studies of different intracellular transport steps. The toxin, which consists of two polypeptide chains, binds by one chain (the B-chain) to both glycolipids and glycoproteins with terminal galactose at the cell surface...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(99)00529-3
更新日期:1999-06-04 00:00:00
abstract::The phosphatidylinositol 4,5-bisphosphate (PtdIns-P2)-regulated actin-binding protein gelsolin and most phosphoinositide-specific phospholipases C (PLCs) comprise a basic amino acid motif ((K/R)xxxKxK(K/R); x denotes any amino acid) which was previously suggested to represent a PtdInsP2-binding site commonly present i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00464-k
更新日期:1995-05-29 00:00:00
abstract::alpha2-Macroglobulin was derivatized with a sulfhydryl cross-linker and sandwiched between a mica substrate and a silicon nitride tip, both coated with gold, of an atomic force microscope and force curve measurement was carried out. An extensive downward deflection of the cantilever was observed in the retracting real...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00319-5
更新日期:1996-04-29 00:00:00
abstract::Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.036
更新日期:2011-02-18 00:00:00
abstract::Cellular homeostasis is linked tightly to mitochondrial functions. Some damage to mitochondrial proteins and nucleic acids can lead to the depolarization of the inner mitochondrial membrane, thereby sensitizing impaired mitochondria for selective elimination by autophagy. Mitochondrial dysfunction is one of the key as...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.02.060
更新日期:2010-04-02 00:00:00
abstract::The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03501-9
更新日期:2002-11-06 00:00:00
abstract::Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluoresc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80065-6
更新日期:1988-08-29 00:00:00
abstract::A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80111-5
更新日期:1989-01-30 00:00:00
abstract::Leishmania internalize hemoglobin (Hb) via a specific receptor (HbR) for their survival. To identify the Hb-binding domain of HbR, we cloned and expressed several truncated proteins of HbR and determined their ability to bind Hb. Our findings reveal that 90% of Hb-binding activity is retained in HbR41-80 in comparison...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.14027
更新日期:2020-12-14 00:00:00
abstract::Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00868-a
更新日期:1995-09-04 00:00:00
abstract::We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic rece...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.06.012
更新日期:2011-07-21 00:00:00
abstract::Affinity labelling of E. coli ribosomes with the 2',3'-O-[4-(N-2-chloroethyl)-N-methylamino]benzylidene derivative of AUGU6 was studied within the initiation complex (complex I) obtained by using fMet-tRNAMetf and initiation factors and within the pretranslocational complex (complex II) obtained by treatment of comple...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80714-1
更新日期:1986-07-07 00:00:00
abstract::Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.02.043
更新日期:2014-05-02 00:00:00
abstract::Activator protein 1 (AP1) is a complex of Fos and Jun, and it regulates the transcription of genes possessing the AP1-binding sequence. The purpose of this study was to detect living cells that express AP1 after stimulation with a tumor promoter. The Fos and Jun components of AP1 were induced rapidly and transiently i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00487-5
更新日期:1996-06-10 00:00:00