Abstract:
:alpha2-Macroglobulin was derivatized with a sulfhydryl cross-linker and sandwiched between a mica substrate and a silicon nitride tip, both coated with gold, of an atomic force microscope and force curve measurement was carried out. An extensive downward deflection of the cantilever was observed in the retracting realm of the curve, when and only when the substrate was covered with the derivatized protein. The result was interpreted in terms of the mechanical stretching and unfolding of a single or a few protein molecules.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mitsui K,Hara M,Ikai Adoi
10.1016/0014-5793(96)00319-5subject
Has Abstractpub_date
1996-04-29 00:00:00pages
29-33issue
1-2eissn
0014-5793issn
1873-3468journal_volume
385pub_type
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