Abstract:
:1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, initially induced morphological changes similar to those of typical apoptosis in human leukemia HL-60 cells but FPy-treated cells were characterized by the absolute absence of final apoptotic events such as fragmentation into apoptotic bodies. FPy-induced cell death was considered to be apoptotic on the basis of the induction of DNA fragmentation and the protection against these events by the coaddition of a pan-caspase inhibitor. The increase in the cytoplasmic cytochrome c level supported the possibility that FPy-treated cells should have the ability to complete the entire apoptotic process ending in cell fragmentation and apoptotic body formation. At concentrations too low to induce apoptosis, FPy could suppress the induction of apoptotic body formation in HL-60 cells by typical inducers of apoptosis such as actinomycin D or anisomycin. FPy exhibited a cytochalasin-like effect on spatial arrangement of actin filament independent of its apoptosis-inducing activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hamada M,Nishio K,Doe M,Usuki Y,Tanaka Tdoi
10.1016/s0014-5793(02)02373-6keywords:
subject
Has Abstractpub_date
2002-03-13 00:00:00pages
250-4issue
2-3eissn
0014-5793issn
1873-3468pii
S0014579302023736journal_volume
514pub_type
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