Persistent activity modification of phosphatidate phosphohydrolase and fatty acyl-CoA synthetase on incubation of adipocytes with the tumour promoter 12-O-tetradecanoylphorbol 13-acetate.

abstract：:To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PE...

journal_title：FEBS letters

authors： Thiele JJ,Traber MG,Podda M,Tsang K,Cross CE,Packer L

更新日期：1997-01-20 00:00:00

abstract：:Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...

journal_title：FEBS letters

authors： Cheng TP,Murakami N,Elzinga M

更新日期：1992-10-19 00:00:00

abstract：:The alkaline phosphatase of beef, horse, pig and sheep brain have been purified. In each case, the alkaline phosphatase activity can be separated into two or three components by DEAE-cellulose chromatography and by electrophoresis. Although the properties of these enzyme fractions are very similar, there are sufficien...

journal_title：FEBS letters

authors： Brunel C,Cathala G

更新日期：1970-02-16 00:00:00

abstract：:We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...

journal_title：FEBS letters

authors： Sharma Y,Gopalakrishna A,Balasubramanian D,Fairwell T,Krishna G

更新日期：1993-07-12 00:00:00

abstract：:Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stab...

journal_title：FEBS letters

authors： Tachibanaki S,Imai H,Terakita A,Shichida Y

更新日期：1998-03-20 00:00:00

abstract：:The glycine-alanine (GA) repeat of the Epstein-Barr virus nuclear antigen-1 inhibits in cis ubiquitin-dependent proteolysis in mammalian cells through a yet unknown mechanism. In the present study we demonstrate that the GA repeat targets an evolutionarily conserved step in proteolysis since it can prevent the degrada...

journal_title：FEBS letters

authors： Heessen S,Dantuma NP,Tessarz P,Jellne M,Masucci MG

更新日期：2003-12-04 00:00:00

abstract：:Stromal cell-derived factor 1alpha (SDF-1alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this study, we show that SDF-1alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory a...

journal_title：FEBS letters

authors： Ohtsuki T,Hosono O,Kobayashi H,Munakata Y,Souta A,Shioda T,Morimoto C

更新日期：1998-07-17 00:00:00

abstract：:Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...

journal_title：FEBS letters

authors： Bettuzzi S,Strocchi P,Marinelli M,Astancolle S,Davalli P,Corti A

更新日期：1994-07-18 00:00:00

abstract：:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...

journal_title：FEBS letters

authors： Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl D

更新日期：2012-06-04 00:00:00

abstract：:The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F, which are Drosophila homologues of KAI1/CD82 and Tspan11, respectively. These mutants exhibited egg elo...

journal_title：FEBS letters

authors： Han SY,Lee M,Hong YK,Hwang S,Choi G,Suh YS,Park SH,Lee S,Lee SH,Chung J,Baek SH,Cho KS

更新日期：2012-11-16 00:00:00

abstract：:Diphtheria toxin fragment A is able to inhibit protein synthesis in the eukaryotic cell by ADP-ribosylating the diphthamide residue of elongation factor-2 (EF-2) [(1980) J. Biol. Chem. 255, 10710-10720]. The reaction requires NAD as ADP-ribose donor. This work reports on the capacity of an NAD analog, the nicotinamide...

journal_title：FEBS letters

authors： Giovane A,Balestrieri C,Quagliuolo L,Servillo L

更新日期：1985-10-28 00:00:00

abstract：:We describe a Xenopus P2Y receptor that shares only weak homology with members of the mammalian P2Y family, being most similar to human P2Y(11). When activated by nucleotide analogs, it stimulates both calcium and cAMP mobilization pathways, a feature unique, among mammalian P2Y receptors, to P2Y(11). Activity can be ...

journal_title：FEBS letters

authors： Devader C,Drew CM,Geach TJ,Tabler J,Townsend-Nicholson A,Dale L

更新日期：2007-11-13 00:00:00

abstract：:The isolation of a novel biologically active peptide, designated galanin, is described. The peptide was discovered by the detection of its C-terminal amide structure in porcine intestinal extract using a chemical method. It was found that galanin consists of 29 amino acids and the complete amino acid sequence is: Gly-...

journal_title：FEBS letters

authors： Tatemoto K,Rökaeus A,Jörnvall H,McDonald TJ,Mutt V

更新日期：1983-11-28 00:00:00

abstract：:gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a m...

journal_title：FEBS letters

authors： Touqui L,Jandrot-Perrus M,Vargaftig BB

更新日期：1989-09-25 00:00:00

abstract：:In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...

journal_title：FEBS letters

authors： Ye Y,Saburi W,Odaka R,Kato K,Sakurai N,Komoda K,Nishimoto M,Kitaoka M,Mori H,Yao M

更新日期：2016-03-01 00:00:00

abstract：:In developing ideas of how protein structure modifies haem reactivity, the activity of Class I of the plant peroxidase superfamily (including cytochrome c peroxidase, ascorbate peroxidase and catalase-peroxidases (KatGs)) is an exciting field of research. Despite striking sequence homologies, there are dramatic differ...

journal_title：FEBS letters

authors： Zámocký M,Regelsberger G,Jakopitsch C,Obinger C

更新日期：2001-03-16 00:00:00

abstract：:Nine analogs of fusion peptide of influenza virus hemagglutinin whose membrane perturbation activity has been thoroughly tested [Murata et al. (1992) Biochemistry 31, 1986-1992; Murata et al. (1993) Biophys. J. 64, 724-734] were characterized by molecular modeling techniques with the aim of delineating any specific st...

journal_title：FEBS letters

authors： Efremov RG,Nolde DE,Volynsky PE,Chernyavsky AA,Dubovskii PV,Arseniev AS

更新日期：1999-11-26 00:00:00

abstract：:It is shown that conformational changes of receptor proteins brought about by binding of a ligand induce changes in the lipid environment of the receptor that can be monitored by fluorescent lipid probes. On this basis a new approach to studies of ligand-receptor binding is proposed. Using the interaction of the ricin...

journal_title：FEBS letters

authors： Manevich EM,Tonevitsky AG,Bergelson LD

更新日期：1986-01-06 00:00:00

abstract：:The yeast PMR2/ENA1 gene encodes an ATPase involved in sodium extrusion and induced by NaCl. At low salt concentrations (0.3 M) induction is mediated by the HOG-MAP kinase pathway, a system activated by non-specific osmotic stress. At high salt concentrations (0.8 M) induction is mediated by the protein phosphatase ca...

journal_title：FEBS letters

authors： Márquez JA,Serrano R

更新日期：1996-03-11 00:00:00

abstract：:Purified plasma membrane vesicles isolated from multidrug-resistant human KB-V1 cells accumulate [3H]vinblastine in an energy-dependent manner. The accumulation of [3H]vinblastine in the presence of ATP is a saturable process. ATP can be replaced by other purine nucleotide triphosphates, of which GTP is the most effic...

journal_title：FEBS letters

authors： Lelong IH,Padmanabhan R,Lovelace E,Pastan I,Gottesman MM

更新日期：1992-06-15 00:00:00

abstract：:Exposure of human proliferative cells to subcytotoxic stress triggers stress-induced premature senescence (SIPS) which is characterized by many biomarkers of replicative senescence. Proteomic comparison of replicative senescence and stress-induced premature senescence indicates that, at the level of protein expression...

journal_title：FEBS letters

authors： Dierick JF,Kalume DE,Wenders F,Salmon M,Dieu M,Raes M,Roepstorff P,Toussaint O

更新日期：2002-11-20 00:00:00

abstract：:Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...

journal_title：FEBS letters

authors： Thornton C,Jones A,Nair S,Aabdien A,Mallard C,Hagberg H

更新日期：2018-03-01 00:00:00

abstract：:Protective antigen (PA) is the central receptor binding component of anthrax toxin, which translocates catalytic components of the toxin into the cytosol of mammalian cells. Ever since the crystal structure of PA was solved, there have been speculations regarding the possible role of calcium ions present in domain I o...

journal_title：FEBS letters

authors： Gupta PK,Chandra H,Gaur R,Kurupati RK,Chowdhury S,Tandon V,Singh Y,Maithal K

更新日期：2003-11-20 00:00:00

abstract：:Whether all inflammatory agents activate nuclear transcription factors NF-kappaB and activated protein-1 (AP-1) through the same mechanism is not known. We examined the effect of the phosphatidylinositol-3-kinase (PI-3K) inhibitor wortmannin on the activation of NF-kappaB and AP-1 by different inflammatory agents. Wor...

journal_title：FEBS letters

authors： Manna SK,Aggarwal BB

更新日期：2000-05-04 00:00:00

abstract：:We have found that N-acetylneuraminic acid (NANA) consumes toxic hydrogen peroxide (H(2)O(2)) under physiological conditions. Close investigation of this finding revealed that NANA was oxidized by an equimolar amount of H(2)O(2) to provide its decarboxylated product, 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-oct...

journal_title：FEBS letters

authors： Iijima R,Takahashi H,Namme R,Ikegami S,Yamazaki M

更新日期：2004-03-12 00:00:00

abstract：:Antibodies were made to a thirteen amino acid synthetic peptide corresponding to the C-terminal portion of the glutamate (glu) receptor, GluR-A. The immunoprecipitation of kainic acid (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) binding sites by the anti-peptide antibodies was studied using...

journal_title：FEBS letters

authors： Wenthold RJ,Hunter C,Wada K,Dechesne CJ

更新日期：1990-12-10 00:00:00

abstract：:The cytochrome P-450 content of rat hepatocytes declined rapidly over 72 h in culture, due primarily to denaturation to cytochrome P-420. Six different media were investigated for their ability to conserve cytochrome P-450 during culture, and the most successful was a modified Earle's medium. After 72 h culture in thi...

journal_title：FEBS letters

authors： Grant MH,Melvin MA,Shaw P,Melvin WT,Burke MD

更新日期：1985-10-07 00:00:00

abstract：:Friedreich ataxia is the most common recessive neurodegenerative disease and is caused by reduced expression of mitochondrial frataxin. Frataxin depletion causes impairment in iron-sulfur cluster and heme biosynthesis, disruption of iron homeostasis and hypersensitivity to oxidants. Currently no pharmacological treatm...

journal_title：FEBS letters

authors： Lefevre S,Sliwa D,Auchère F,Brossas C,Ruckenstuhl C,Boggetto N,Lesuisse E,Madeo F,Camadro JM,Santos R

更新日期：2012-01-20 00:00:00

abstract：:A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...

journal_title：FEBS letters

authors： Boldin MP,Mett IL,Wallach D

更新日期：1995-06-19 00:00:00

abstract：:Saccharomyces cerevisiae has only one gene encoding a putative voltage-gated Ca2+ channel pore-forming subunit, CCH1, which is not possible to be cloned by conventional molecular cloning techniques using Escherichia coli. Here, we report the successful cloning of CCH1 in yeast by in vivo homologous recombination witho...

journal_title：FEBS letters

authors： Iida K,Tada T,Iida H

更新日期：2004-10-22 00:00:00