Abstract:
:We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bound dye in the 620 nm (J band) region, which is abolished upon the addition of calcium. Replacement of Asp by Asn in the + x position of the weaker calcium-binding site (site I of CaM) abolishes the dye binding, while the same change in the higher affinity site IV attenuates the binding of the dye and does not abolish it. Replacement of Tyr in site IV with Trp does not distort the geometry, although it increases the dye binding affinity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sharma Y,Gopalakrishna A,Balasubramanian D,Fairwell T,Krishna Gdoi
10.1016/0014-5793(93)81761-nsubject
Has Abstractpub_date
1993-07-12 00:00:00pages
59-64issue
1-3eissn
0014-5793issn
1873-3468pii
0014-5793(93)81761-Njournal_volume
326pub_type
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