Inhibition of ubiquitin/proteasome-dependent proteolysis in Saccharomyces cerevisiae by a Gly-Ala repeat.

abstract：:Thioredoxin-interacting protein (Txnip) knockout (TKO) mice exhibit impaired response to fasting. Herein, we showed that activation of adenine monophosphate-activated protein kinase and cellular AMP levels were diminished in the heart and soleus muscle but not in gastrocnemius muscle of fasting TKO mice. Similarly, gl...

journal_title：FEBS letters

authors： Andres AM,Ratliff EP,Sachithanantham S,Hui ST

更新日期：2011-04-20 00:00:00

abstract：:In eukaryotic cells, peroxiredoxins are both antioxidants and regulators of H(2)O(2)-mediated signaling. We previously found that peroxiredoxin-I (Prx-I) was overexpressed in non-small cell lung cancer (NSCLC) tissue. Since overexpressed protein can induce a humoral immune response, we examined whether serum from NSCL...

journal_title：FEBS letters

authors： Chang JW,Lee SH,Jeong JY,Chae HZ,Kim YC,Park ZY,Yoo YJ

更新日期：2005-05-23 00:00:00

abstract：:Uncoupling protein 2 (UCP2) belongs to the family of mitochondrial carriers. Here, we highlight recent findings regarding UCP2 function in the immune system. UCP2 controls immune cell activation by modulating MAPK pathways and the production of mitochondrial reactive oxygen species. In several models of infection, inf...

journal_title：FEBS letters

authors： Emre Y,Nübel T

更新日期：2010-04-16 00:00:00

abstract：:A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...

journal_title：FEBS letters

authors： Kato Y,Ogawa K,Yamamoto S,Abe S,Kishi J,Hayakawa T

更新日期：1990-07-30 00:00:00

abstract：:During mRNA decoding at the ribosome, deviations from stringent codon identity, or "mistranslation," are generally deleterious and infrequent. Observations of organisms that decode some codons ambiguously, and the discovery of a compensatory increase in mistranslation frequency to combat environmental stress have chan...

journal_title：FEBS letters

authors： Moghal A,Mohler K,Ibba M

更新日期：2014-11-28 00:00:00

abstract：:The effect of platelet-derived growth factor (PDGF) on Rap1 expression was investigated in rat vascular smooth muscle cells (SMC). First, evidence for Rap1 proteins was shown by their: (i) detection in membranes using a specific anti-Rap1 antibody, (ii) typical shift in electrophoretic mobility as a consequence of red...

journal_title：FEBS letters

authors： Quarck R,Bryckaert M,Magnier C,Corvazier E,Bredoux R,de Gunzburg J,Fontenay M,Tobelem G,Enouf J

更新日期：1994-04-04 00:00:00

abstract：:In this report, we show for the first time that ceramide-1-phosphate (C1P) stimulates the phosphatidylinositol 3-kinase (PI3-K)/protein kinase B (PKB) pathway, which is a major mechanism whereby growth factors promote cell survival. Also, C1P induced IkappaB phosphorylation, and enhanced the DNA binding activity of th...

journal_title：FEBS letters

authors： Gómez-Muñoz A,Kong JY,Parhar K,Wang SW,Gangoiti P,González M,Eivemark S,Salh B,Duronio V,Steinbrecher UP

更新日期：2005-07-04 00:00:00

abstract：:The regulatory system of smooth muscle thin filaments is thought to involve a major calcium-calmodulin and actin binding protein: caldesmon. A dissective approach was used to isolate a 35 kDa C-terminal fragment of the molecule and to produce antibodies reacting against both the intact and the 15 kDa N-terminal end of...

journal_title：FEBS letters

authors： Harricane MC,Cavadore C,Audemard E,Mornet D

更新日期：1990-08-20 00:00:00

abstract：:Activated B61.SF.1 and CTLL-2 T lymphocyte clones which are strictly dependent on interleukin-2 (IL-2) for growth were used to study the activation of Na+/K+-ATPase. 50% of [3H]thymidine maximal incorporation was obtained when the extracellular concentration of Na+ or K+ was reduced to 50 or 2 mM, respectively. 'Quies...

journal_title：FEBS letters

authors： Redondo JM,López Rivas A,Fresno M

更新日期：1986-10-06 00:00:00

abstract：:Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...

journal_title：FEBS letters

authors： Nikolaev LG,Glotov BO,Dashkevich VK,Barbashov SF,Severin ES

更新日期：1983-10-31 00:00:00

abstract：:Keratins form an intracellular keratin filament network in keratinocytes. Point mutations in the epidermal keratins could lead to the disruption of keratin filament formation, developing skin diseases such as epidermolytic hereditary palmoplantar keratoderma (EHPPK). We found a G to A transition in keratin 9 (K9) cDNA...

journal_title：FEBS letters

authors： Kobayashi S,Tanaka T,Matsuyoshi N,Imamura S

更新日期：1996-05-20 00:00:00

abstract：:Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of alpha-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain ...

journal_title：FEBS letters

authors： Medrano EE,Burrone OR,Ferrer MM,Cafferata EG,Algranati ID

更新日期：1986-09-29 00:00:00

abstract：:Recombinant human interleukin-5 (hIL-5) has been expressed at high levels and produced in large quantities in baculovirus infected Sf9 insect cells. The glycosylated protein was purified using immuno-affinity chromatography and gel filtration. Purified hIL-5 has been crystallized using standard vapour diffusion techni...

journal_title：FEBS letters

authors： Guisez Y,Oefner C,Winkler FK,Schlaeger EJ,Zulauf M,Van der Heyden J,Plaetinck G,Cornelis S,Tavernier J,Fiers W

更新日期：1993-09-27 00:00:00

abstract：:The highly polymorphic major histocompatibility complex class II (MHCII) polypeptides assemble in the ER with the assistance of invariant chain (Ii) chaperone. Ii binds to the peptide-binding pocket of MHCII heterodimers. We explored the mechanism how MHCII subunits attach to Ii. Expression with single alpha or beta s...

journal_title：FEBS letters

authors： Neumann J,Koch N

更新日期：2005-11-07 00:00:00

abstract：:32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...

journal_title：FEBS letters

authors： Bork P

更新日期：1989-10-23 00:00:00

abstract：:Stimulation of human platelets increases cytoplasmic pH (pHi) via activation of Na+/H+ exchange. We have determined the effect of inhibiting Na+/H+ exchange on (i) thrombin-induced Ca2+ mobilization and (ii) turnover of 32P-labelled phospholipids. Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethyliso...

journal_title：FEBS letters

authors： Siffert W,Siffert G,Scheid P,Riemens T,Gorter G,Akkerman JW

更新日期：1987-02-09 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:The main role of voltage-gated proton channels (Hv1) is to extrude protons from the intracellular milieu when, mediated by different cellular processes, the H(+) concentration increases. Hv1 are exquisitely selective for protons and their structure is homologous to the voltage sensing domain (VSD) of other voltage-gat...

journal_title：FEBS letters

authors： Castillo K,Pupo A,Baez-Nieto D,Contreras GF,Morera FJ,Neely A,Latorre R,Gonzalez C

更新日期：2015-11-14 00:00:00

abstract：:Recent studies have shown that p21-activated kinase 1 (Pak1) phosphorylates estrogen receptor-alpha (ER alpha) at Ser 305 and also promotes its transactivation function. Here, we sought to investigate whether substitution of serine 305 in ER with glutamic acid (ER alpha-S305E), which mimics the phosphorylation state, ...

journal_title：FEBS letters

authors： Balasenthil S,Barnes CJ,Rayala SK,Kumar R

更新日期：2004-06-04 00:00:00

abstract：:The primary structure of opsin of the crayfish Procambarus clarkii has been deduced from the cDNA sequence. The opsin is composed of 376 amino acid residues including all the conservative residues characteristic of other members of the rhodopsin family. Comparison of sequences of all known opsins reveals that the majo...

journal_title：FEBS letters

authors： Hariyama T,Ozaki K,Tokunaga F,Tsukahara Y

更新日期：1993-01-11 00:00:00

abstract：:In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...

journal_title：FEBS letters

authors： Montsarrat N,Racaud-Sultan C,Mauco G,Plantavid M,Payrastre B,Breton-Douillon M,Chap H

更新日期：1997-03-03 00:00:00

abstract：:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

journal_title：FEBS letters

authors： Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

更新日期：1994-08-08 00:00:00

abstract：:Metabolic activities of orexin A (OXA) in mature adipocytes are mediated via PI3K/PKB and PPARγ. However, the effects of OXA on preadipocytes are largely unknown. We report here that OXA stimulates the proliferation and viability of 3T3-L1 preadipocytes and protects them from apoptosis via ERK1/2, but not through PKB....

journal_title：FEBS letters

authors： Skrzypski M,Kaczmarek P,Le TT,Wojciechowicz T,Pruszyńska-Oszmalek E,Szczepankiewicz D,Sassek M,Arafat A,Wiedenmann B,Nowak KW,Strowski MZ

更新日期：2012-11-30 00:00:00

abstract：:Trypsin and chymotrypsin have specificity pockets of essentially the same geometry, yet trypsin is specific for basic while chymotrypsin for bulky hydrophobic residues at the P1 site of the substrate. A model by Steitz, Henderson and Blow suggested the presence of a negative charge at site 189 as the major specificity...

journal_title：FEBS letters

authors： Venekei I,Szilágyi L,Gráf L,Rutter WJ

更新日期：1996-01-29 00:00:00

abstract：:The maintenance of membrane lipid composition within strict limits is critical to maintain optimum cellular function. The biophysical properties of the membrane can be influenced among other factors by the saturation/unsaturation of the phospholipid fatty acyl chain. The rate-limiting enzyme in unsaturated fatty acid ...

journal_title：FEBS letters

authors： Hagen RM,Rodriguez-Cuenca S,Vidal-Puig A

更新日期：2010-06-18 00:00:00

abstract：:The gene for human tetranectin was isolated from a genomic library with a mixture of degenerate oligonucleotide probes. The gene is about 12 kbp and contains two intervening sequences. The gene encodes a protein of 202 amino acid residues, with a signal peptide of 21 amino acid residues, followed by the tetranectin se...

journal_title：FEBS letters

authors： Berglund L,Petersen TE

更新日期：1992-08-31 00:00:00

abstract：:The Ras homology (Rho) guanine nucleotide exchange factor p115-RhoGEF couples the alpha(13) heterotrimeric guanine nucleotide binding protein (G protein) subunit to Rho GTPase. Alpha(13) binds to a regulator of G protein signaling (RGS) domain in p115-RhoGEF, but the mechanism of alpha(13) activation of p115-RhoGEF is...

journal_title：FEBS letters

authors： Bhattacharyya R,Wedegaertner PB

更新日期：2003-04-10 00:00:00

abstract：:A microsomal fraction prepared from the mycelia of the band (bd) strain of Neurospora crassa showed enhanced phosphorylation of two small proteins with molecular masses of around 15 kDa (ps15) by the irradiation of the reaction mixture containing [gamma-32P]ATP at 0 degrees C for 1 s with blue light (450 nm, 6 mumol/m...

journal_title：FEBS letters

authors： Oda K,Hasunuma K

更新日期：1994-05-30 00:00:00

abstract：:The C-Jun N-terminal Kinase (JNK) inhibitor SP600125 is widely used to inhibit the JNK-mediated Bax activation and cell apoptosis. However, this report demonstrates that SP600125 synergistically enhances the dihydroartemisinin (DHA)-induced human lung adenocarcinoma cell apoptosis by accelerating Bax translocation and...

journal_title：FEBS letters

authors： Lu YY,Chen TS,Wang XP,Qu JL,Chen M

更新日期：2010-09-24 00:00:00

abstract：:Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...

journal_title：FEBS letters

authors： Dempsey CE,Cryer GD,Watts A

更新日期：1987-06-22 00:00:00