Calpains are involved in phosphatidylinositol 3',4'-bisphosphate synthesis dependent on the alpha IIb beta 3 integrin engagement in thrombin-stimulated platelets.

abstract：:Human immunodeficiency virus type 1 (HIV-1) Rev is a small RNA-binding protein which is essential for viral replication. To investigate the structure of Rev we have mapped the binding sites of a panel of monoclonal antibodies (mAb) by protein footprinting and identified a mAb protecting amino acids within both the N- ...

journal_title：FEBS letters

authors： Jensen TH,Jensen A,Szilvay AM,Kjems J

更新日期：1997-09-01 00:00:00

abstract：:The KlICL1 gene, encoding isocitrate lyase in Kluyveromyces lactis, is essential for ethanol utilization. Deletion analyses identified two functional promoter elements, CSRE-A and CSRE-B. Transcription is activated on ethanol, but not on glucose, glycerol or lactate. Expression depends on the KlCat8p transcription fac...

journal_title：FEBS letters

authors： Rodicio R,López ML,Cuadrado S,Cid AF,Redruello B,Moreno F,Heinisch JJ,Hegewald AK,Breunig KD

更新日期：2008-03-05 00:00:00

abstract：:Autophagy is an evolutionarily conserved stress response that promotes the lysosomal degradation of intracellular components. The bone marrow stromal cell-derived growth inhibitor (BDGI) functions as a stress sensor which is upregulated by oxidative stress and DNA damage. However, the role of BDGI in autophagic respon...

journal_title：FEBS letters

authors： Zhang J,Wang L,Xu J,Tang Y,Huang B,Chen Z,Zhang T,Shen HM,Wu Y,Xia D

更新日期：2020-04-01 00:00:00

abstract：:Fas/FasL interactions have been proposed as a potentially important mechanism mediating beta-cell death in type 1 diabetes. Recent investigations suggest RNA interference, afforded by small interfering RNAs (siRNA), can provide specific and robust gene silencing in mammalian cells. The current study attempted to inves...

journal_title：FEBS letters

authors： Burkhardt BR,Lyle R,Qian K,Arnold AS,Cheng H,Atkinson MA,Zhang YC

更新日期：2006-01-23 00:00:00

abstract：:The Na-Ca exchanger is essential for the Ca2+ homeostasis in many cell types. This transporter has been difficult to investigate because no specific inhibitor is available. We have synthesized an antisense oligodeoxynucleotide directed against the rat cardiac Na-Ca exchanger mRNA. To estimate the activity of the Na-Ca...

journal_title：FEBS letters

authors： Lipp P,Schwaller B,Niggli E

更新日期：1995-05-08 00:00:00

abstract：:The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with ...

journal_title：FEBS letters

authors： Ehrengruber MU,Geiser T,Deranleau DA

更新日期：1994-06-13 00:00:00

abstract：:Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs2SO4 and the composition of proteins which can b...

journal_title：FEBS letters

authors： Gaedigk R,Oehler S,Köhler K,Setyono B

更新日期：1985-01-07 00:00:00

abstract：:The effort to understand the mechanism of uncoupling by UCP has devolved into two models - the fatty acid protonophore model and the proton buffering model. Evidence for each hypothesis is summarized and evaluated. We also evaluate the obligatory requirement for fatty acids in UCP1-mediated uncoupling and the question...

journal_title：FEBS letters

authors： Garlid KD,Jabůrek M,Jezek P

更新日期：1998-10-30 00:00:00

abstract：:We have reported that histone deacetylase (HDAC) inhibitors activate a member of the INK4 family, the p19INK4d gene, causing G1 phase arrest. We report here that HDAC inhibitor, Trichostatin A, activates another member of the INK4 family, the p18INK4c gene, through its promoter in Jurkat cells. Interestingly, the acti...

journal_title：FEBS letters

authors： Yokota T,Matsuzaki Y,Sakai T

更新日期：2004-09-10 00:00:00

abstract：:The human pathogen Vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (CPS) and a translucent phenotype that produces little or no CPS and is attenuated. Here, we found that a V. vulnificus mutant defective for RfaH antitermin...

journal_title：FEBS letters

authors： Garrett SB,Garrison-Schilling KL,Cooke JT,Pettis GS

更新日期：2016-12-01 00:00:00

abstract：:On the basis of recent results, we propose a model for DNA polymerase translocation along DNA. Human immunodeficiency virus reverse transcriptase is taken as an example. According to the model, movement of the enzyme is the result of transition of the enzyme-bound DNA from the A- to B-form which is accompanied by leng...

journal_title：FEBS letters

authors： Wlassoff WA,Dymshits GM,Lavrik OI

更新日期：1996-07-15 00:00:00

abstract：:All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme respons...

journal_title：FEBS letters

authors： Sahin U,Blobel CP

更新日期：2007-01-09 00:00:00

abstract：:The solution structure of the catalytic domain of MMP-20, a member of the matrix metalloproteinases family not yet structurally characterized, complexed with N-Isobutyl-N-(4-methoxyphenylsulfonyl)glycyl hydroxamic acid (NNGH), is here reported and compared with other MMPs-NNGH adducts. The backbone dynamic has been ch...

journal_title：FEBS letters

authors： Arendt Y,Banci L,Bertini I,Cantini F,Cozzi R,Del Conte R,Gonnelli L

更新日期：2007-10-02 00:00:00

abstract：:The vasopressin precursor is composed of 3 domains in line, namely vasopressin, MSEL-neurophysin and a glycopeptide referred to as copeptin, which are separated during the processing. In guinea pig neurohypophysis, the precursor is partially processed so that a two-domain fragment, MSEL-neurophysin--copeptin, can be f...

journal_title：FEBS letters

authors： Chauvet MT,Chauvet J,Acher R

更新日期：1986-03-03 00:00:00

abstract：:Ovulation is a complex process initiated by the surge of the pituitary luteinizing hormone (LH) that provokes the expression of specific genes. We report herein the isolation and characterization of an ovulation-associated, ovary-specific novel isoform of epoxide hydrolase 2 (Ephx2), Ephx2C. This variant is exclusivel...

journal_title：FEBS letters

authors： Shkolnik K,Ben-Dor S,Galiani D,Hourvitz A,Dekel N

更新日期：2007-10-16 00:00:00

abstract：:We present here a combined GC-MS and LC-MS metabolic profiling approach to unraveling the pathological outcomes of aristolochic acid (AA)-induced nephrotoxicity. Urine samples were analyzed by GC-MS and LC-MS in combination with pattern recognition techniques, e.g. principal component analysis (PCA), orthogonal projec...

journal_title：FEBS letters

authors： Ni Y,Su M,Qiu Y,Chen M,Liu Y,Zhao A,Jia W

更新日期：2007-02-20 00:00:00

abstract：:10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...

journal_title：FEBS letters

authors： Tadaki T,Fukushima M,Ushida C,Himeno H,Muto A

更新日期：1996-12-16 00:00:00

abstract：:Using rheumatoid arthritis (RA) and periodontitis mouse models, we demonstrate that RA and periodontitis share many pathological features, such as deregulated cytokine production, increased immune-cell infiltration, increased expression of Toll-like receptors (TLRs), and enhanced osteoclast activity and bone erosion. ...

journal_title：FEBS letters

authors： Hao L,Zhu G,Lu Y,Wang M,Jules J,Zhou X,Chen W

更新日期：2015-05-22 00:00:00

abstract：:Protein glycosylation, which is a key post-translational event, is catalysed by the glycosyltransferase family of enzymes. There is an increasing body of evidence to suggest that these enzymes may themselves be glycosylated, possibly as an autocatalytic event. Using a novel in vitro system, we have investigated the ro...

journal_title：FEBS letters

authors： Breen KC

更新日期：2002-04-24 00:00:00

abstract：:Because phosphorylation of protein kinase C (PKC) may provide a mechanism for regulation of this enzyme, we have examined the ability of two other kinases to phosphorylate PKC. Our results show that casein kinase 1 (CK-1), but not casein kinase 2 (CK-2), can phosphorylate PKC in the absence of Ca2+ and phospholipids. ...

journal_title：FEBS letters

authors： Vila J,Walker JM,Itarte E,Weber MJ,Sando JJ

更新日期：1989-09-11 00:00:00

abstract：:Transactive response DNA-binding protein of 43 kDa (TDP-43) regulates RNA processing, including alternative splicing of tau exon 10. Pathological TDP-43 is hyperphosphorylated. However, how do the protein phosphatase(s) (PP) regulate TDP-43 phosphorylation is unclear. Here, we found that both PP1 and PP2A were coimmun...

journal_title：FEBS letters

authors： Gu J,Wang W,Miao S,Chen F,Wu F,Hu W,Iqbal K,Gong CX,Liu F

更新日期：2018-02-01 00:00:00

abstract：:The Bacillus thuringiensis Cry toxin causes swelling and necrosis in insect cells, but the route(s) and significance of the water influx involved in its cytotoxicity are unclear. Here, we assessed the role of aquaporins (AQPs), known as water channels, in Cry toxin intoxication. An AQP inhibitor did not interfere with...

journal_title：FEBS letters

authors： Endo H,Azuma M,Adegawa S,Kikuta S,Sato R

更新日期：2017-01-01 00:00:00

abstract：:The aim of this study was to investigate the distribution of a calcium-inhibitable adenylyl cyclase type VI (type VI AC) in the central nervous system using an antiserum directed against the N-terminus of type VI AC. Our results indicate that type VI AC immunoreactivity is generally expressed in many brain regions wit...

journal_title：FEBS letters

authors： Liu FC,Wu GC,Hsieh ST,Lai HL,Wang HF,Wang TW,Chern Y

更新日期：1998-09-25 00:00:00

abstract：:The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is...

journal_title：FEBS letters

authors： Korolkov SN,Garnovskaya MN,Basov AS,Chunaev AS,Dumler IL

更新日期：1990-09-17 00:00:00

abstract：:A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...

journal_title：FEBS letters

authors： Koteliansky VE,Gneushev GN,Belkin AM

更新日期：1985-03-11 00:00:00

abstract：:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

journal_title：FEBS letters

authors： Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

更新日期：1999-03-19 00:00:00

abstract：:E. coli GroEL chaperonin monomers, isolated after urea-induced dissociation of GroEL14, undergo cold denaturation below 5 degrees C. Above 5 degrees C, these monomers undergo MgATP-dependent self-assembly. We have demonstrated a conformational transition at 0 degree C induced by interaction of monomeric GroEL with ade...

journal_title：FEBS letters

authors： Lissin NM,Hemmingsen SM

更新日期：1993-06-07 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...

journal_title：FEBS letters

authors： Lebedeva NA,Rechkunova NI,Lavrik OI

更新日期：2011-02-18 00:00:00

abstract：:The efflux of [3H]purines from cultured sympathetic neurons prelabelled with [3H]adenine is accelerated 2-3-fold within hours of nerve growth factor (NGF) withdrawal and is reduced by readdition of NGF. Addition of 8-(4-chlorphenyl-thio) cAMP, which delays neurite degeneration, reduced the enhanced efflux of purines, ...

journal_title：FEBS letters

authors： Tolkovsky AM,Buckmaster EA

更新日期：1989-09-25 00:00:00