Abstract:
:Fas/FasL interactions have been proposed as a potentially important mechanism mediating beta-cell death in type 1 diabetes. Recent investigations suggest RNA interference, afforded by small interfering RNAs (siRNA), can provide specific and robust gene silencing in mammalian cells. The current study attempted to investigate the effects of silencing Fas expression with siRNA on Fas-mediated apoptosis in mouse insulinoma cells following cytokine incubation. Our results indicate that siRNA is capable of rapid inhibition of cytokine-induced Fas mRNA production and cell surface Fas protein. A complete suppression of the total Fas protein was only observed after prolonged incubation with siRNA, suggesting a slow turn-over of Fas protein. Moreover, siRNA significantly inhibited Fas-mediated beta-cell apoptosis assessed by Caspase-3 and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling assays, the extent of which positively correlated with the level of cell surface Fas. These observations provide additional evidence supporting a role for the Fas-mediated pathway in beta-cell destruction, and suggest that siRNA targeting Fas may be of therapeutic value in preventing type 1 diabetes and improving islet cell viability in transplantation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Burkhardt BR,Lyle R,Qian K,Arnold AS,Cheng H,Atkinson MA,Zhang YCdoi
10.1016/j.febslet.2005.12.068keywords:
subject
Has Abstractpub_date
2006-01-23 00:00:00pages
553-60issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01553-Xjournal_volume
580pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Y RNAs are approximately 100 nucleotide long conserved cytoplasmic non-coding RNAs, which produce smaller RNA fragments during apoptosis. Here we show that these smaller RNA molecules are also produced in non-stressed cells and in a range of human cancerous and non-cancerous cell types. Recent reports have speculated ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.03.026
更新日期:2012-04-24 00:00:00
abstract::The glycogen-binding (G) subunit of protein phosphatase-1G is phosphorylated stoichiometrically by glycogen synthase kinase-3 (GSK3), and with a greater catalytic efficiency than glycogen synthase, but only after prior phosphorylation by cyclic AMP-dependent protein kinase (A-kinase) at site 1. The residues phosphoryl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80433-8
更新日期:1989-05-08 00:00:00
abstract::The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.054
更新日期:2005-04-25 00:00:00
abstract::Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00917-x
更新日期:1998-08-21 00:00:00
abstract::ADP-ribosylation of HeLa nonhistone proteins was investigated by using [3H]adenosine as an in vivo radioactive label. The aim was to determine basic differences in the patterns of modification of interphase and metaphase nonhistones. Fluorography revealed a relatively small number of modified proteins for isolated met...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81174-1
更新日期:1985-03-11 00:00:00
abstract::A comparative study has investigated the in vitro dissociation and self-assembly of chaperonin 60 14-mers isolated from E. coli (GroEL), yeast mitochondria and pea chloroplasts. In all cases Mg2+ inhibits, and low temperature stimulates, the urea-induced dissociation. ATP or ADP in the presence of Mg2+ enhance the dis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00151-x
更新日期:1995-03-13 00:00:00
abstract::A novel regulation type, which may be observed as an unusual kinetic 'cooperativity', is predicted for the alpha-keto acid dehydrogenase complexes. The inter-relationship of this regulation with the well-known regulatory effect of enzyme phosphorylation is discussed. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80560-h
更新日期:1993-03-22 00:00:00
abstract::The 39-kDa fragment of oat phytochrome phyA, obtained by tryptic digestion at the amino acids 65 and 425, was studied by resonance Raman spectroscopy. The parent state P(r) reveals far-reaching similarities with that of the native phytochrome implying that the structures of the tetrapyrrole chromophore and its immedia...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02069-x
更新日期:2000-10-06 00:00:00
abstract::Recently, reports have appeared which show structural variations in B-DNA and indicate deviations from a uniform helical structure. We report for the first time that these indications are also present in the B-form fibre diffraction patterns for the lithium salt of natural DNA. We have used an improved method of contr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80922-9
更新日期:1984-10-15 00:00:00
abstract::We used transcriptomic (RNA-seq) analyses to determine whether patients suffering from all types and subtypes of mucopolysaccharidosis (MPS), a severe inherited metabolic disease, may be more susceptible to coronavirus disease 2019 (COVID-19). The expression levels of genes encoding proteins potentially involved in SA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13908
更新日期:2020-10-01 00:00:00
abstract::The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00190-1
更新日期:1996-03-18 00:00:00
abstract::Diapause is an important overwintering strategy enabling Locusta migratoria to survive under stressed conditions. We identified a novel dual-specificity kinase gene that is differentially expressed between long and short day-treated L. migratoria. To determine its function on photoperiodic diapause induction, we clone...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13544
更新日期:2019-11-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) has been recently shown to induce the high-affinity Fc receptor for IgG (Fc(gammaRI)/CD64) in human polymorphonuclear neutrophils (PMN). To elucidate the molecular mechanisms whereby G-CSF exerts this effect, we examined whether the cytokine induces the binding of transcri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00453-x
更新日期:1996-05-20 00:00:00
abstract::We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81077-2
更新日期:1990-10-29 00:00:00
abstract::VASP (vasodilator-stimulated phosphoprotein), a protein associated with microfilaments at cellular contact sites, has been identified as a ligand for profilin and zyxin, two proteins also involved in microfilament dynamics and organization at these regions. Here, we report that VASP also directly binds to vinculin, an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01295-1
更新日期:1996-12-09 00:00:00
abstract::Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.009
更新日期:2013-06-27 00:00:00
abstract::The thermal unfolding of the myosin subfragment 1 (S1) in its stable complex with ADP and beryllium fluoride (S1.ADP.BeF3-) was studied by differential scanning calorimetry. It has been shown that the structure of the S1 molecule in the S1.ADP.BeF3- complex is similar to that of S1 in its complex with ADP and orthovan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80485-d
更新日期:1993-10-11 00:00:00
abstract::For good function, membrane lipids have to be arranged appropriately and be in the correct physical state. In poikilotherms, exposure to cold stress or heat shock can alter membrane properties such that, unless they are corrected quickly, damage and, possibly, death can result. Low temperature stress is countered by m...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.06.066
更新日期:2006-10-09 00:00:00
abstract::cDNA for a novel proton/organic cation transporter, OCTN1, was cloned from human fetal liver and its transport activity was investigated. OCTN1 encodes a 551-amino acid protein with 11 transmembrane domains and one nucleotide binding site motif. It is strongly expressed in kidney, trachea, bone marrow and fetal liver ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01441-5
更新日期:1997-12-08 00:00:00
abstract::Incubation of rhodanese with hche aperonins GroEL and GroES (1:2 GroEL14:GroES7 molar ratio) under functional and steady state conditions for ATP leads to the formation of a high proportion of rhodanese-bound symmetric complexes (GroEL14(GroES7)2), as revealed by native electrophoresis. Aliquots of such samples were o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00186-5
更新日期:1997-03-24 00:00:00
abstract::Calf chymosin molecules exist in the two alternative structural forms: the first one has S1 and S3 binding pockets occluded by its own Tyr77 residue (the self-inhibited form); the second has these pockets free for a substrate binding (the active form). The preliminary incubation of the enzyme with a pentapeptide corre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01488-8
更新日期:1996-01-22 00:00:00
abstract::The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80854-a
更新日期:1992-04-06 00:00:00
abstract::The oxysterols, 7beta-hydroxycholesterol and 7-ketocholesterol, are involved in the cytotoxicity of oxidized LDL. To elucidate their molecular mechanisms, the human promonocytic leukemia cells U937 and U4 were used. U4 cells overexpressing Bcl-2 were obtained by transfection of U937 cells. 7Beta-hydroxycholesterol and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01473-7
更新日期:1997-12-15 00:00:00
abstract::The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple-quantum coherence. We present here an alternative appr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81124-x
更新日期:1989-09-25 00:00:00
abstract::Spermatid-specific thioredoxin-1 (Sptrx-1) is the first member of the thioredoxin family of proteins with a tissue-specific expression pattern, found exclusively in the tail of elongating spermatids and spermatozoa. We describe here further biochemical characterization of human Sptrx-1 protein structure and enzymatic ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03417-8
更新日期:2002-10-23 00:00:00
abstract::Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.044
更新日期:2010-03-19 00:00:00
abstract::Okadaic acid is an inhibitor of the protein Ser/Thr phosphatases PP1 and PP2A, which blocks the activation of extracellular signal-regulated protein kinase 5 (ERK5), a member of the MAP kinase family activated by growth factors and several types of stressors. The blocking of ERK5 activation by okadaic acid was observe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02950-2
更新日期:2002-07-17 00:00:00
abstract::The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81237-2
更新日期:1989-04-10 00:00:00
abstract::Human Tudor staphylococcal nuclease (Tudor-SN) interacts with the G3BP protein and is recruited into stress granules (SGs), the main type of discrete RNA-containing cytoplasmic foci structure that is formed under stress conditions. Here, we further demonstrate that Tudor-SN binds and co-localizes with AGTR1-3'UTR (3'-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.04.045
更新日期:2014-06-13 00:00:00
abstract::The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00463-3
更新日期:1994-06-13 00:00:00