Abstract:
:Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and 12.3 are characterized here. Far-UV CD spectra reveal, as for other sHSPs, predominantly a beta-sheet structure. By gel permeation and crosslinking, they are the first sHSPs shown to occur as tetramers, rather than forming the usual large multimeric complexes. Exceptionally, too, both appear devoid of in vitro chaperone-like abilities. This supports the notion that tetramers are the building blocks of sHSP complexes, and that higher multimer formation, mediated through the N-terminal domains, is a prerequisite for chaperone-like activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kokke BP,Leroux MR,Candido EP,Boelens WC,de Jong WWdoi
10.1016/s0014-5793(98)00917-xsubject
Has Abstractpub_date
1998-08-21 00:00:00pages
228-32issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00917-Xjournal_volume
433pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A cDNA was cloned from a rabbit spleen cDNA library which encoded a G-protein alpha subunit peptide of 374 amino acids, that at the peptide level exhibited 86% and 79% identity with human Galpha16 and mouse G(alpha)15, respectively. The rabbit G(alpha)subunit cDNA was subcloned into a mammalian expression vector and t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01317-4
更新日期:1999-10-22 00:00:00
abstract::We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.080
更新日期:2007-05-29 00:00:00
abstract::Fibromodulin and lumican are closely related members of the extracellular matrix leucine-rich repeat glycoprotein/proteoglycan family. Similar to decorin, another member of this protein family, they bind to fibrillar collagens and function in the assembly of the collagen network in connective tissues. We have studied ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01314-4
更新日期:2000-03-24 00:00:00
abstract::alpha-Crystallin, the major protein of the ocular lens, is known to have extensive similarities to small heat shock proteins and to act as a molecular chaperone. The exposure of hydrophobic surfaces on alpha-crystallin was studied by fluorescence spectroscopy using the hydrophobic probe bis-ANS. Upon heating the prote...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00775-5
更新日期:1995-08-07 00:00:00
abstract::Carboxypeptidase H (CPH) is one of several processing enzymes required for the conversion of peptide hormone precursors into their smaller active forms. In this study, high levels of CPH activity was found in a liver metastasis of a human ileal carcinoid which expresses beta-preprotachykinin mRNA and the tachykinin ne...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80508-8
更新日期:1988-10-10 00:00:00
abstract::To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01146-6
更新日期:1995-11-06 00:00:00
abstract::Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subu...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12886
更新日期:2017-11-01 00:00:00
abstract::The rise of antibiotic resistance combined with the lack of new products entering the market has led to bacterial infections becoming one of the biggest threats to global health. Therefore, there is an urgent need to identify novel antibiotic targets, such as dihydrodipicolinate synthase (DHDPS), an enzyme involved in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13733
更新日期:2020-05-01 00:00:00
abstract::Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an amino acid sequence in human lamp-1 characteristic of a cleavage site for the Neisseria gonorrhoeae IgA1 protease. Furthermo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00163-4
更新日期:1997-03-17 00:00:00
abstract::To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81080-j
更新日期:1993-07-05 00:00:00
abstract::SufC is an ATPase component of the SUF machinery, which is involved in the biosynthesis of Fe-S clusters. To gain insight into the function of this protein, we have determined the crystal structure of Escherichia coli SufC at 2.5A resolution. Despite the similarity of the overall structure with ABC-ATPases (nucleotide...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.058
更新日期:2006-01-09 00:00:00
abstract::Studies of protein synthesis in the chloroplast compartment have revealed a unique combination of translational autoregulations and trans-regulations due to the delivery of a variety of nuclear factors that act post-transcriptionally. We show how these two characteristics concur to set the major step in the regulation...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(02)03260-x
更新日期:2002-10-02 00:00:00
abstract::Tumour necrosis factor-alpha (TNFalpha) is a multifunctional cytokine that exerts a myriad of biological actions in numerous different tissues including adipocytes through its two distinct cell surface receptors. To address the role of each TNF receptor in the biological actions of TNFalpha in adipocytes, we have deve...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01250-3
更新日期:2000-03-03 00:00:00
abstract::In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01859-7
更新日期:2000-08-04 00:00:00
abstract::The herpesvirus life cycle has two distinct phases: latency and lytic replication. The viral immediate early protein replication and transcription activator (RTA) plays a central role in mediating the balance between these two phases. Here, we demonstrate that a B cell terminal differentiation factor X-box binding pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.056
更新日期:2007-07-24 00:00:00
abstract::The ACTH/MSH melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent alpha-MSH analogue to solubilis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81619-b
更新日期:1993-12-20 00:00:00
abstract::We focused on the functional involvement of transforming growth factor-beta-activated kinase 1 (TAK1) in transcriptional regulation of interleukin-2 (IL-2) in T cells. Costimulation of Jurkat cells with 12-O-tetradecanoylphorbol-13-acetate and A23187 leads to a rapid phosphorylation of TAK1 and TAK1-binding protein 1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.10.059
更新日期:2005-12-05 00:00:00
abstract::The role of necrotic and apoptotic pathways in cytolysis mediated by LAK cells was studied. The contribution of necrotic and apoptotic processes to cytolysis depends both on the LAK cells' phenotype and the type of target cells. CD16+/CD8+/CD3- LAK cells induced necrosis of K562 and L929 target cells. The cell death i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80744-f
更新日期:1993-12-06 00:00:00
abstract::The orphan G protein-coupled receptor APJ has been shown to be a coreceptor for human and simian immunodeficiency virus (HIV and SIV) strains. We have determined that some HIV and SIV strains use APJ as a coreceptor to infect the brain-derived NP-2/CD4 cells. Because apelin is an endogenous ligand for the APJ receptor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01487-3
更新日期:2000-05-04 00:00:00
abstract::xxR/KxRxxSxx sequences were phosphorylated with high efficiency by both p70 S6 kinase (p70S6K) and MAPKAP kinase-1. The best substrate for MAPKAP kinase-1 (KKKNRTLSVA) was phosphorylated with a Km of 0.17 microM, and the best substrate for p70S6K (KKRNRTLSVA) with a Km of 1.5 microM. The requirement of both enzymes fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01170-j
更新日期:1995-11-20 00:00:00
abstract::BIR domain and its containing proteins play critical roles in cell apoptosis and cell division. Here several lines of novelty were revealed based on a comprehensive evolutionary analysis of BIR domains in 11 representative organisms. First, the type II BIR domains in Survivin and Bruce showed more conservation compare...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.09.058
更新日期:2008-11-12 00:00:00
abstract::Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. At 0.2 microM beef calmodulin the phosphorylation was optimal at 3 microM free Ca2+, and at 80 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81226-7
更新日期:1987-07-13 00:00:00
abstract::Dinucleotide polyphosphates (ApnA) have emerged as signaling molecules in rapidly dividing cells. The presence and role of Ap5A in the heart remain unknown. Here, we report that the myocardium contains abundant amounts of diadenosine 5',5"-P1,P5-pentaphosphate (Ap5A), a member of the ApnA family. Ischemia induced 10-f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00114-8
更新日期:1998-02-27 00:00:00
abstract::A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80910-u
更新日期:1991-09-02 00:00:00
abstract::Yeast cells lacking the metacaspase-encoding gene YCA1 (Deltayca1) were compared with wild-type (WT) cells with respect to the occurrence, nature and time course of acetic-acid triggered death. We show that Deltayca1 cells undergo programmed cell death (PCD) with a rate lower than that of the WT and that PCD in WT cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.050
更新日期:2006-12-22 00:00:00
abstract::γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12318
更新日期:2016-09-01 00:00:00
abstract::In blood-borne metastasis, intravasated metastatic tumor cells are thought to localize at the target site via a series of processes involving platelet aggregation, adhesion to endothelium, and invasion through the basal membrane. In the present study, we examined how platelet aggregation contributes to the trafficking...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00441-4
更新日期:1998-05-08 00:00:00
abstract::[3H]inositol-labelled products are released from adrenal medullary cells during exocytotic secretion. In 'leaky' cells in which small molecules readily enter and leave the cytoplasm, addition of micromolar calcium ions in the presence of ATP stimulates exocytosis and causes the release of inositol polyphosphates. Thes...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80858-9
更新日期:1985-09-09 00:00:00
abstract::Initiation of translation in hepatitis C virus (HCV) is dependent on the presence of an internal ribosome entry site (IRES) contained in its 341-nt-long 5'-untranslated region (UTR). This region is very conserved among different isolates and has been used to classify HCV isolates in six different genotypes. These geno...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00715-1
更新日期:1997-07-14 00:00:00
abstract::Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.10.021
更新日期:2012-11-30 00:00:00